Background:The treatment alternatives for bladder cancer(BLCA),the 10th most prevalent cancer in the world,need to be further investigated,and many active substances like Puerarin in herbal medicine were found to be e...Background:The treatment alternatives for bladder cancer(BLCA),the 10th most prevalent cancer in the world,need to be further investigated,and many active substances like Puerarin in herbal medicine were found to be effective in treating BLCA.The purpose of this study was to investigate the potential treating mechanisms of Puerarin on BLCA.Methods:The cell counting kit 8 assay and flow cytometry were performed to confirm Puerarin’s ability to suppress BLCA.The differentially expressed proteins(DEPs)were obtained by Tandem Mass Tags technology and functional enrichment analysis performed by R studio.The most enriched pathways were selected for study and the DEPs were screened out.Protein-protein interaction network maps were created using String and Cytoscape and key proteins,which will be analyzed for survival,expression,and upstream transcription factor prediction,were screened out using the cytoHubba plugin.CHEA3 was used to obtain upstream transcription factor validated by molecular docking and western blotting experiments.Results:Cell counting kit 8 showed that Puerarin inhibited BLCA cells,with 50%inhibitory concentration of 218μmol/L in T24 and 198μmol/L in 5637.Flow cytometry reveals that Puerarin blocks T24 and 5637 cells in G1 phase.1,385 DEPs were obtained and the enrichment analysis revealed that cell cycle and DNA replication were the two main areas in which DEPs were enriched.Cyclin-B-cyclin dependent kinase 1(CDK1),cyclin B1(CCNB1),and polo-like kinase 1(PLK1)were identified as key proteins,and their upstream transcription factor was predicted to be centromere protein A(CENPA).Puerarin’s binding energy to CENPA was determined by molecular docking to be−6.3 kcal/mol,indicating a strong binding interaction.Western blot showed that Puerarin significantly reduced the expression of CENPA.Conclusion:We hypothesize that Puerarin may inhibit the proliferation of bladder cancer cells by inhibiting CENPA expression to regulate PLK1 and CCNB1 expression,thereby affecting cell cycle.展开更多
BACKGROUND Myotonic dystrophy type 1(DM1)is a genetic neuromuscular disease involving multiple systems,especially the cardiopulmonary system.The clinical phenotype of DM1 patients is highly variable,which limits early...BACKGROUND Myotonic dystrophy type 1(DM1)is a genetic neuromuscular disease involving multiple systems,especially the cardiopulmonary system.The clinical phenotype of DM1 patients is highly variable,which limits early diagnosis and treatment.In the present study,we reported a 35-year-old female DM1 patient with dyspnea as the primary onset clinical manifestation,analyzed her family's medical history,and reviewed related literature.CASE SUMMARY A 35-year-old woman was admitted to the hospital with dyspnea of 1 mo duration,and sleep apnea for 3 d.Her respiratory pattern and effort were normal,but limb muscle tension was low.Investigation into the patient's medical history revealed that she might have hereditary neuromuscular disease.Electromyography showed that her myotonia potentials were visible in the resting state of the examined muscles,with decreased motor unit potential time limit and amplitude.Genetic testing for DM1 revealed that the cytosine-thymine-guanine(CTG)repeat number of the DMPK gene exceeded 50,while cytosine-CTG expansion in intron 1 of ZNF9 gene was<30 repeats.The patient was diagnosed with DM1.CONCLUSION DM1 is a genetic neuromuscular disease involving multiple systems,and the clinical phenotype in DM1 is extremely variable.Some patients with DM1 may be presented at the respiratory department because of dyspnea,which should be cautioned by the pulmonologists.There may be no obvious or specific symptoms in the early stage of disease,and clinicians should improve their understanding of DM1 and make an early diagnosis,which will improve patients’quality of life.展开更多
The objective of this paper is to introduce elementary discrete reflected backward equations and to give a simple method to discretize in time a （continuous） reflected backward equation. A presentation of numerical ...The objective of this paper is to introduce elementary discrete reflected backward equations and to give a simple method to discretize in time a （continuous） reflected backward equation. A presentation of numerical simulations is also described.展开更多
Background:The Shanghai growth standards are higher than World Health Organization (WHO) growth standards,which may influence the feeding practices of the caregivers and increase the risk of overweight in these infant...Background:The Shanghai growth standards are higher than World Health Organization (WHO) growth standards,which may influence the feeding practices of the caregivers and increase the risk of overweight in these infants.This study aimed to compare the effects of different growth standards on childhood obesity in Shanghai metropolitan area. Methods:This was a cluster-randomized controlled trial conducted in 2 downtown areas with 19 community health service centers in Shanghai from November 2013 to December 2015.Randomization was done at the level of the community.Infants (health newborns) were assessed and monitored by the Shanghai growth standards (S-group) and the 2006 WHO growth standards (W-group),respectively.Measurements were taken at 1.0,2.0,4.0,6.0,9.0 and 12.0 months of age during follow-up period.Based on the values of length and weight measurements,according to the group’s growth standards,doctors provided the caregivers with corresponding clinical consultation.Changes in weight-for-age z-score (WAZ),length-for-age z-score (LAZ),and weight-for length z-score (WLZ) between 2 groups were assessed using mixed regression models.Overweight was compared between 2 group at all follow-up measurements. Results:A total of 6509 infants (52.1% were boys) were in the W-group,and 8510 infants (51.4% were boys) were in the S-group.The overweight ratios between two groups were distinct at 9 months of age (3.4% in W-group and 4.3% in S-group) and 12 months of age (2.2% in W-group and 3.8% in S-group),and the differences were statistically significant (P= 0.020 and P<0.001,respectively).Compared to W-group,the increase in WAZ (coefficient= 0.04,P=0.004) and WLZ (coefficient= 0.09,P <0.001) were significantly greater,and the LAZ was lower (coefficient = -0.04,P = 0.047) in S-group (W-group values were used as reference in mixed regression models). Conclusion:Compared to the Shanghai growth standards,the adoption of WHO 2006 growth standards would reduce the risk of infant overweight in Shanghai metropolitan area up to 1 year of age.展开更多
基金supported by National Natural Science Fund Item Number(82004110)Xuzhou Science and Technology Plan Project(KC22096)+3 种基金China Postdoctoral Science Foundation(2022M722674)Xuzhou Medical Reserve Talents Project(XWRCHT20220009)the Xuzhou Clinical Medicine Expert Team Project(2018TD004)Peixian Science and Technology Program(P202410)。
文摘Background:The treatment alternatives for bladder cancer(BLCA),the 10th most prevalent cancer in the world,need to be further investigated,and many active substances like Puerarin in herbal medicine were found to be effective in treating BLCA.The purpose of this study was to investigate the potential treating mechanisms of Puerarin on BLCA.Methods:The cell counting kit 8 assay and flow cytometry were performed to confirm Puerarin’s ability to suppress BLCA.The differentially expressed proteins(DEPs)were obtained by Tandem Mass Tags technology and functional enrichment analysis performed by R studio.The most enriched pathways were selected for study and the DEPs were screened out.Protein-protein interaction network maps were created using String and Cytoscape and key proteins,which will be analyzed for survival,expression,and upstream transcription factor prediction,were screened out using the cytoHubba plugin.CHEA3 was used to obtain upstream transcription factor validated by molecular docking and western blotting experiments.Results:Cell counting kit 8 showed that Puerarin inhibited BLCA cells,with 50%inhibitory concentration of 218μmol/L in T24 and 198μmol/L in 5637.Flow cytometry reveals that Puerarin blocks T24 and 5637 cells in G1 phase.1,385 DEPs were obtained and the enrichment analysis revealed that cell cycle and DNA replication were the two main areas in which DEPs were enriched.Cyclin-B-cyclin dependent kinase 1(CDK1),cyclin B1(CCNB1),and polo-like kinase 1(PLK1)were identified as key proteins,and their upstream transcription factor was predicted to be centromere protein A(CENPA).Puerarin’s binding energy to CENPA was determined by molecular docking to be−6.3 kcal/mol,indicating a strong binding interaction.Western blot showed that Puerarin significantly reduced the expression of CENPA.Conclusion:We hypothesize that Puerarin may inhibit the proliferation of bladder cancer cells by inhibiting CENPA expression to regulate PLK1 and CCNB1 expression,thereby affecting cell cycle.
文摘BACKGROUND Myotonic dystrophy type 1(DM1)is a genetic neuromuscular disease involving multiple systems,especially the cardiopulmonary system.The clinical phenotype of DM1 patients is highly variable,which limits early diagnosis and treatment.In the present study,we reported a 35-year-old female DM1 patient with dyspnea as the primary onset clinical manifestation,analyzed her family's medical history,and reviewed related literature.CASE SUMMARY A 35-year-old woman was admitted to the hospital with dyspnea of 1 mo duration,and sleep apnea for 3 d.Her respiratory pattern and effort were normal,but limb muscle tension was low.Investigation into the patient's medical history revealed that she might have hereditary neuromuscular disease.Electromyography showed that her myotonia potentials were visible in the resting state of the examined muscles,with decreased motor unit potential time limit and amplitude.Genetic testing for DM1 revealed that the cytosine-thymine-guanine(CTG)repeat number of the DMPK gene exceeded 50,while cytosine-CTG expansion in intron 1 of ZNF9 gene was<30 repeats.The patient was diagnosed with DM1.CONCLUSION DM1 is a genetic neuromuscular disease involving multiple systems,and the clinical phenotype in DM1 is extremely variable.Some patients with DM1 may be presented at the respiratory department because of dyspnea,which should be cautioned by the pulmonologists.There may be no obvious or specific symptoms in the early stage of disease,and clinicians should improve their understanding of DM1 and make an early diagnosis,which will improve patients’quality of life.
基金Supported by the National Basic Research Programme (No.2007CB814902).
文摘The objective of this paper is to introduce elementary discrete reflected backward equations and to give a simple method to discretize in time a （continuous） reflected backward equation. A presentation of numerical simulations is also described.
基金a grant from the National Natural Science Foundation of China (Nos.81172686, 81302446,and 81703249).
文摘Background:The Shanghai growth standards are higher than World Health Organization (WHO) growth standards,which may influence the feeding practices of the caregivers and increase the risk of overweight in these infants.This study aimed to compare the effects of different growth standards on childhood obesity in Shanghai metropolitan area. Methods:This was a cluster-randomized controlled trial conducted in 2 downtown areas with 19 community health service centers in Shanghai from November 2013 to December 2015.Randomization was done at the level of the community.Infants (health newborns) were assessed and monitored by the Shanghai growth standards (S-group) and the 2006 WHO growth standards (W-group),respectively.Measurements were taken at 1.0,2.0,4.0,6.0,9.0 and 12.0 months of age during follow-up period.Based on the values of length and weight measurements,according to the group’s growth standards,doctors provided the caregivers with corresponding clinical consultation.Changes in weight-for-age z-score (WAZ),length-for-age z-score (LAZ),and weight-for length z-score (WLZ) between 2 groups were assessed using mixed regression models.Overweight was compared between 2 group at all follow-up measurements. Results:A total of 6509 infants (52.1% were boys) were in the W-group,and 8510 infants (51.4% were boys) were in the S-group.The overweight ratios between two groups were distinct at 9 months of age (3.4% in W-group and 4.3% in S-group) and 12 months of age (2.2% in W-group and 3.8% in S-group),and the differences were statistically significant (P= 0.020 and P<0.001,respectively).Compared to W-group,the increase in WAZ (coefficient= 0.04,P=0.004) and WLZ (coefficient= 0.09,P <0.001) were significantly greater,and the LAZ was lower (coefficient = -0.04,P = 0.047) in S-group (W-group values were used as reference in mixed regression models). Conclusion:Compared to the Shanghai growth standards,the adoption of WHO 2006 growth standards would reduce the risk of infant overweight in Shanghai metropolitan area up to 1 year of age.
