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分枝杆菌HGMS2中类固醇C20-羟基脱氢酶的鉴定
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作者 明卓 胡青 +1 位作者 宋士奎 苏正定 《工业微生物》 CAS 2023年第4期170-178,共9页
分枝杆菌常被用作甾体药物中间体生产菌种,然而当前人们对其具体的甾醇降解机制仍然不是很清楚。为了获得C20-羟基甾药中间体,文章直接以RS为底物进行了转化,并通过对转化产物进行TLC、HPLC、LS-MS和核磁分析,初步确定分枝杆菌中存在类... 分枝杆菌常被用作甾体药物中间体生产菌种,然而当前人们对其具体的甾醇降解机制仍然不是很清楚。为了获得C20-羟基甾药中间体,文章直接以RS为底物进行了转化,并通过对转化产物进行TLC、HPLC、LS-MS和核磁分析,初步确定分枝杆菌中存在类固醇C20-羟基脱氢酶(1DHC)参与的代谢途径。同时,基于生物信息学和结构生物学,通过序列和结构比对分析,最终从分枝杆菌中鉴定出了一个与类固醇C20-羟基脱氢酶同源性很高的基因。将该基因在大肠杆菌中异源表达,并对其功能活性进行分析,证明该基因所编码的酶和类固醇C20-羟基脱氢酶具有相同的功能活性。文章首次从分枝杆菌中鉴定出一种类固醇C20-羟基脱氢酶,使研究者对分枝杆菌甾醇代谢机制有了更深入的理解,同时也为新型甾药中间体的制备以及分枝杆菌的改造奠定了理论基础。 展开更多
关键词 分枝杆菌 类固醇C20β-羟基脱氢酶 产物分析 17α-羟基-孕甾-4-烯-3 20-二酮
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甲壳素-硅酸四乙酯膜的制备和性能 被引量:3
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作者 吕恺 卓鸣 +3 位作者 张颖 张雪艳 陆茵 史西志 《高分子材料科学与工程》 EI CAS CSCD 北大核心 2019年第11期155-160,共6页
采用N,N-二甲基乙酰胺(DMAC)和无水氯化锂(LiCl)溶解甲壳素,以丙酮为非溶剂通过液致相分离法制备甲壳素膜,利用硅酸四乙酯对甲壳素膜进行改性,选用扫描电子显微镜、能谱仪、傅里叶变换红外光谱仪对膜性能进行表征,并研究了甲壳素-硅酸... 采用N,N-二甲基乙酰胺(DMAC)和无水氯化锂(LiCl)溶解甲壳素,以丙酮为非溶剂通过液致相分离法制备甲壳素膜,利用硅酸四乙酯对甲壳素膜进行改性,选用扫描电子显微镜、能谱仪、傅里叶变换红外光谱仪对膜性能进行表征,并研究了甲壳素-硅酸四乙酯膜对牛血清蛋白(BSA)的过滤特性。结果表明, 5%LiCl/DMAC溶剂可以快速溶解甲壳素,纯甲壳素膜的收缩程度非常剧烈,而甲壳素-硅酸四乙酯膜收缩程度较小并具有较好的拉伸强度,对BSA有一定的截留效果,铸膜液中甲壳素的浓度对BSA的截留率有较大影响。当铸膜液中甲壳素浓度从1%增加到1.8%时,对BSA的截留率从23.6%增加到了75%。 展开更多
关键词 甲壳素 硅酸四乙酯 过滤
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新月弯孢霉转录组分析及甾体C_(11)β-羟化酶基因鉴定
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作者 明卓 宋士奎 苏正定 《生物技术》 CAS 2023年第5期537-543,共7页
[目的]分析新月弯孢霉转录组,对甾体C_(11)β-羟基化酶基因进行鉴定。[方法]结合生物信息学和蛋白结构学对转录组分析,利用AlphaFold2进行蛋白结构模拟,与已知人源醛固酮合酶hCYP11B 2进行结构比对。[结果]在转录组中,共获得24943条Unig... [目的]分析新月弯孢霉转录组,对甾体C_(11)β-羟基化酶基因进行鉴定。[方法]结合生物信息学和蛋白结构学对转录组分析,利用AlphaFold2进行蛋白结构模拟,与已知人源醛固酮合酶hCYP11B 2进行结构比对。[结果]在转录组中,共获得24943条Unigenes,平均长度1915 bp。18792条(75.34%)可以匹配到蛋白数据库获得注释信息。Unigenes在各数据库中注释的基因数分别为:在COG数据库中有5615条(22.51%),在GO数据库中有4424条(17.74%),在KEGG数据库中有6322条(25.35%),在Swiss-Prot数据库中有11348条(45.50%)。通过初筛获得39个基因。经过AlphaFold2的结构模拟,与人的甾体醛固酮合酶(hCYP11B2)结构比对进行复筛,共26条甾体C_(11)β-羟基化酶相关基因被鉴定出来。[结论]该新月弯孢霉菌株具有甾体C_(11)β-羟基化的能力,通过对其转录组进行分析,共鉴定出26条甾体C_(11)β-羟基化酶相关基因。 展开更多
关键词 新月弯孢霉 甾体C_(11)β-羟基化酶 转录组 结构比对 生物信息学分析
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0.04 degree-per-hour MEMS disk resonator gyroscope with high-quality factor (510 k) and long decaying time constant(74.9 s) 被引量:17
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作者 Qingsong Li Dingbang Xiao +6 位作者 Xin Zhou Yi Xu ming zhuo Zhanqiang Hou Kaixuan He Yongmeng Zhang Xuezhong Wu 《Microsystems & Nanoengineering》 EI CSCD 2018年第1期46-56,共11页
The disk resonator gyroscope is an attractive candidate for high-performance MEMS gyroscopes.This gyroscope consists of a sensor and readout electronics,and the characteristics of the sensor directly determine the per... The disk resonator gyroscope is an attractive candidate for high-performance MEMS gyroscopes.This gyroscope consists of a sensor and readout electronics,and the characteristics of the sensor directly determine the performance.For the sensor,a high-quality factor and long decaying time constant are the most important characteristics required to achieve high performance.We report a disk resonator gyroscope with a measured quality factor of 510 k and decaying time constant of 74.9 s,which is a record for MEMS silicon disk resonator gyroscopes,to the best of our knowledge.To improve the quality factor of the DRG,the quality factor improvement mechanism is first analyzed,and based on this mechanism two stiffness-mass decoupled methods,i.e.,spoke length distribution optimization and lumped mass configuration design,are proposed and demonstrated.A disk resonator gyroscope prototype is fabricated based on these design strategies,and the sensor itself shows an angle random walk as low as 0.001°/√h,demonstrating true potential to achieve navigation-grade performance.The gyroscope with readout electronics shows an angle random walk of 0.01°/√h and a bias instability of 0.04°/h at room temperature without compensation,revealing that the performance of the gyroscope is severely limited by the readout electronics,which should be further improved.We expect that the quality factor improvement methods can be used in the design of other MEMS gyroscopes and that the newly designed DRG can be further improved to achieve navigation-grade performances for high-end industrial,transportation,aerospace,and automotive applications. 展开更多
关键词 RESONATOR CONSTANT ATTRACTIVE
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Mesoporous tin oxide nanospheres for a NO_x in air sensor 被引量:1
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作者 Haonan Zhang ming zhuo +1 位作者 Yazi Luo Yuejiao Chen 《Journal of Semiconductors》 EI CAS CSCD 2017年第2期20-25,共6页
Mesoporous tin oxide(SnO_2/ with a high surface area of 147.5 m^2/g has been successfully synthesized via self-assembly process, combining the driven forces of water-evaporation and molecular interactions. Scanning e... Mesoporous tin oxide(SnO_2/ with a high surface area of 147.5 m^2/g has been successfully synthesized via self-assembly process, combining the driven forces of water-evaporation and molecular interactions. Scanning electron microscope, X-ray diffraction, transmission electron micrograph, Fourier transform infrared and BrunauerEmmett-Teller were employed to analyze the morphology and crystal structure of the as-synthesized mesoporous materials. As a gas sensor, mesoporous SnO_2 shows impressive performances towards NOx gas with high selectivity and stability as well as ultra high sensitivity about 94.3 to 10 ppm NO_x gas at 300 ℃. The best response time of the sample S-500 is about 3.4 s to 10 ppm NO_x at 450℃. 展开更多
关键词 mesoporous materials tin oxide sensor nanospheres
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