Analysis of inflammation, antioxidant, insulin resistance and bone metabolism in patients with chronic periodontitis

Objective:To investigate the changes and clinical significance of inflammation, antioxidation, insulin resistance and bone metabolism in patients with chronic periodontitis.Methods: 50 patients with chronic periodontitis treated in our hospital from January 2017 to March 2018 were selected as the observation group and 50 cases of periodontal health were selected as control group. The expression levels of related indicators of inflammation [including macrophage migration inhibitory factor (MIF), monocyte chemoattractant protein-1 (MCP-1), granulocyte-macrophage colony-stimulating factor (GM-CSF)], antioxidant [including nitric oxide (NO) and nitric oxide synthase (NOS)], insulin resistance [including adiponectin, leptin (LEP)] and bone metabolism [including visfatin and calcitonin gene-related peptide (CGRP)] in the two groups were observed and compared.Results: Except for the levels of adiponectin [(3.03±0.40) ng/L] and CGRP [(32.40±12.61) μg/L] in the observation group [(5.33±0.63) ng/L and (49.84±13.36) μg/L, respectively] were significantly lower than those in the control group (P<0.05). The levels of MIF [(14.15±4.40) ng/mL], MCP-1 [(0.93±0.13) μg/L], GM-CSF [(1.71±0.51) μg/L], NO [(84.67±26.02) μmol/L], NOS [(49.71±9.52) μ/mL], LEP [(0.88±0.27) μg/L] and visfatin [(80.34±33.57) μg/L] were significantly higher than those in the control group ((11.27±1.95) ng/mL, (0.51±0.07) μg/L, (0.45±0.17) μg/L, (54.80±9.33) μmol/L, (30.29±4.63) μ/mL, (0.47±0.11) μg/L and (34.61±14.02) μg/L, respectively)All above differences were significant (P<0.05).Conclusions: Patients with chronic periodontitis are prone to inflammatory reaction. And the degree of inflammation is deeper. It is easy to stimulate antioxidant effect and resist periodontal infection. It may also aggravate insulin resistance to increase blood sugar or affect bone metabolism and osteoporosis.

Detection and analysis of T cell subsets, T cell cytokine and immunoglobulin in patients with oral cancer before and after operation

Objective:To investigate the changes of T cell subsets, T cell cytokine and immunoglobulin in patients with oral cancer before and after operation.Methods:A total of 40 cases of oral cancer treated in our hospital from January 2017 to December 2017 were selected as the observation group, and 40 healthy volunteers in our hospital were selected as the control group. The levels of T cell subsets, T cell cytokine and immunoglobulin in the observation group before and after operation were detected and compared with those in the control group.Results:The CD3+, CD4+ and CD4+/ CD8+ of the observation group before operation were lower than those of the control group, and CD8+ was higher than that of the control group, the differences were statistically significant. The CD3+, CD4+ and CD4+/CD8+ after operation in the observation group were (64.98 ± 3.88)%, (42.40 ± 3.53)% and (1.56 ± 0.41) respectively, which were all higher than those before the operation, but still lower than those in the control group. The CD8+ of the observation group was (27.25 ± 3.54)% lower than that before operation, but it was still higher than that of the control group. In the observation group, the levels of INF-γ+, CD4+/INF-γ+, IL-2+, and CD4+/IL-2+ before operation were lower in the observation group than those in the control group. In the observation group, INF-γ+, CD4+/INF-γ+, IL-2+, and CD4+/IL-2+ after surgery were (0.15±0.06)%, (0.07±0.03)%, (0.47±0.13)%, (0.16 ± 0.11)%, were higher than those before surgery, but still lower than those in the control group. The IgA and IgG before and after the surgery in the observation group were (2.01±0.13) g/L, (1.81±0.12) g/L, (10.53±3.12) g/L, and (8.41±2.01) g/L, respectively, compared with the control group, the difference was statistically significant. There was no significant change in IgA, IgG and IgM in the observation group before and after operation.Conclusion:Surgery can effectively improve the T lymphocyte subsets and T cell cytokine levels in oral cancer patients, and has a positive effect on improving immune function.