Interaction of hispidin with pepsin:Multi-spectroscopic analyses and docking simulation

Hispidin is a pyranone compound found in edible and medicinal mushrooms of the Phellinus and Inonotus genera.This investigation used fluorescence spectroscopy,UV absorption spectroscopy,and molecular docking to examine the interaction of hispidin with pepsin.The Stern-Volmer method was used to perform the fluorescence quenching measurements at different temperatures(298 K,303 K,and 310 K).According to the findings,hispidin induced a static quenching mechanism in pepsin that resulted in the creation of a hispidin-pepsin complex with binding constants(Ka)ranging from 9.56×10^(4) to 3.45×10^(5) L mol^(-1).The positive values ofΔH(84.6 kJ mol-1)andΔS(337.9 J mol^(-1) K^(-1))demonstrated that hydrophobic forces contributed to forming the hispidin-pepsin complex.The findings of UV-vis absorption,synchronous fluorescence,and 3D fluorescence spectraspectra demonstrated that hispidin altered the conformation and microenvironment of pepsin.According to the analysis of molecular docking,hispidin got into the pepsin's active cavity.The research clarifies the molecular mechanisms by which hispidin binds to pepsin and helps understand its possible biological activity in vivo.

非肺静脉触发灶消融联合肺静脉隔离对低射血分数心房颤动患者的影响

目的探究肺静脉隔离联合非肺静脉触发灶消融对低射血分数的阵发性心房颤动(以下简称房颤)患者远期预后的影响。方法选取天津市第五中心医院接受导管消融术且左室射血分数(LVEF)<45%的阵发性房颤患者87例,根据治疗方式的不同分为Ⅰ组(肺静脉隔离联合非肺静脉触发消融)及Ⅱ组(肺静脉隔离)。随访2年,运用Log-rankχ~2比较两组未复发率的差异,Cox回归识别低射血分数的阵发性房颤患者术后复发的影响因子。结果两组患者随访后2年手术成功率、非肺静脉触灶及LVEF比较,差异有统计学意义(P<0.05)。Ⅰ组未复发率高于Ⅱ组(P<0.05)。冠状动脉疾病、非肺静脉触发灶、非肺静脉触发灶消融及LVEF是低射血分数合并阵发性房颤患者术后复发的独立影响因素(P<0.05)。结论肺静脉隔离联合非肺静脉触发灶消融对低射血分数的房颤患者远期疗效较好,值得临床推广。

Analysis of spudcan-footprint interaction in a single soil with nonlinear FEM

The footprints that remain on the seabed after offshore jack-up platforms completed operations and moved out provide a significant risk for any futurej ack-up installation at that site. Detrimental horizontal and/or rotational loads will be induced on the base cone of the jack-up platform leg （spudcan） in the preloading process where only vertical loads are normally expected. However, there are no specific guidelines on design of spudcan re-installation very close to or partially overlapping existing footprints. This paper presents a rational design approach for assessing spudcan-footprint interaction and the failure process of foundation in a single layer based on nonlinear finite element method. The rela- tionship between the distance between the spudcan and the footprint and the horizontal sliding force has been obtained. Comparisons of simulation and experimental results show that the model in this paper can deal well with the combined problems of sliding friction contact, fluid-solid coupling, and convergence difficulty. The analytical results may be useful to jack-up installation workovers close to existing footprints.

Observational results of MUSER during 2014–2019

The solar radio signal that can be received by the ground-based telescopes covers a wide frequency range,allowing us to monitor the complex physical processes occurred from the solar surface to the vast interplanetary space.MingantU SpEctral Radioheliograph(MUSER),as the latest generation of solar dedicated radio spectral-imaging instrument in the centimeter-decimeter wavelengths,has accumulated a large number of observational data since its commissioning observation in 2014.This paper presents the main observational results identified by MUSER from 2014 to 2019,including the quiet Sun and 94 solar radio burst events.We find that there are 81 events accompanied with Geostationary Operational Environmental Satellites(GOES)soft X-ray(SXR)flares,among which the smallest flare class is B1.0.There are 13 events without accompanying any recorded flares,among which the smallest SXR intensity during the radio burst period is equivalent to level-A.The main characteristics of all radio burst events are presented,which shows the powerful ability of MUSER to capture the valuable information of the solar non-thermal processes and the importance for space weather.This work also provides a database for further in-depth research.

The Mechanisms of Pei-yuan-Tong-nao Capsule as a Therapeutic Agent against Cerebrovascular Disease

Objective:Pei-Yuan-Tong-Nao(PYTN)capsule has been widely used for the treatment of cerebrovascular disease(CVD),including cerebral ischemia.However,due to the complexity of traditional Chinese Medicine(TCM)and the lack of proper inspection methods,the pharmacological mechanisms of the actions of PYTN capsule on CVD remain ambiguous.In this investigation,a network pharmacology method was used to investigate the mechanism of action of PYTN capsule in the treatment of CVD.Methods:In this method,a chemical similarity ensemble approach was employed to predict putative targets for chemicals derived from PYTN capsule.Results:Thus,a total of 351 compounds and 650 proteins were identified as potential active ingredients and putative CVD-related targets.In addition,two interaction networks were constructed,including a network between putative targets of PYTN capsule and known CVD-related targets,and a network between candidate active compounds and putative CVD-related targets.Conclusion:The mechanism of PYTN capsule in the treatment of CVD was found to be based on three functional modules,namely,antioxidation,anti-inflammation,and antiapoptosis modules through multiple signaling pathways,such as PI3 K-Akt,mitogen-activated protein kinase,and TNF signaling pathways.We hope that this work can provide insight into the pharmacological mechanisms of TCMs in the treatment of CVD and provide a basis for further development and the discovery of more effective clinical strategies for the treatment of complicated diseases.