LncRNA MEG3 Inhibits the Epithelial-mesenchymal Transition of Bladder Cancer Cells through the Snail/E-cadherin Axis

Objective This study aimed to investigate the role of the long noncoding RNA(lncRNA)maternally expressed gene 3(MEG3)in the epithelial-mesenchymal transition(EMT)of bladder cancer cells and the potential mechanisms.Methods Cell invasion,migration,and wound healing assays were conducted to assess the effects of MEG3 on the invasive and migratory capabilities of bladder cancer cells.The expression levels of E-cadherin were measured using Western blotting,RT-qPCR,and dual luciferase reporter assays.RNA immunoprecipitation and pull-down assays were performed to investigate the interactions between MEG3 and its downstream targets.Results MEG3 suppressed the invasion and migration of bladder cancer cells and modulated the transcription of E-cadherin.The binding of MEG3 to the zinc finger region of the transcription factor Snail prevented its ability to transcriptionally repress E-cadherin.Additionally,MEG3 suppressed the phosphorylation of extracellular regulated protein kinase(ERK),c-Jun N-terminal kinase(JNK),and P38,thereby decreasing the expression of Snail and stimulating the expression of E-cadherin.Conclusion MEG3 plays a vital role in suppressing the EMT in bladder cancer cells,indicating its potential as a promising therapeutic target for the treatment of bladder cancer.

Physical-Rheological Properties and Performances of Rejuvenated(Styrene-Butadiene-Styrene)Asphalt with Polymerized-MDI and Aromatic Oil

Traditional asphalt rejuvenators,like aromatic oil(AO),are known to be effective in improving the low-temperature properties and fatigue performances of aged SBS(styrene-butadiene-styrene)modified asphalt(SBSMA)binders and mixtures.However,these rejuvenators inevitably compromise their high-temperature properties and deformation resistances because they dilute asphalt binder but do not fix the damaged structures of aged SBS.In this study,a highly-active chemical called polymerized 4,4-diphenylmethane diisocyanate(PMDI)was used to assist the traditional AO asphalt rejuvenator.The physical and rheological characteristics of rejuvenated SBSMA binders and the moisture-induced damage and rut deformation performances of corresponding mixtures were comparatively evaluated.The results showed that the increasing proportion of AO compromises the hightemperature property and hardness of aged SBSMA binder,and an appropriate amount of PMDI works to compensate such losses;3%rejuvenator at mass ratio of AO:PMDI=70:30 can have a rejuvenated SBSMA binder with a high-temperature performance similar to that of fresh binder,approximately at 71.4°C;the use of AO can help reduce the viscosity of PMDI rejuvenated SBSMA binder for improving its workability;PMDI can help improve the resistance of AO rejuvenated SBSMA binder to deformation,especially at elevated temperatures,through its chemical reactions with aged SBS;moisture induction can enhance the resistance to damage of rejuvenated mixtures containing AO/PMDI or only PMDI;and the rejuvenator with a mass ratio of AO:PMDI=70:30 can lead the rejuvenated mixture to meet the application requirement,with a rut depth of only 2.973 mm,although more PMDI can result in a higher resistance of rejuvenated mixtures to high-temperature deformation.

Performance Characterization of CR/PU Asphalt for Potential Application in Assembled Fast-Repairing Engineering

Conventional repairing methods for asphalt pavement have some inconveniences,such as insufficient strength,and are typically time-consuming.To address these issues,this study proposes a new technological method to design and prepare a high-performance assembled asphalt concrete block for fast repair of the potholes.A series of composite modified asphalt binders with 10%crumb rubber(CR)and different dosages(0%,1%,3%,5%)of polyurethane(PU)are examined to determine the optimized binder.Subsequently,the corresponding asphalt mixtures are prepared for further comparison and assessment of engineering properties,such as moistureinduced damage,high-temperature deformation,and low-temperature cracking characteristics.The test results show that PU can significantly improve the high-temperature performance and hardness of(crumb rubber modified asphalt)CRMA binder;3%PU contributes allowing the resistance of CRMA mixture to moisture-induced damage at higher levels,particularly under water whole immersion;as 3%PU is added,the high-temperature rutting deformation resistance of the CRMA mixture increases significantly,and the low-temperature anti-cracking properties are also improved slightly.Therefore,the innovatively designed high-quality assembled fast-repairing asphalt concrete block is recommended as an appropriate option for highway maintenance.

Management of a Retained Epidural Catheter in a Pregnant Patient: A Case Report and Review of Literature

Background: Epidural anesthesia is an effective intervention to treat labor pain and provide analgesia for orthopedic procedures. A rare complication of this technique is epidural catheter retention. Case Presentation: In this case report, we present an otherwise healthy 21-year-old G2P0010 woman at 39 weeks and 3 days gestation whose anesthetic management was complicated by a symptomatic retained epidural catheter fragment. Computed tomography (CT) imaging of her spine showed the fractured catheter within her paraspinal muscles and neurosurgery recommended no surgical intervention at that time. The patient reported resolution of back pain while in the postpartum unit which she continues to endorse two weeks after discharge. Conclusion: Retained epidural catheter is a medical emergency that requires immediate imaging and neurological evaluation. While magnetic resonance imaging is typically regarded as the standard imaging modality for this complication, in epidural sets that contain metallic components, we suggest using CT to avoid the risk of thermal nerve damage. In cases where the patient does not report any acute complaints, nonsurgical management may be appropriate but close follow-up is required to monitor for catheter migration.

交错群A_(4)在布尔系统上的不可表示性

非非悖论是一类比较典型的具有对称性的语义悖论,这类悖论的对称性都可用置换群进行刻画。当一个置换群刻画了某个悖论的对称性时,我们称此置换群可由该悖论表示。Hisung(2022)提出如下猜测:任意置换群都可由非非类型的悖论表示。本文通过使用群映射等概念来分析群作用到真值序列集上的轨道和稳定化子,证明了交错群A4不能被任何布尔系统表示。这表明如果把非非类型的悖论限制在布尔悖论的范围内,并不是每个置换群都能由非非类型悖论来表示。本文还通过置换群直和构造给出了其他不能被布尔系统表示的置换群例子。研究表明不可被布尔系统表示的置换群不是孤立的,而是有一定存在基础的。

Anesthetic Management of a Patient with Spinal Muscular Atrophy Type III Undergoing Emergent Caesarean Section: A Case Report

In this case report, we describe the anesthetic management for a 36-year-old G2P0010 at 36 weeks gestation with Spinal Muscular Atrophy Type III who underwent an emergent caesarean section due to fetal footling breech position. The patient is a wheelchair-bound quadriplegic with kyphoscoliosis and a lack of cough reflex who required nasal continuous noninvasive ventilatory support (CNVS) for chronic hypercapnic respiratory failure. Surgery was done under general anesthesia due to its emergent nature, and the patient was successfully extubated and transitioned to nasal CNVS in the operating room at the end of the case. Postoperative care was provided in the medical intensive care unit for three days without complication and the patient was discharged home uneventfully.

Worldwide research trends on chloroquine:a bibliometric analysis from 2012 to 2021

Background:Chloroquine(CQ)is an antimalarial drug that was first synthesized by Hans Andersag,a German scientist,in 1934.Chloroquine has been widely researched and used over the years.The rapid development in the fields of modern science and technology has also contributed to the increase in interest in chloroquine.Hence,it is necessary to comprehensively summarize the research trends to understand the breakthroughs made in the field.Methods:The required data was compiled by analyzing the Web of Science Core Collection(WoSCC)database.The search period for studying global research trends in chloroquine research was set from 2012 to 2021 to ensure a comprehensive analysis over an extended timeframe.Data retrieval was performed on April 4,2022,focusing on articles and reviews published in English.The retrieval words were:(TS=(chloroquine))OR(TS=(aralen)).A total of 1,091 reviews and 7,259 articles were retrieved and analyzed.The data obtained from WoSCC was captured and analyzed using VOS viewer(version 1.6.16)and Citesspace(version 5.8.R5).Results:The number of literature reports on chloroquine published in the past 10 years has shown an annual increase.Among the countries,the United States has contributed the highest number of papers and ranks first in terms of both H-index and citation count.The League of European Research Universities is one of the largest research-focused university networks,and Malaria Journal stands out as a prominent journal publishing articles relevant to the field of study.A paper authored by Gautret and Philippe in 2020 achieved the highest citation score globally.The biosynthesis of chloroquine,mechanisms of drug resistance,and drug combinations are receiving growing attention.Conclusion:The research area of chloroquine has been significantly influenced by the American and China.The progress of chloroquine research is further propelled by fruitful collaborations among various countries.Researchers have extensively studied the anti-malarial effect,drug resistance mechanism,and autophagy of chloroquine for the development of a treatment method for COVID-19 based on chloroquine.Bibliometric analysis can be employed to identify hotspots,new directions,and frontiers in the field of chloroquine research.

Is It Safe to Perform an Autologous Epidural Blood Patch on Patients with Underlying Spinal Stenosis or Lumbar Disc Disease? Case Report and Literature Review

The most common spinal pathology seen in the obstetric population is lumbar disc herniation. There is currently no literature documenting the safety of performing an epidural blood patch on obstetric patients with underlying spinal pathology. We present a case of a patient with known severe lumbar spinal stenosis with compressive radiculopathy who received a successful epidural blood patch without worsening her underlying neurologic symptoms. Epidural blood patches can be safely performed in this patient population. However, the anesthesiologist should be aware of the risk of potentially worsening preexisting neurological deficits. Thus, we advise caution prior to placing an epidural blood patch on these patients. The risks and benefits of the procedure should be carefully weighed and considered. It is important to have a thorough discussion with the patient regarding the risks of an epidural blood patch prior to performing the procedure.

Anesthetic and Obstetric Considerations of Moyamoya Disease in Pregnancy after Intracranial Bypass Grafting: A Case Report and Literature Review

Moyamoya disease (MD) is a chronic idiopathic vasculopathy characterized by bilateral stenosis of the distal internal carotid arteries (ICA’s) with subsequent formation of abnormal collateral vessels. The underlying etiology is unknown [1]. Patients with MD are predisposed to intracranial hemorrhage or cerebral ischemia given the fragile nature of the collateral circulation. Although there is no curative treatment for MD, there are surgical palliative procedures that can augment cerebral blood flow to areas of impaired perfusion and circumvent the abnormal collateral circulation. Medical management exists for patients who are not surgical candidates. Because the incidence of MD peaks during the second and third decades of life, it can potentially affect women of childbearing age [2]. However, the optimal anesthetic and obstetric management of the parturient with MD remains controversial. We present a case of a primigravida with a history of MD who underwent intracranial bypass grafting prior to conception and subsequently had a successful cesarean delivery that was complicated by transient ischemic attacks (TIA) in the postpartum period.

A Validation Pilot Study Comparing Telemedicine Images to a Face-to-Face Airway Exam for Conducting the Anesthesia Preoperative Airway Evaluation

Telemedicine is a branch of healthcare that uses communication technology to deliver medical information and services between patients and healthcare providers. The applicability of telemedicine is vast and increasingly relevant. There is a lack of research on utilizing telemedicine for remote evaluation of the airway. The primary aim of this pilot study was to validate a telemedical airway exam as a viable alternative to an in-person evaluation. Three anesthesiologists evaluated 48 volunteers by telemedicine and live examination. The telemedical exam consisted of transmitting still images of four established, predictive parameters of difficult airways: Mallampati score;neck extension;ability to prognath;and thyromental distance. Each subject’s telemedical and face-to-face scores were compared to determine their degree of correlation. Still images were taken using standardized positioning with four pictures of mouth opening, neck extension, prognath, and thyromental distance. Data were analyzed using Wilcoxon signed-rank tests and free-marginal multirater kappa analysis. Average respective scores for live versus telemedicine examination were as follows: Mallampati scores were 1.73 versus 2.54;neck extension scores were 3.77 versus 3.60;thyromental distance (measured in finger breadths) was 2.95 versus 2.92;and prognath scores were 0.97 versus 0.94. There was no difference in grading of thyromental distance or prognathy ability between live and telemedical exams, and interrater reliability was very good for both parameters. This study supports telemedicine as a reliable tool for preoperative anesthesia airway exams to identify airway difficulties. This may be especially useful as an alternative in patients with COVID-19 undergoing urgent surgery.

Monitoring disease progression and treatment efficacy with circulating tumor cells in esophageal squamous cell carcinoma: A case report

This study investigated whether changes in circulating tumor cell(CTC) numbers reflect tumor progression and treatment efficacy in esophageal squamous cell carcinoma(ESCC). A 47-year-old male patient with ESCC is presented in this case study. The patient was evaluated for a series of serum tumor markers and subjected to radiological examinations before and after surgery and during follow-up over the course of five years. In addition, the CTCs in 7.5 m L of peripheral blood were enriched by magneticactivated cell sorting negative selection and identified by immunofluorescence staining. Serum tumor markers remained within normal ranges and were discordant with imaging scans during the follow-up. Initially, one CTC was detected in the peripheral blood sample, and 14 were observed seven days after the operation. After 12 wk, subcutaneous metastases and bone metastases occurred, and the number of CTCs increased to 84. After 48 wk, lung metastases were noted, and the CTC level was 21. At 104 wk, the number of CTCs was 14, and disease recurrence was detected by positron emission tomography-computed tomography. The CTC counts were in accord with the imaging studies at several time points. The additional information provided by CTC enumeration could thus facilitate monitoring of disease status and treatment efficacy and provide support for treatment decisions.

Blockade of Brain γ-Aminobutyric Acid a Receptors Antagonizes Hypnotic Action of Isoflurane in Rats―GABA Receptor and Isoflurane Induced Hypnosis

Although in vitro studies have demonstrated that isoflurane potentiates the function of γ-aminobutyric acid A receptors (GABAARs), the in vivo data are controversial. To determine if GABAARs contribute to the loss-of righting reflex (LORR) induced by isoflurane, we studied the LORR in the absence and presence of gabazine, a competitive GABAAR antagonist, in Sprague-Dawley rats anesthetized with either isoflurane or ketamine. Administration of isoflurane and ketamine induced LORR in a dose-dependent manner. Gabazine significantly antagonized the effect of isoflurane and shifted the dose response curve to the right. In addition, gabazine prolonged the onset time of LORR induced by isoflurane. Ketamine induced LORR was not affected by gabazine. This indicates that centrally administered gabazine selectively blocks the effect of isoflurane, and the effect of gabazine is not due to a non-specific CNS excitatory action. These results suggest that the hypnotic effect of isoflurane is at least in part mediated by GABAARs.

长江沱沱河源区水温变化规律分析

本文基于长江源沱沱河水文站1977-2015年月实测水温资料,对高于0℃的5-10月水温年际变化特征进行了分析。结果表明：沱沱河水文站高于0℃以上累积水温增高趋势明显,2011-2015年较1977-1980年年平均累积值升高189.6℃,日平均升高1.2℃,但每年水温高于0℃的天数却无明显变化趋势,平均每年161天;5-9月水温均有明显的升温,2011-2015年较1977-1980年分别升高0.6℃、1.6℃、1.7℃、1.1℃、1.0℃;各月水温趋势突变点发生在2005-2007年之间,多数月份发生在2005年和2006年。6-9月存在明显的28年时间尺度的主周期,5月和10月因水温较低且接近0℃,周期特性不明显。

Correct a Wide Spread Conclusion of Cantor Set Theory

The Cantor’s conclusion that “there is a one-one correspondence between the points on n-dimensional space and the points on the line” appears in the numerous current documents. By introducing a monotonic and continuous function, a one-one correspondence between two intervals is built;and by using parametric equations of the curve, a one-one correspondence from the points on the curve to the points on the line is established. Specially, the meanings of multivariate functions are given. By using a n-variable equation with a parameter, a correspondence from n-dimensional space area to a interval is built, so the wrong conclusion is completely denied. The paper enriches calculus and can reduce the teaching difficulty of real function in some degree. The expression of moving curve (surface) limit is given in the paper. More importantly, after the conclusion is corrected, it will be necessary and possible to re-establish the theory and the approach about multivariable differential calculus.

Method for Single Intravenous Anesthetic Infusion in a Rodent Model

Venous catheterization, endotracheal intubation, and mechanical ventilation are necessary for performing total intravenous general anesthesia in rats. Intubation and IV cannulation of the rat is challenging because of the animals’ small size and the lack of equipment specifically designed for the restricted anatomical dimensions. Here, we present methods for tail venous catheterization and intubation that are quickly learned by the provider with clinical operating room experience but lack experience in the lab. For tail venous catheterization, each rat (n = 20) was gently restrained in a rat chamber;its tail was placed in warm water for 10 minutes, and a 24 gauge intravenous catheter was inserted into the lateral tail vein. The catheter was fixed in place using tape and attached to a T-connector for drug administration. A bolus of propofol (n = 10), ketamine (n = 7), or etomidate (n = 3) was administered to achieve rapid deep anesthesia. Once anesthetized, rats were intubated with the aid of a modified pediatric laryngoscope. The standard miller blade 0 was cut on each side for approximately 2/3 of the total blade length to remove a total of half the width. After the ventilator was properly set, the rats’ vital signs and metabolic status were monitored. Throughout the one-hour infusion, the rats’ physiologic parameters were maintained within normal range. These results indicate that intravenous general anesthesia can be performed effectively and safely in small animals using the refined catheterization and intubation methods tested in this study. These techniques are easily reproducible and learned as they mimic the tools and strategies commonly used in the OR.

Anesthesia Considerations in Emergency C-Section and Perioperative Neonatal Resuscitation: A Case Report and Review of Literature

Emergency C-sections are performed for various reasons. This is a case report of emergency C-section performed due to fetal distress. This is a classic case report which emphasizes the importance of managing emergency C-sections according to international standards and acknowledges co-operation of obstetrician and anesthesiologist. We reviewed literature about emergency C-sections and discussed sensitive time intervals, types of anesthesia and neonatal resuscitation.

太阳极轨天文台

太阳极轨天文台利用高轨道倾角(≥80°)和小椭偏率轨道,将首次实现正面对太阳极区磁场和速度场高精度成像观测,结合多波段遥感和原位测量,为解决太阳磁活动周起源的世纪难题提供决定性观测,为破解“原始”高速太阳风的起源效应之谜提供直接观测支撑,为理解日球层整体结构突破性创建数据驱动的日球数值模型.

太阳立体探测任务设想

太阳空间探测在太阳物理前沿科学问题研究和空间天气预报应用研究方面具有重要的意义.人类在60余年的太阳空间探测活动中,取得了一系列重要成果,但仍存在诸多根本性重大问题有待解决.太阳立体探测可以克服单视角观测的局限,获取全方位、多要素的物理数据,促进解决太阳物理中的重大科学问题.本文介绍了太阳内部结构和磁场起源、太阳活动机理研究、太阳活动的全日球空间天气效应和空间天气预报模式研究4个科学目标,太阳立体探测任务空间布局、系统组成和探测器总体设计,以及太阳立体探测任务的有效载荷配置和主要技术指标.