OBJECTIVE: To investigate the gene expression of 4-1BB in peripheral blood mononuclear cells(PBMCs) and its possible significance in clinical liver transplantation.METHODS: Reverse transcription-polymerase chain react...OBJECTIVE: To investigate the gene expression of 4-1BB in peripheral blood mononuclear cells(PBMCs) and its possible significance in clinical liver transplantation.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the geneexpression of 4-1BB in PBMCs from 22 patients receiving liver transplantation, 13 patients with primaryliver carcinoma (PLC), and 12 healthy controls. To determine whether 4-1BB molecule is also expressedon the surface of CD4^+ and CD8^+ T cell, flow cytometry was used to analyse the phenotype of T cellsubsets from the blood of liver transplantation patients.RESULTS: 4-1BB mRNA was detected in PBMCs from stable survivors after liver transplantation, butalmost not deteeted in PBMCs from PLC patients and healthy controls. Meanwhile, 4-1BB was almost notexpressed on the surface of CD4^+ and CD8^+ T cells in healthy controls and PLC patients. A low level of4-1BB expression, however, was found on the surface of CD4^+ and CD8^+ T cells from the stablesurvivors after liver transplantation.CONCLUSIONS: This study demonstrates that although patients are stable after liver transplantation,effector T-cells can also be activated through the signal of 4-1BB molecule and persistent irmmune responseto grafts. Blockage of 4-1BB/4-1BBL pathway may benefitially reduce the clinical dosage ofimmunosuppressive agents and prolong the survival of grafts.展开更多
Herein,solid lipid nanoparticles(SLN)were proposed as a new drug delivery system for adefovir dipivoxil(ADV). The octadecylamine-fluorescein isothiocynate(ODA-FITC)was synthesized and used as a fluorescence maker to b...Herein,solid lipid nanoparticles(SLN)were proposed as a new drug delivery system for adefovir dipivoxil(ADV). The octadecylamine-fluorescein isothiocynate(ODA-FITC)was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15.The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system.About 15 wt%drug entrapment efficiency(EE)and 3 wt% drug loading(DL)could be reached in SLN loading ADV.Comparing with free ADV,the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen(HBsAg),hepatitis B e antigen(HBeAg)and hepatitis B virus(HBV)DNA levels in vitro were significantly enhanced.展开更多
文摘OBJECTIVE: To investigate the gene expression of 4-1BB in peripheral blood mononuclear cells(PBMCs) and its possible significance in clinical liver transplantation.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the geneexpression of 4-1BB in PBMCs from 22 patients receiving liver transplantation, 13 patients with primaryliver carcinoma (PLC), and 12 healthy controls. To determine whether 4-1BB molecule is also expressedon the surface of CD4^+ and CD8^+ T cell, flow cytometry was used to analyse the phenotype of T cellsubsets from the blood of liver transplantation patients.RESULTS: 4-1BB mRNA was detected in PBMCs from stable survivors after liver transplantation, butalmost not deteeted in PBMCs from PLC patients and healthy controls. Meanwhile, 4-1BB was almost notexpressed on the surface of CD4^+ and CD8^+ T cells in healthy controls and PLC patients. A low level of4-1BB expression, however, was found on the surface of CD4^+ and CD8^+ T cells from the stablesurvivors after liver transplantation.CONCLUSIONS: This study demonstrates that although patients are stable after liver transplantation,effector T-cells can also be activated through the signal of 4-1BB molecule and persistent irmmune responseto grafts. Blockage of 4-1BB/4-1BBL pathway may benefitially reduce the clinical dosage ofimmunosuppressive agents and prolong the survival of grafts.
文摘Herein,solid lipid nanoparticles(SLN)were proposed as a new drug delivery system for adefovir dipivoxil(ADV). The octadecylamine-fluorescein isothiocynate(ODA-FITC)was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15.The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system.About 15 wt%drug entrapment efficiency(EE)and 3 wt% drug loading(DL)could be reached in SLN loading ADV.Comparing with free ADV,the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen(HBsAg),hepatitis B e antigen(HBeAg)and hepatitis B virus(HBV)DNA levels in vitro were significantly enhanced.
