AIM: To investigate the expressions of micro RNA-20a(mi R-20a) and let-7a in esophageal squamous cell carcinoma(ESCC) and their diagnostic value. METHODS: Seventy patients with ESCC and 40 healthy subjects were enroll...AIM: To investigate the expressions of micro RNA-20a(mi R-20a) and let-7a in esophageal squamous cell carcinoma(ESCC) and their diagnostic value. METHODS: Seventy patients with ESCC and 40 healthy subjects were enrolled to investigate the expression of mi R-20 a and let-7a using quantitative real-time PCR. The expression of mi R-20 a and let-7a was compared between ESCC patients and healthy subjects. The plasma levels of mi R-20 a and let-7a in relation to patient clinicopathologic parameters, the receiver operating characteristic(ROC) curve, and the sensitivity and specificity of mi R-20 a and let-7a in ESCC diagnosis were analyzed.RESULTS: Plasma levels of mi R-20 a were significantly higher in ESCC patients than in healthy controls, and plasma levels of let-7 were lower in ESCC patients than in healthy controls(both P < 0.05). The area under the ROC curve of mi R-20 a was 0.767(95%CI: 0.677-0.857; P < 0.001), when the cut-off value was set at 4.77, the sensitivity and specificity were 64.3% and 75.0%, respectively. The area under the ROC curve of let-7a was 0.829(95%CI: 0.754-0.904; P < 0.001), when the cut-off value was set at 6.22, the sensitivity and specificity were 74.3% and 85.0%, respectively. Thus, the sensitivity and specificity of let-7a were higher than those of mi R-20 a. The median relative plasma expression of let-7a in clinical stage Ⅲ/Ⅳ(0.24) was lower than that in stage Ⅰ/Ⅱ(0.42), while the expression of mi R-20 a according to stage was not statistically different. The expressions of mi R-20 a and let-7a were not related to gender, age, tumor diameter, tumor grade, or pathologic stage.CONCLUSION: Plasma mi R-20 a and let-7a levels are significantly altered in patients with ESCC and can be used as potential biomarkers in the diagnosis of ESCC.展开更多
基金Supported by Medical Scientific Research Foundation of Health Department of Henan Province of China,No.201403077
文摘AIM: To investigate the expressions of micro RNA-20a(mi R-20a) and let-7a in esophageal squamous cell carcinoma(ESCC) and their diagnostic value. METHODS: Seventy patients with ESCC and 40 healthy subjects were enrolled to investigate the expression of mi R-20 a and let-7a using quantitative real-time PCR. The expression of mi R-20 a and let-7a was compared between ESCC patients and healthy subjects. The plasma levels of mi R-20 a and let-7a in relation to patient clinicopathologic parameters, the receiver operating characteristic(ROC) curve, and the sensitivity and specificity of mi R-20 a and let-7a in ESCC diagnosis were analyzed.RESULTS: Plasma levels of mi R-20 a were significantly higher in ESCC patients than in healthy controls, and plasma levels of let-7 were lower in ESCC patients than in healthy controls(both P < 0.05). The area under the ROC curve of mi R-20 a was 0.767(95%CI: 0.677-0.857; P < 0.001), when the cut-off value was set at 4.77, the sensitivity and specificity were 64.3% and 75.0%, respectively. The area under the ROC curve of let-7a was 0.829(95%CI: 0.754-0.904; P < 0.001), when the cut-off value was set at 6.22, the sensitivity and specificity were 74.3% and 85.0%, respectively. Thus, the sensitivity and specificity of let-7a were higher than those of mi R-20 a. The median relative plasma expression of let-7a in clinical stage Ⅲ/Ⅳ(0.24) was lower than that in stage Ⅰ/Ⅱ(0.42), while the expression of mi R-20 a according to stage was not statistically different. The expressions of mi R-20 a and let-7a were not related to gender, age, tumor diameter, tumor grade, or pathologic stage.CONCLUSION: Plasma mi R-20 a and let-7a levels are significantly altered in patients with ESCC and can be used as potential biomarkers in the diagnosis of ESCC.
