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氯硝柳胺联合顺铂抑制肾上腺皮质癌的实验研究 被引量:1
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作者 王敏捷 王尉 +2 位作者 朱沂 赵旭 胡卫列 《中国现代医学杂志》 CAS 2018年第26期22-29,共8页
目的研究氯硝柳胺和低剂量顺铂联合应用对人肾上腺皮质癌SW-13细胞株增殖、凋亡,以及迁移力、侵袭力的影响,并探讨其作用机制。方法体外培养SW-13细胞,CCK-8法检测氯硝柳胺和不同浓度顺铂对细胞增殖的影响。设对照组、氯硝柳胺组、顺铂... 目的研究氯硝柳胺和低剂量顺铂联合应用对人肾上腺皮质癌SW-13细胞株增殖、凋亡,以及迁移力、侵袭力的影响,并探讨其作用机制。方法体外培养SW-13细胞,CCK-8法检测氯硝柳胺和不同浓度顺铂对细胞增殖的影响。设对照组、氯硝柳胺组、顺铂组、联合用药组,分别用Annexin V/PI和吖啶橙/溴化乙锭(AO/EB)染色检测细胞凋亡率的变化,Transwell实验和细胞划痕实验检测细胞侵袭、迁移能力的变化,Western blot检测B细胞淋巴瘤因子2(Bcl-2)、半胱氨酸天冬氨酸蛋白酶3(Caspase-3)、波形蛋白(Vimnetin)、E-钙黏蛋白(E-cadherin)表达的变化。结果氯硝柳胺增强顺铂对SW-13细胞增殖的抑制作用。Annexin V/PI和AO/EB染色结果表明,与联合用药组比较,氯硝柳胺、顺铂促进细胞凋亡(P<0.05)。Transwell和划痕实验结果表明,与联合用药组比较,氯硝柳胺、顺铂降低SW-13细胞的侵袭能力(P<0.05)。Western blot检测结果表明,与联合用药组比较,氯硝柳胺、顺铂抑制Bcl-2、Vimentin的表达,促进Caspase-3、E-cadherin的表达(P<0.05)。结论氯硝柳胺能够增强低剂量顺铂对SW-13细胞增殖的抑制作用,促进细胞凋亡,抑制侵袭及迁移能力,可能与其抑制Bcl-2、Caspase-3、E-cadherin、Vimentin的表达有关。 展开更多
关键词 氯硝柳胺 顺铂 SW-13 细胞增殖 细胞凋亡
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1990年与2019年中国病毒性脑炎疾病负担分析 被引量:1
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作者 黄姗姗 周伍明 +3 位作者 汪敏捷 储玉倩 蒋梦可 杨旻 《医学新知》 CAS 2022年第4期241-249,共9页
目的分析中国1990年与2019年病毒性脑炎(viral encephalitis,VE)疾病负担的变化状况,并与同期全球数据进行对比,为我国制定有效的VE防治策略提供参考。方法采用2019年全球疾病负担研究(The Global Burden of Diseases 2019,GBD 2019)数... 目的分析中国1990年与2019年病毒性脑炎(viral encephalitis,VE)疾病负担的变化状况,并与同期全球数据进行对比,为我国制定有效的VE防治策略提供参考。方法采用2019年全球疾病负担研究(The Global Burden of Diseases 2019,GBD 2019)数据,分析VE的发病率、患病率、死亡率、伤残损失健康寿命年(years lived with disability,YLD)率、死亡损失健康寿命年(years of life lost,YLL)率及其标化率,并对比全球变化情况。结果2019年,中国VE发病率为15.27/10万、患病率为74.34/10万、死亡率为0.24/10万、YLD率为7.29/10万、YLL率为13.40/10万,与1990年相比,分别下降27.18%、33.42%、70.00%、40.29%及78.56%。2019年,中国除标化死亡率、YLL率外,其余标化指标均超全球水平。2019年,中国男性标化发病率、患病率、死亡率、YLD率及YLL率分别为22.81/10万、73.74/10万、0.34/10万、7.46/10万、22.26/10万,均高于同期女性。与1990年相似,2019年中国0~4岁人群VE的发病率、死亡率均较其他年龄组高,50岁以上人群发病率、死亡率呈逐渐升高趋势。结论截至2019年,我国VE的疾病负担整体水平较高,在积极降低感染风险的同时,也应重视疾病初期的实验室检查,早发现早治疗以控制病情发展,重点关注儿童和老年男性群体。 展开更多
关键词 病毒性脑炎 疾病负担 发病率 患病率 死亡率
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Pharmacological treatment of inhalation injury after nuclear or radiological incidents: The Chinese and German approach 被引量:1
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作者 Tian-Tian Yan Guo-An Lin +3 位作者 min-jie wang Andreas Lamkowski Matthias Port Alexis Rump 《Military Medical Research》 SCIE CAS CSCD 2019年第2期159-169,共11页
Inhalation injury is often associated with burns and significantly increases morbidity and mortality. The main toxic components of fire smoke are carbon monoxide, hydrogen cyanide, and irritants. In the case of an inc... Inhalation injury is often associated with burns and significantly increases morbidity and mortality. The main toxic components of fire smoke are carbon monoxide, hydrogen cyanide, and irritants. In the case of an incident at a nuclear power plant or recycling facility associated with fire, smoke may also contain radioactive material. Medical treatments may vary in different countries, and in this paper, we discuss the similarities and differences in the treatments between China and Germany. Carbon monoxide poisoning is treated by 100% oxygen administration and,if available, hyperbaric oxygenation in China as well as in Germany. In addition, antidotes binding the cyanide ions and relieving the respiratory chain are important. Methemoglobin-forming agents(e.g., nitrites, dimethylaminophenol)or hydroxocobalamin(Vitamin B12) are options. The metabolic elimination of cyanide may be enhanced by sodium thiosulfate. In China, sodium nitrite with sodium thiosulfate is the most common combination. The use of dimethylaminophenol instead of sodium nitrite is typical for Germany, and hydroxocobalamin is considered the antidote of choice if available in cases of cyanide intoxications by fire smoke inhalation as it does not further reduce oxygen transport capacity. Systematic prophylactic use of corticosteroids to prevent toxic pulmonary edema is not recommended in China or Germany. Stable iodine is indicated in the case of radioiodine exposure and must be administered within several hours to be effective. The decorporation of metal radionuclides is possible with Ca(DTPA)or Prussian blue that should be given as soon as possible. These medications are used in both countries, but it seems that Ca(DTPA) is administered at lower dosages in China. Although the details of the treatment of inhalation injury and radionuclide(s) decorporation may vary, the general therapeutic strategy is very similar in China and Germany. 展开更多
关键词 Fire smoke INHALATION injury Carbon MONOXIDE CYANIDE RADIONUCLIDE incorporation DECORPORATION
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Passively stabilized single-photon interferometer
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作者 刘海龙 王敏杰 +4 位作者 暴佳鑫 刘超 李雅 李淑静 王海 《Chinese Physics B》 SCIE EI CAS CSCD 2022年第11期231-235,共5页
A single-photon interferometer is a fundamental element in quantum information science.In most previously reported works,single-photon interferometers use an active feedback locking system to stabilize the relative ph... A single-photon interferometer is a fundamental element in quantum information science.In most previously reported works,single-photon interferometers use an active feedback locking system to stabilize the relative phase between two arms of the interferometer.Here,we use a pair of beam displacers to construct a passively stable single-photon interferometer.The relative phase stabilization between the two arms is achieved by stabilizing the temperature of the beam displacers.A purely polarized single-photon-level pulse is directed into the interferometer input port.By analyzing and measuring the polarization states of the single-photon pulse at the output port,the achieved polarization fidelity of the interferometer is about 99.1±0.1%.Our passively stabilized single-photon interferometer provides a key element for generating highfidelity entanglement between a photon and atomic memory. 展开更多
关键词 passively stable single-photon interferometer atom-photon entanglement polarization fidelity
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Image Steganalysis System optimization Based on Boundary Samples
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作者 Li-Na wang min-jie wang +1 位作者 Ting-Ting Zhu Qing Dou 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2014年第6期57-62,共6页
In the image steganalysis,the training samples often determine the performance of the model when the features and classification are in the same condition.However the existing research on steganalysis lacks the in-dep... In the image steganalysis,the training samples often determine the performance of the model when the features and classification are in the same condition.However the existing research on steganalysis lacks the in-depth study of the classifier's training method which may deeply influence the detection performance.This paper provides an optimization of universal steganalysis based on the boundary samples classification concerning about image steganalysis.This paper proposes a strategy of selecting boundary samples in steganalysis and divides the training samples into good samples,poor samples and boundary samples three categories and then chose the optimal threshold to get boundary samples through experiments.The experimental results show the effectiveness of boundary sample,which dramatically improve detection capability especially for the low embedding rate Stego-image. 展开更多
关键词 image steganalysis digital forensics support vector machine(SVM) boundary samples
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Adiabatic shear fracture prediction in high-speed cuttingat various negative rake angles and feeds
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作者 Li-Yao Gu min-jie wang 《Advances in Manufacturing》 SCIE CAS CSCD 2018年第1期41-51,共11页
The critical characteristics of adiabatic shearfracture (ASF) that induce the formation of isolated seg-ment chip in high-speed machining was further investi-gated. Based on the saturation limit theory, combining wi... The critical characteristics of adiabatic shearfracture (ASF) that induce the formation of isolated seg-ment chip in high-speed machining was further investi-gated. Based on the saturation limit theory, combining withthe stress and deformation conditions and the modifiedJohnson-Cook constitutive relation, the theoretical predic-tion model of ASF was established. The predicted criticalcutting speeds of ASF in high-speed machining ofa hardened carbon steel and a stainless steel were verifiedthrough the chip morphology observations at various neg-ative rake angles and feeds. The influences of the cuttingparameters and thermal-mechanical variables on theoccurrence of ASF were discussed. It was concluded thatthe critical cutting speed of ASF in the hardened carbonsteel was higher than that in the stainless steel under alarger feed and a lower negative rake angle. The proposedprediction model of ASF could predict reasonable results ina wide cutting speed range, facilitating the engineeringapplications in high-speed cutting operations. 展开更多
关键词 Stress wave Saturation limit FRACTURE Isolated segment Prediction
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