Three-microRNA signature identified by bioinformatics analysis predicts prognosis of gastric cancer patients

AIM To identify multiple micro RNAs(mi RNAs) for predicting the prognosis of gastric cancer(GC) patients by bioinformatics analysis.METHODS The original microarray dataset GSE93415,which included 20 GC and 20 tumor adjacent normal gastric mucosal tissues,was downloaded from the Gene Expression Omnibus database and used for screening differentially expressed mi RNAs(DEMs).The cutoff criteria were P < 0.05 and fold change > 2.0.In addition,we acquired the mi RNA expression profiles and clinical information of 361 GC patients from The Cancer Genome Atlas database to assess the prognostic role of the DEMs.The target genes of mi RNAs were predicted using Target Scan,mi RDB,mi RWalk,and DIANA,and then the common target genes were selected for functional enrichment analysis.RESULTS A total of 110 DEMs including 19 up-regulated and 91 down-regulated mi RNAs were identified between 20 pairs of GC and tumor adjacent normal tissues,and the Kaplan-Meier survival analysis found that a threemi RNA signature(mi R-145-3 p,mi R-125 b-5 p,and mi R-99 a-5 p) had an obvious correlation with the survival of GC patients.Furthermore,univariate and multivariate Cox regression analyses indicated that the three-mi RNA signature could be a significant prognostic marker in GC patients.The common target genes of the three mi RNAs are added up to 108 and used for Gene Functional Enrichment analysis.Biological Process and Molecular Function analyses showed that the target genes are involved in cell recognition,gene silencing and nucleic acid binding,transcription factor activity,and transmembrane receptor activity.Cellular Component analysis revealed that the genes are portion of nucleus,chromatin silencing complex,and TORC1/2 complex.Biological Pathway analysis indicated that the genes participate in several cancer-related pathways,such as the focal adhesion,PI3 K,and m TOR signaling pathways.CONCLUSION This study justified that a three-mi RNA signature could play a role in predicting the survival of GC patients.

Five-Tone Therapy Plus Head and Face Massage Relieves Insomnia of Patients with Heart and Spleen Deficiency

Objective:To explore the therapeutic effect of five-element music therapy based on syndrome differentiation plus head and face massage in relieving insomnia of patients with heart and spleen deficiency.Methods:Sixty patients with heart and spleen deficiency were divided into control group and test group,30 cases each.The control group received routine treatment and nursing.The experimental group was given five-element music therapy plus head and face massage on the basis of the control group,once a day,15 minutes/time,5 times/week,and continuous observation for 4 weeks.The Pittsburgh Sleep Quality Index(PSQI)was used to assess sleep quality before and after intervention.Results:After 30 days,the total effective rate of the experimental group was 86.67%,which was higher than the total effective rate of 76.67%in control group(P<0.05).There were significant differences in the time of sleep,sleep quality,sleep efficiency and PSQI score between the two groups(P<0.05).Conclusion:Five-tone therapy based on syndrome differentiation combined with head and face massage can effectively improve the sleep quality of patients with heart and spleen deficiency,prolong sleep time,with no adverse reactions,so it is worthy of clinical application.

An Imaginary Taiwan From a Composer in China A Case Study of Taiwan Bangzi Opera

Taiwan Bangzi Opera is a branch of Henan Bangzi Opera in Henan, which was transmitted from China to Taiwan within the armies under a political and historical circumstance around 1947. As the trend of localisation in these ten years becomes obvious, several localised performances are created; however, the composers in China do not understand the music culture in Taiwan, so some interesting phenomenon can be found between an imaginary Taiwan from their viewpoints and the real culture in Taiwan. The Sorceress Bride is a performance which adapted so-called the aboriginal tune in 2012, and it arises several issues for further discussion.

Novel long non-coding RNA LINC02532 promotes gastric cancer cell proliferation, migration, and invasion in vitro

BACKGROUND Long non-coding RNAs(lncRNAs) are a kind of single-stranded RNA of more than 200 nucleotides in length and have no protein-coding function. Amounting studies have indicated that lncRNAs could play a vital role in the initiation and development of cancers, including gastric cancer(GC). Considering the crucial functions of lncRNAs, the identification and exploration of novel lncRNAs in GC is necessary.AIM To explore the role of novel lncRNA LINC02532 in GC.METHODS The upregulated LINC02532 was identified by processing the GC RNA-Seq data from The Cancer Genome Atlas. The qRT-PCR assay was performed to confirm the expression levels in GC cell lines and tissues. Cell proliferation, migration,and invasion were evaluated by the cell counting kit-8, colony formation, wound healing, and Transwell assays. The miRNAs downregulated in GC and sponged by LINC02532 were identified from and predicted by the data from the Firehose and RNA22 software programs, respectively. The miRNA downstream target genes were obtained from the TargetScan, miRDB, and DIANA online tools.Gene functional enrichment analysis was carried out using the Database for Annotation, Visualization, and Integrated Discovery software in the categories of cellular components, biological processes, molecular functions, and KEGG pathways.RESULTS The qRT-PCR assay demonstrated that the LINC02532 expression level was significantly upregulated in the GC cell lines and 52 paired tissues. Kaplan-Meiersurvival analysis based on The Cancer Genome Atlas data showed that patients with higher LINC02532 expression had poorer prognosis than those with lower LINC02532 expression. The correlation analysis between expression and clinicopathological features revealed that high expression of LINC02532 was associated with a high TNM stage(P = 0.008) and poor differentiation grade(P =0.023). Functional experiments showed that LINC02532 promoted GC cell proliferation, migration, and invasion. According to the bioinformatics analysis,LINC02532 may act as a ceRNA by sponging downregulated miR-129-5 p and miR-490-5 p. Target genes of the two miRNAs were selected for further functional enrichment analysis. Importantly, KEGG pathway analysis showed that the genes were mainly involved in transcriptional misregulation in cancer, cell cycle, and TGF-beta, mTOR, and p53 signaling pathways.CONCLUSION The present study suggested that LINC02532 acted as an oncogene in GC and may be a promising target for therapy and prognosis management of GC.

Identification and prediction of novel non-coding and coding RNA-associated competing endogenous RNA networks in colorectal cancer

AIM To identify and predict the competing endogenous RNA(ce RNA) networks in colorectal cancer(CRC) by bioinformatics analysis. METHODS In the present study, we obtained CRC tissue and normal tissue gene expression profiles from The Cancer Genome Atlas project. Differentially expressed(DE) genes(DEGs) were identified. Then, upregulated and downregulated mi RNA-centered ceRNA networks were constructed by analyzing the DEGs using multiple bioinformatics approaches. DEm RNAs in the ceRNA networks were identified in Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways using KEGG Orthology Based Annotation System 3.0. The interactions between proteins were analyzed using the STRING database. Kaplan-Meier survival analysis was conducted for DEGs and real time quantitative polymerase chain reaction(RT-qPCR) was also performed to validate the prognosis-associated lncRNAs in CRC cell lines.RESULTS Eighty-one DElncRNAs, 20 DEmiRNAs, and 54 DEmRNAs were identified to construct the ceRNA networks of CRC. The KEGG pathway analysis indicated that nine out of top ten pathways were related with cancer and the most significant pathway was "colorectal cancer". Kaplan-Meier survival analysis showed that the overall survival was positively associated with five DEGs(IGF2-AS, POU6 F2-AS2, hsa-miR-32, hsa-miR-141, and SERPINE1) and it was negatively related to three DEGs(LINC00488, hsamiR-375, and PHLPP2). Based on the STRING protein database, it was found that SERPINE1 and PHLPP2 interact with AKT1. Besides, SERPINE1 can interact with VEGFA, VTN, TGFB1, PLAU, PLAUR, PLG, and PLAT. PHLPP2 can interact with AKT2 and AKT3. RT-qPCR revealed that the expression of IGF2-AS, POU6 F2-AS2, and LINC00488 in CRC cell lines was consistent with the in silico results.CONCLUSION Ce RNA networks play an important role in CRC. Multiple DEGs are related with clinical prognosis, suggesting that they may be potential targets in tumor diagnosis and treatment.

Correction to“Novel long non-coding RNA LINC02532 promotes gastric cancer cell proliferation,migration,and invasion in vitro”

We have replaced the misapplied images and the revised Figure 3 is provided.