The Asian swamp eel(Monopterus albus) is one of the most economically important freshwater fish in East Asia,but data on the immune genes of M.albus are scarce compared to other commercially important fish.A better un...The Asian swamp eel(Monopterus albus) is one of the most economically important freshwater fish in East Asia,but data on the immune genes of M.albus are scarce compared to other commercially important fish.A better understanding of the eel's immune responses may help in developing strategies for disease management,potentially improving yields and mitigating losses.In mammals,interferon regulatory factors(IRFs) play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRF10 have seldom been studied.In this study,we characterized IRF4 and IRF10 from M.albus(maIRF4 and maIRF10) and found that maIRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid(aa) protein,while maIRF10 consists of 1 744 nucleotides including an open reading frame(ORF) of 1 236 nt encoding 411 aa.The maIRF10 gene was constitutively expressed at high levels in a variety of tissues,while maIRF4 showed a very limited expression pattern.Expression of maIRF4 and maIRF10 in head kidney,and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic: polycytidylic acid(poly I:C),lipopolysaccharide(LPS) and a common pathogenic bacteria Aeromonas hydrophila.These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M.albus.展开更多
Dear Editor,Quorum sensing(QS)is a widespread phenomenon in bacteria which enables them to participate in cell-to-cell communication by producing and responding to small signal molecules,thus synchronously altering th...Dear Editor,Quorum sensing(QS)is a widespread phenomenon in bacteria which enables them to participate in cell-to-cell communication by producing and responding to small signal molecules,thus synchronously altering their behavior depending on population density(Singh et al.,2000;Miller and Bassler,2001).Through QS,bacteria coordinate processes such as expression of virulenee factors(Slamti and Lereclus,2002),biofilm formation(Parashar et al.,2011),sporulation(Perego et al.,1996),conjugation(Kozlowicz et al.,2006),antibiotic synthesis(Miller and Bassler,2001;Whiteley et al.,2017)etc.展开更多
基金financially supported by the Project from the National Natural Science Foundation of China(31101928)the State Key Laboratory of Freshwater Ecology and Biotechnology(2010FB02)Public Welfare Scientific Research Project of Hubei Province(2012DBA29001)
文摘The Asian swamp eel(Monopterus albus) is one of the most economically important freshwater fish in East Asia,but data on the immune genes of M.albus are scarce compared to other commercially important fish.A better understanding of the eel's immune responses may help in developing strategies for disease management,potentially improving yields and mitigating losses.In mammals,interferon regulatory factors(IRFs) play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRF10 have seldom been studied.In this study,we characterized IRF4 and IRF10 from M.albus(maIRF4 and maIRF10) and found that maIRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid(aa) protein,while maIRF10 consists of 1 744 nucleotides including an open reading frame(ORF) of 1 236 nt encoding 411 aa.The maIRF10 gene was constitutively expressed at high levels in a variety of tissues,while maIRF4 showed a very limited expression pattern.Expression of maIRF4 and maIRF10 in head kidney,and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic: polycytidylic acid(poly I:C),lipopolysaccharide(LPS) and a common pathogenic bacteria Aeromonas hydrophila.These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M.albus.
基金the Ministry of Science and Technology of China grants 2014CB910400 and the National Nature Science Foundation of China grants 31770948,31570875,31800159 and 81590761.
文摘Dear Editor,Quorum sensing(QS)is a widespread phenomenon in bacteria which enables them to participate in cell-to-cell communication by producing and responding to small signal molecules,thus synchronously altering their behavior depending on population density(Singh et al.,2000;Miller and Bassler,2001).Through QS,bacteria coordinate processes such as expression of virulenee factors(Slamti and Lereclus,2002),biofilm formation(Parashar et al.,2011),sporulation(Perego et al.,1996),conjugation(Kozlowicz et al.,2006),antibiotic synthesis(Miller and Bassler,2001;Whiteley et al.,2017)etc.
