基于超高效液相色谱-质谱联用的非靶向代谢组学方法探究不同年龄组驼肉代谢物的差异及变化规律。结果表明,在3个年龄组(3~4、6~7岁和9~10岁,分别以I、II和III组表示)骆驼背最长肌中共鉴定出显著差异代谢物710种;在I vs II组显著差异代...基于超高效液相色谱-质谱联用的非靶向代谢组学方法探究不同年龄组驼肉代谢物的差异及变化规律。结果表明,在3个年龄组(3~4、6~7岁和9~10岁,分别以I、II和III组表示)骆驼背最长肌中共鉴定出显著差异代谢物710种;在I vs II组显著差异代谢物有78个,其中I组上调47个,II组上调31个;在II vs III组显著差异代谢物有49个,其中II组上调18个,III组上调31个;在I vs III组显著差异代谢物有65个,其中I组上调29个,III组上调36个。京都基因与基因组百科全书通路分析结果表明,差异代谢物主要富集到蛋白质和氨基酸代谢、脂肪酸、维生素和矿物质代谢等相关通路,说明不同生长阶段骆驼中各营养素的消化代谢均有差异。I组中的多不饱和脂肪酸(polyunsaturated fatty acid,PUFA)(尤其n-6 PUFA、n-3 PUFA)含量和PUFA/不饱和脂肪酸值均显著高于III组,这主要与相关代谢通路上的花生四烯酸、亚油酸和13-L-过氧化氢油酸浓度的显著上调有关;同时L-亮氨酸、L-缬氨酸、L-谷氨酰胺等差异代谢物可以作为不同年龄驼肉品质差异的潜在标记物。展开更多
食品安全已成为一个重要的公共卫生问题,快速、准确地监测和检测食源性致病菌是控制和预防人类食源性疾病的最有效方法之一。由于食品基质的复杂性、细菌的多样性及不同生长和复制特性,给食源性致病菌检测带来了重大挑战。传统微生物检...食品安全已成为一个重要的公共卫生问题,快速、准确地监测和检测食源性致病菌是控制和预防人类食源性疾病的最有效方法之一。由于食品基质的复杂性、细菌的多样性及不同生长和复制特性,给食源性致病菌检测带来了重大挑战。传统微生物检测方法耗时费力,不足以满足不可培养活菌细胞和现场快速食品检测的要求。因此,近年来针对食源性致病菌开发了各种免疫检测技术,比传统方法更加灵敏、简单和高效,具有广阔的应用前景。该文结合食源性致病菌亚致死损伤、活的不可培养(viable but non-culturable,VBNC)和休眠3种代谢状态的生物学特征及抗体的类型和特点,综述了当前用于食源性致病菌常见的免疫技术的检测原理、优缺点和应用,并对现有方法的局限性和未来发展方向进行讨论,以期为食源性致病菌免疫检测技术的开发和利用提供参考。展开更多
AIM:To assess the efficacy of artificial natural light in preventing incident myopia in primary school-age children.METHODS:This is a prospective,randomized control,intervention study.A total of 1840 students from 39 ...AIM:To assess the efficacy of artificial natural light in preventing incident myopia in primary school-age children.METHODS:This is a prospective,randomized control,intervention study.A total of 1840 students from 39 classes in 4 primary schools in Foshan participated in this study.The whole randomization method was adopted to include classes as a group according to 1:1 randomized control.Classrooms in the control group were illuminated by usual light,and classrooms in the intervention group were illuminated by artificial natural light.All students received uncorrected visual acuity and best-corrected visual acuity measurement,non-cycloplegic autorefraction,ocular biometric examination,slit lamp and strabismus examination.Three-year follow-up,the students underwent same procedures.Myopia was defined as spherical equivalent refraction≤-0.50 D and uncorrected visual acuity<20/20.RESULTS:There were 894 students in the control group and 946 students in the intervention group with a mean±SD age of 7.50±0.53y.The three-year cumulative incidence rate of myopia was 26.4%(207 incident cases among 784 eligible participants at baseline)in the control group and 21.2%(164 incident cases among 774 eligible participants at baseline)in the intervention group[difference of 5.2%(95%CI,3.7%to 10.1%);P=0.035].There was also a significant difference in the three-year change in spherical equivalent refraction for the control group(-0.81 D)compared with the intervention group[-0.63 D;difference of 0.18 D(95%CI,0.08 to 0.28 D);P<0.001].Elongation of axial length was significantly different between in the control group(0.77 mm)and the intervention group[0.72 mm;difference of 0.05 mm(95%CI,0.01 to 0.09 mm);P=0.003].CONCLUSION:Artificial natural light in the classroom of primary schools can result in reducing incidence rate of myopia during a period of three years.展开更多
Polyoxymethylene methacrylate (PMMA) is widely used in ophthalmic biomaterials. Misuse of PMMA in extreme environments is likely to damage the ocular surface and intraocular structures. The surface characterization an...Polyoxymethylene methacrylate (PMMA) is widely used in ophthalmic biomaterials. Misuse of PMMA in extreme environments is likely to damage the ocular surface and intraocular structures. The surface characterization and tribological behavior of PMMA processed using an excimer laser were investigated in this study by contrasting diferent lubrication conditions and friction cycles. The results show that the roughness of the material surface increases with laser processing, which changes its physical structure. Under lubrication, the laser-treated PMMA exhibits better hydrophilicity, especially during the use of eye drops. No obvious relationship exists between the laser-processing time and friction behavior. However, the laser treatment may contribute to the formation of friction and wear mechanisms of PMMA materials. Laser-treated PMMA in saline solution exhibits better abrasive resistance by showing a lower wear rate than that in eye drops, although it has a higher friction coefcient. In this study, the diferent friction stages in laser-treated PMMA were clarifed under two lubrication conditions. The wear rates of the laser-treated PMMA were found to decrease with the number of cycles, and the friction coefcient has a similar variation tendency. The wear behavior of the laser-treated PMMA is dominated by the main abrasive wear and secondary transferred flm formation. This study provides a theoretical basis for the development and application of ophthalmic biomaterials in complex environments by examining the material surface interface behavior and wear mechanism after laser processing using PMMA as the research matrix.展开更多
文摘基于超高效液相色谱-质谱联用的非靶向代谢组学方法探究不同年龄组驼肉代谢物的差异及变化规律。结果表明,在3个年龄组(3~4、6~7岁和9~10岁,分别以I、II和III组表示)骆驼背最长肌中共鉴定出显著差异代谢物710种;在I vs II组显著差异代谢物有78个,其中I组上调47个,II组上调31个;在II vs III组显著差异代谢物有49个,其中II组上调18个,III组上调31个;在I vs III组显著差异代谢物有65个,其中I组上调29个,III组上调36个。京都基因与基因组百科全书通路分析结果表明,差异代谢物主要富集到蛋白质和氨基酸代谢、脂肪酸、维生素和矿物质代谢等相关通路,说明不同生长阶段骆驼中各营养素的消化代谢均有差异。I组中的多不饱和脂肪酸(polyunsaturated fatty acid,PUFA)(尤其n-6 PUFA、n-3 PUFA)含量和PUFA/不饱和脂肪酸值均显著高于III组,这主要与相关代谢通路上的花生四烯酸、亚油酸和13-L-过氧化氢油酸浓度的显著上调有关;同时L-亮氨酸、L-缬氨酸、L-谷氨酰胺等差异代谢物可以作为不同年龄驼肉品质差异的潜在标记物。
文摘食品安全已成为一个重要的公共卫生问题,快速、准确地监测和检测食源性致病菌是控制和预防人类食源性疾病的最有效方法之一。由于食品基质的复杂性、细菌的多样性及不同生长和复制特性,给食源性致病菌检测带来了重大挑战。传统微生物检测方法耗时费力,不足以满足不可培养活菌细胞和现场快速食品检测的要求。因此,近年来针对食源性致病菌开发了各种免疫检测技术,比传统方法更加灵敏、简单和高效,具有广阔的应用前景。该文结合食源性致病菌亚致死损伤、活的不可培养(viable but non-culturable,VBNC)和休眠3种代谢状态的生物学特征及抗体的类型和特点,综述了当前用于食源性致病菌常见的免疫技术的检测原理、优缺点和应用,并对现有方法的局限性和未来发展方向进行讨论,以期为食源性致病菌免疫检测技术的开发和利用提供参考。
基金Guangdong Basic and Applied Basic Research Foundation(No.2019B1515120011)Medical Research,Foshan Health and Wellness Department(No.20220374).
文摘AIM:To assess the efficacy of artificial natural light in preventing incident myopia in primary school-age children.METHODS:This is a prospective,randomized control,intervention study.A total of 1840 students from 39 classes in 4 primary schools in Foshan participated in this study.The whole randomization method was adopted to include classes as a group according to 1:1 randomized control.Classrooms in the control group were illuminated by usual light,and classrooms in the intervention group were illuminated by artificial natural light.All students received uncorrected visual acuity and best-corrected visual acuity measurement,non-cycloplegic autorefraction,ocular biometric examination,slit lamp and strabismus examination.Three-year follow-up,the students underwent same procedures.Myopia was defined as spherical equivalent refraction≤-0.50 D and uncorrected visual acuity<20/20.RESULTS:There were 894 students in the control group and 946 students in the intervention group with a mean±SD age of 7.50±0.53y.The three-year cumulative incidence rate of myopia was 26.4%(207 incident cases among 784 eligible participants at baseline)in the control group and 21.2%(164 incident cases among 774 eligible participants at baseline)in the intervention group[difference of 5.2%(95%CI,3.7%to 10.1%);P=0.035].There was also a significant difference in the three-year change in spherical equivalent refraction for the control group(-0.81 D)compared with the intervention group[-0.63 D;difference of 0.18 D(95%CI,0.08 to 0.28 D);P<0.001].Elongation of axial length was significantly different between in the control group(0.77 mm)and the intervention group[0.72 mm;difference of 0.05 mm(95%CI,0.01 to 0.09 mm);P=0.003].CONCLUSION:Artificial natural light in the classroom of primary schools can result in reducing incidence rate of myopia during a period of three years.
基金Supported by the Chengdu Municipal Medical Research Project(Grant Nos.2019005 and 2022014)Sichuan Provincial Cadres Health Research Project(Grant No.ChuanGanYan(2013-104)).
文摘Polyoxymethylene methacrylate (PMMA) is widely used in ophthalmic biomaterials. Misuse of PMMA in extreme environments is likely to damage the ocular surface and intraocular structures. The surface characterization and tribological behavior of PMMA processed using an excimer laser were investigated in this study by contrasting diferent lubrication conditions and friction cycles. The results show that the roughness of the material surface increases with laser processing, which changes its physical structure. Under lubrication, the laser-treated PMMA exhibits better hydrophilicity, especially during the use of eye drops. No obvious relationship exists between the laser-processing time and friction behavior. However, the laser treatment may contribute to the formation of friction and wear mechanisms of PMMA materials. Laser-treated PMMA in saline solution exhibits better abrasive resistance by showing a lower wear rate than that in eye drops, although it has a higher friction coefcient. In this study, the diferent friction stages in laser-treated PMMA were clarifed under two lubrication conditions. The wear rates of the laser-treated PMMA were found to decrease with the number of cycles, and the friction coefcient has a similar variation tendency. The wear behavior of the laser-treated PMMA is dominated by the main abrasive wear and secondary transferred flm formation. This study provides a theoretical basis for the development and application of ophthalmic biomaterials in complex environments by examining the material surface interface behavior and wear mechanism after laser processing using PMMA as the research matrix.