Exploration on the establishment of animal models for hyperuricemia and the application of traditional Chinese medicine

OBJECTIVE In recent years,the frequency of hyperuricemia has gradually risen along with the improvement of living standards,the irregular of diet and overfeeding greasy and surfeit flavor.However,hyperuricemia is a disorder of purine metabolism,and is strongly associated with insulin resistance and abnormal glucose metabolism.It is important to obtain a more stable and sustained animal model for the efficacy evaluation of traditional Chinese medicine(TCM).METHODS To manufacture the rodent model of hyperuricemia,the theory of increasing the source of the uric acid,reducing uric acid excretion and inhibiting uricase were used.We observed the influence on the serum uric acid and other indicators of rats induced by some factors:the lipid emulsion,high purine diet,beer with sugar,beer with sugar and high purine,and so on.Then we choose one of the stable and sustained animal models,studying the effects of Plo.E(which extracted from a TCM)on modulating the level of serum uric acid and the preliminary mechanism in this abstract.RESULTS ① At the 2nd week,the level of serum UA,BUN,Cr,TC,LDL-c of rats in the lipid emulsion group raised significantly.② At the 6th weeks,the serum UA in both the high purine diet group and lipid emulsion group raised obviously.③ The effects of Plo.E on hyperuricemia rats induced by high purine diet:After 8d administration,the Plo.E(three dosages)can reduce the UA level,and the middle and low dosages can reduce the TG level.After 25 dadministration,Plo.E can reduce the plasma viscosity,UA,TG,the whole blood viscosity level,the high dosage also can reduce the TC level.CONCLUSION The rats induced by the high purine diet and lipid emulsion can raised the serum UA obviously,while the way of lipid emulsion is earlier and more stable.Plo.E has a therapeutic effect on hyperuricemia,with an activity of reducing plasma viscosity and blood lipid.The above models conform to the pathogenesis of humans,can be used to study the causes and pathogenesis of hyperuricemia complicating metabolic disorder and the related treatment drug screening.

Combined anti-hyperuricemia effect of Di.E and Gpm.E on hyperuricemia rats induced by high-purine diet

OBJECTIVE Di.E and Gpm.E were separately extracted from two kinds of traditional Chinese medicine.The aim of this research is to investigate the combined anti-hyperuricemia effect of Di.E and Gpm.E on hyperuricemia rats induced by high-purine diet,and to study the underlying mechanism.METHODS Sprague Dawley(SD)rats were divided into some groups according to their serum uric acid(UA),the normal control group received standard diet,while others were fed with high-purine diet.After the success of modeling,SD rats were divided into 5groups according to UA:normal control group,model control group,Di.E and Gpm.E(high,middle,low dosages).The treatment groups were simultaneously orally administered.Two days before the last administration,the urine N-acetyl beta-D Glucosaminidase(NAG),Lysozyme(LYS)activity were separately detected using the urine collected from metabolic cage for 24 h.After the last administration,the blood was taken from postcava to detect the level:① The related indexes of liver and kidney function:the serum UA,blood urea nitrogen(BUN),creatinine(CR),alanine aminotransferase(ALT);② The uric acid metabolism related enzymes:the serum and liver xanthine oxidase(XOD),adenosine deaminase(ADA),guanine deaminase(GD),xanthine dehydrogenase(XDH)activity;③ Fibrosis related indexes of renal tissue:connective tissue growth factor(CTGF),monocyte chemotactic protein-1(MCP-1),TGF-β1,TNF-α,NF-κB levels.RESULTS Di.E and Gpm.E(high,middle dosages)could markedly reduce the serum UA level significantly,also can decrease XOD,ADA,TNF-α,MCP-1,NF-κB activity significantly.CONCLUSION The results show that Di.E and Gpm.E can decrease the serum UA level significantly,improve the liver and kidney function and slow down the process of renal fibrosis;the therapeutic effects may be related to their inhibition of XOD,ADA activity.