Effects of TRPC6 on invasibility of low-differentiated prostate cancer cells

Objective:To study the expression of TRPC6 among prostate cancer cells,establish high expression cell lines of TRPC6,and to provide potential cell mode for prostate cancer oncogenesis and development.Methods:Occurrence and development of prostate cancer cells,PC3,PC—3m DU145,22 rvl,LNCaP and normal prostate epithelial cells in the PrEC TRPC6 expression level were detected by QPCR method.Calcium phosphate transfection method was used to package retrovirus pLEGFP-Nl-TRPC6 and pLEGFP-Nl-vector and infect the prostate cancer cells,a stable high expression of TRPC6 prostate cancer cells.Sable cell lines of TRPC6,matrix metalloproteinase(MMP)2,MMP9 expression was detected by QPCR and Western blot.Change of cell invasion ability was detected by Transwell.Results:The expression level of prostate cancer cells TRPC6 were higher than control group PrEC cells.Among TPRC6 the expression of cell line PC 3 transfer potential wre the lowest,and high transfer cell tone PC-3M express was the highest.Real-time fluorescent quantitative PCR and western blot results showed that after filter,the seventh generation of cell TRPC6 protein and mRNA expression levels were higher than the control group obviously.Transwell experimental results showed that the overexpression of TRPC6could promote the invasion ability of PC.3 prostate cancer cells.Conclusions:TRPC6 expressed in prostate cancer cells is in disorder,and its action may be associated with the invasion and metastasis of prostate cancer cells;successful establishment of stable high expression of TRPC6prostate cancer cells primarily confirm the invasion-trigger ability of TRPC6 on prostate cancer,and lay down the foundation for exploring the TRPC6's role in the occurrence and development of prostate cancer

ZNF267在膀胱癌中的表达及其对细胞增殖和转移的影响

目的研究锌指蛋白267(ZNF267)在膀胱移行细胞癌(TCC)组织及细胞中的表达,及其对TCC细胞增殖、凋亡、周期、转移及侵袭的影响。方法通过实时荧光定量聚合酶链反应检测TCC组织及细胞中ZNF267的表达,并利用si RNA沉默TCC中ZNF267的表达。采用CCK-8法检测ZNF267对TCC细胞增殖的影响,流式细胞术检测ZNF267对TCC细胞凋亡及周期的影响,Transwell实验检测ZNF267对TCC细胞转移和侵袭的影响。结果 TCC组织和细胞中ZNF267的表达水平高于癌旁组织和膀胱上皮永生化细胞系。沉默TCC细胞中ZNF267的表达后,细胞的增殖能力受抑制,而凋亡能力增强。此外,与ZNF267-NC组细胞相比,转染ZNF267 si RNA的TCC细胞周期被阻滞于G1期,细胞转移和侵袭能力受抑制。结论 ZNF267在TCC中表达上调,并可促进TCC细胞的增殖和转移。