Objective:To study the SDF-1/CXCR4 expression in bladder cancer tissue and the correlation with negative costimulatory molecule PD-L1, cell apoptosis and invasion.Methods: A total of 118 cases of bladder cancer tissue...Objective:To study the SDF-1/CXCR4 expression in bladder cancer tissue and the correlation with negative costimulatory molecule PD-L1, cell apoptosis and invasion.Methods: A total of 118 cases of bladder cancer tissue and para-carcinoma tissue surgically removed in our hospital between May 2014 and May 2016 were selected as the research samples, the RNA was extracted and then reverse-transcribed into cDNA, and the expression levels of SDF-1/CXCR4, PD-L1/PD-1, cell apoptosis-related molecules and cell invasion-related molecules were detected.Results: SDF-1 and CXCR4 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue;PD-L1, PD-1, Rec1, Survivin, MRPS5, Nanog, BCAPP2Ac, TRPM8, TRPV2, ILK,β-catenin and GUGBP1 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue and positively correlated with SDF-1 and CXCR4 mRNA expression.Conclusion:Highly expressed SDF-1/CXCR4 in bladder cancer tissue are closely related to the high expression of negative costimulatory molecule PD-L1, pro-proliferation molecules and pro-invasion molecules, and SDF-1/CXCR4 can promote the immune escape, proliferation and invasion of bladder cancer cells.展开更多
Objective: To study the miR-21 expression in bladder cancer lesion and its effect on cancer cell invasion and angiogenesis. Methods: A total of 94 patients with bladder cancer who received surgical resection in our ho...Objective: To study the miR-21 expression in bladder cancer lesion and its effect on cancer cell invasion and angiogenesis. Methods: A total of 94 patients with bladder cancer who received surgical resection in our hospital between February 2015 and June 2017 were selected as the research subjects, and proper amount of bladder cancer lesion and adjacent lesion were collected to detect the expression of miR-21 as well as the mRNA expression of invasion genes and angiogenesis genes. Results: miR-21 expression in bladder cancer lesion was significantly higher than that in adjacent lesion;CDH13 and CBX7 mRNA expression in bladder cancer lesion were significantly lower than those in adjacent lesion while TRAP-1, KPNA2, TRPM8, EDIL3, HGF, VEGF and Epha2 mRNA expression were significantly higher than those in adjacent lesion. CDH13 and CBX7 mRNA expression in bladder cancer lesion with high miR-21 expression were significantly lower than those in bladder cancer lesion with low miR-21 expression while TRAP-1, KPNA2, TRPM8, EDIL3, HGF, VEGF and Epha2 mRNA expression were significantly higher than those in bladder cancer lesion with low miR-21 expression. Conclusion: The high expression of miR-21 in bladder cancer can promote the invasion of cancer cells and the angiogenesis in the lesion.展开更多
文摘Objective:To study the SDF-1/CXCR4 expression in bladder cancer tissue and the correlation with negative costimulatory molecule PD-L1, cell apoptosis and invasion.Methods: A total of 118 cases of bladder cancer tissue and para-carcinoma tissue surgically removed in our hospital between May 2014 and May 2016 were selected as the research samples, the RNA was extracted and then reverse-transcribed into cDNA, and the expression levels of SDF-1/CXCR4, PD-L1/PD-1, cell apoptosis-related molecules and cell invasion-related molecules were detected.Results: SDF-1 and CXCR4 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue;PD-L1, PD-1, Rec1, Survivin, MRPS5, Nanog, BCAPP2Ac, TRPM8, TRPV2, ILK,β-catenin and GUGBP1 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue and positively correlated with SDF-1 and CXCR4 mRNA expression.Conclusion:Highly expressed SDF-1/CXCR4 in bladder cancer tissue are closely related to the high expression of negative costimulatory molecule PD-L1, pro-proliferation molecules and pro-invasion molecules, and SDF-1/CXCR4 can promote the immune escape, proliferation and invasion of bladder cancer cells.
文摘Objective: To study the miR-21 expression in bladder cancer lesion and its effect on cancer cell invasion and angiogenesis. Methods: A total of 94 patients with bladder cancer who received surgical resection in our hospital between February 2015 and June 2017 were selected as the research subjects, and proper amount of bladder cancer lesion and adjacent lesion were collected to detect the expression of miR-21 as well as the mRNA expression of invasion genes and angiogenesis genes. Results: miR-21 expression in bladder cancer lesion was significantly higher than that in adjacent lesion;CDH13 and CBX7 mRNA expression in bladder cancer lesion were significantly lower than those in adjacent lesion while TRAP-1, KPNA2, TRPM8, EDIL3, HGF, VEGF and Epha2 mRNA expression were significantly higher than those in adjacent lesion. CDH13 and CBX7 mRNA expression in bladder cancer lesion with high miR-21 expression were significantly lower than those in bladder cancer lesion with low miR-21 expression while TRAP-1, KPNA2, TRPM8, EDIL3, HGF, VEGF and Epha2 mRNA expression were significantly higher than those in bladder cancer lesion with low miR-21 expression. Conclusion: The high expression of miR-21 in bladder cancer can promote the invasion of cancer cells and the angiogenesis in the lesion.
