AIM: To explore the biological behavior of gastric carcinoma micrometastasis (MM) with a marker of cytokeratin 18 (CK18) and to evaluate the clinical stage of gastric carcinoma and its prognosis. METHODS: Reverse tran...AIM: To explore the biological behavior of gastric carcinoma micrometastasis (MM) with a marker of cytokeratin 18 (CK18) and to evaluate the clinical stage of gastric carcinoma and its prognosis. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the expression of CK18 mRNA in 298 lymph nodes from 35 patients with gastric carcinoma and 20 lymph nodes from 10 patients with chronic peptic ulcer and gastric perforation diagnosed by pathological examination and surgery.CK18 mRNA expression of peripheral blood from 54 patients with gastric carcinoma and 10 healthy people were also examined.RESULTS: Expression of CK18 mRNA was not found in 10 patients with benign pathological changes.CK18 mRNA expression in gastric carcinoma tissues was strongly positive. In gastric carcinoma patients,pathological examination revealed that 99 of 298 (33.2%)lymph nodes were positive, while RT-PCR showed that 133 of 298 (44.6%) lymph nodes had expression of CK18 mRNA. The difference was significant (P＜0.05).Among the 199 negative lymph nodes identified by pathological examinations, 34 (17.1%) displayed positive expression of CK18 mRNA by RT-PCR. The positive expression of CK18 mRNA was associated with lymph node micrometastasis (LMM) of gastric carcinoma. CK18 mRNA was negatively expressed in all 10 healthycases and positively expressed in 38.9% of 54 blood specimens from gastric carcinoma patients. The positive rate was not correlated with tumor invasion of gastric carcinoma,but was significantly associated with TNM stage, lymph node metastasis (P=0.0290, P＜ 0.05) and tumor differentiation (P= 0.2956, P＜0.05).CONCLUSION: RT-PCR with CK18 mRNA as a molecular marker is highly sensitive and specific in detecting LMM of gastric carcinoma. It can benefit the diagnosis of MM and guide studies on biological behavior, clinical phase,and therapy as well as relapse monitoring.展开更多
We report the multiplicity dependence of charged particle production for the n~±, K~±, p, , and ? mesons at |y|<1:0 in p + p collisions at s^(1/2) = 200 GeV from a PYTHIA simulation. The impact of multipl...We report the multiplicity dependence of charged particle production for the n~±, K~±, p, , and ? mesons at |y|<1:0 in p + p collisions at s^(1/2) = 200 GeV from a PYTHIA simulation. The impact of multiple parton interactions and gluon contributions is studied and found to be a possible source of the splitting of the particle yields as a function of p_T with respect to the multiplicity. No obvious particle species dependence of the splitting is observed.The multiplicity dependence of the ratios Kˉ/πˉ, K^+/π^+,/πˉ, p/π^+, and K_s^0 at mid-rapidity in p+ p collisions is found to follow a tendency similar to that in Au t Au collisions at (s_(NN))^(1/2) = 200 GeV at the Relativistic Heavy Ion Collider, indicating similar underlying initial production mechanisms despite the differences in the initial colliding systems.展开更多
Reducing thermal conductivity while avoiding adverse interfacial reactions during sintering is crucial for improving the thermoelectric performance of Bi_(2)Te_(3)based composites.Inert ceramic nanoparticles are good ...Reducing thermal conductivity while avoiding adverse interfacial reactions during sintering is crucial for improving the thermoelectric performance of Bi_(2)Te_(3)based composites.Inert ceramic nanoparticles are good candidates for achieving this goal.In this study,we designed and prepared a series of p-type Bi_(0.5)Sb_(1.5)Te_(3)nanocomposites decorated with Y_(2)O_(3)ceramic nanoparticles via ball-milling dispersion and spark-plasma sintering.Owing to the chemical stability of the ceramics,no traces of atomic doping or interfacial reactions were observed.Transport measurements revealed that the Y_(2)O_(3)nanoparticles distributed along the grain boundaries acted as energy-dependent carrier-filtering centers to improve the scattering parameter and Seebeck coefficient,contributing to the elevated power factor even with a decreased electrical conductivity.Moreover,the incorporated Y_(2)O_(3)nanoparticles and various defect structures they induced effectively strengthened the phonon scattering and suppressed the lattice thermal conductivity.Consequently,a peak figure of merit(ZT)of 1.23 at 313 K was achieved for 0.4%Y_(2)O_(3)/Bi_(0.5)Sb_(1.5)Te_(3),which is 13%higher than that of the matrix.In addition,the Vickers hardness of the composite material was 35%higher than that of the matrix.This study demonstrates the effectiveness of ceramic nanoparticles in synergistic ally improving the thermoelectric and mechanical properties,which may be further extended to other thermoelectric systems.展开更多
基金Supported by the Natural Science Foundation of Jilin Province,No. 20010594
文摘AIM: To explore the biological behavior of gastric carcinoma micrometastasis (MM) with a marker of cytokeratin 18 (CK18) and to evaluate the clinical stage of gastric carcinoma and its prognosis. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the expression of CK18 mRNA in 298 lymph nodes from 35 patients with gastric carcinoma and 20 lymph nodes from 10 patients with chronic peptic ulcer and gastric perforation diagnosed by pathological examination and surgery.CK18 mRNA expression of peripheral blood from 54 patients with gastric carcinoma and 10 healthy people were also examined.RESULTS: Expression of CK18 mRNA was not found in 10 patients with benign pathological changes.CK18 mRNA expression in gastric carcinoma tissues was strongly positive. In gastric carcinoma patients,pathological examination revealed that 99 of 298 (33.2%)lymph nodes were positive, while RT-PCR showed that 133 of 298 (44.6%) lymph nodes had expression of CK18 mRNA. The difference was significant (P＜0.05).Among the 199 negative lymph nodes identified by pathological examinations, 34 (17.1%) displayed positive expression of CK18 mRNA by RT-PCR. The positive expression of CK18 mRNA was associated with lymph node micrometastasis (LMM) of gastric carcinoma. CK18 mRNA was negatively expressed in all 10 healthycases and positively expressed in 38.9% of 54 blood specimens from gastric carcinoma patients. The positive rate was not correlated with tumor invasion of gastric carcinoma,but was significantly associated with TNM stage, lymph node metastasis (P=0.0290, P＜ 0.05) and tumor differentiation (P= 0.2956, P＜0.05).CONCLUSION: RT-PCR with CK18 mRNA as a molecular marker is highly sensitive and specific in detecting LMM of gastric carcinoma. It can benefit the diagnosis of MM and guide studies on biological behavior, clinical phase,and therapy as well as relapse monitoring.
基金supported by the Major State Basic Research Development Program in China(No.2014CB845400)the National Natural Science Foundation of China(No.11375184)+2 种基金the Youth Innovation Promotion Association Fund of CAS(No.CX2030040079)the Ministry of Science and Technology(Mo ST)of China(No.2016YFE0104800)the Science and Technological Fund of Anhui Province for Outstanding Youth(No.1808085J02)
文摘We report the multiplicity dependence of charged particle production for the n~±, K~±, p, , and ? mesons at |y|<1:0 in p + p collisions at s^(1/2) = 200 GeV from a PYTHIA simulation. The impact of multiple parton interactions and gluon contributions is studied and found to be a possible source of the splitting of the particle yields as a function of p_T with respect to the multiplicity. No obvious particle species dependence of the splitting is observed.The multiplicity dependence of the ratios Kˉ/πˉ, K^+/π^+,/πˉ, p/π^+, and K_s^0 at mid-rapidity in p+ p collisions is found to follow a tendency similar to that in Au t Au collisions at (s_(NN))^(1/2) = 200 GeV at the Relativistic Heavy Ion Collider, indicating similar underlying initial production mechanisms despite the differences in the initial colliding systems.
基金financially supported by the National Natural Science Foundation of China(Nos.11834012 and 52130203)。
文摘Reducing thermal conductivity while avoiding adverse interfacial reactions during sintering is crucial for improving the thermoelectric performance of Bi_(2)Te_(3)based composites.Inert ceramic nanoparticles are good candidates for achieving this goal.In this study,we designed and prepared a series of p-type Bi_(0.5)Sb_(1.5)Te_(3)nanocomposites decorated with Y_(2)O_(3)ceramic nanoparticles via ball-milling dispersion and spark-plasma sintering.Owing to the chemical stability of the ceramics,no traces of atomic doping or interfacial reactions were observed.Transport measurements revealed that the Y_(2)O_(3)nanoparticles distributed along the grain boundaries acted as energy-dependent carrier-filtering centers to improve the scattering parameter and Seebeck coefficient,contributing to the elevated power factor even with a decreased electrical conductivity.Moreover,the incorporated Y_(2)O_(3)nanoparticles and various defect structures they induced effectively strengthened the phonon scattering and suppressed the lattice thermal conductivity.Consequently,a peak figure of merit(ZT)of 1.23 at 313 K was achieved for 0.4%Y_(2)O_(3)/Bi_(0.5)Sb_(1.5)Te_(3),which is 13%higher than that of the matrix.In addition,the Vickers hardness of the composite material was 35%higher than that of the matrix.This study demonstrates the effectiveness of ceramic nanoparticles in synergistic ally improving the thermoelectric and mechanical properties,which may be further extended to other thermoelectric systems.
