Bone defects are common clinical problems in the world,and there are many biomaterials used for treat-ing them.However,there is still a paucity of bioactive materials capable of modulating the immune mi-croenvironment...Bone defects are common clinical problems in the world,and there are many biomaterials used for treat-ing them.However,there is still a paucity of bioactive materials capable of modulating the immune mi-croenvironment.Therefore,it is necessary to identify new therapeutic strategies to regulate the immune microenvironment of the bone defect to further promote osteogenesis.Hydroxyapatite(HAP)is an impor-tant mineral for the framework of the human body.Recently,HAP has become a key research object for bone tissue engineering applications due to its unique tailored properties and similarity to bone tissue.Here,we prepared rod-shaped HAP(rHAP)with different concentrations(0,100,200,and 300μg/mL).The slowly released Ca^(2+)of 200μg/mL rHAP can induce macrophage phenotype 2(M2)polarization to decrease inflammatory cytokine secretion via the PI3K-Akt and Wnt/β-catenin pathways.In addition,rHAP can induce osteogenesis through the osteogenic differentiation of rat bone marrow mesenchymal stem cells.In conclusion,the 200μg/mL rHAP shows the potential for osteoimmunomodulation in a bone defect in vitro and in vivo,which is beneficial to the treatment of bone defects.展开更多
基金This work was funded by the National Key Research and Development Program(Grant No.2020YFC2009004)the National Natural Science Foundation of China(Grant no.52172282)+1 种基金the China Postdoctoral Science Foundation(Grant No.2021M690106)the New clinical practical technology in the Medical Affairs Department of the Qilu Hospital of Shandong University(Grant No.2019-08).We thank the Translational Medicine Core Facility of Shandong University for their consultation and instruments that supported this work.
文摘Bone defects are common clinical problems in the world,and there are many biomaterials used for treat-ing them.However,there is still a paucity of bioactive materials capable of modulating the immune mi-croenvironment.Therefore,it is necessary to identify new therapeutic strategies to regulate the immune microenvironment of the bone defect to further promote osteogenesis.Hydroxyapatite(HAP)is an impor-tant mineral for the framework of the human body.Recently,HAP has become a key research object for bone tissue engineering applications due to its unique tailored properties and similarity to bone tissue.Here,we prepared rod-shaped HAP(rHAP)with different concentrations(0,100,200,and 300μg/mL).The slowly released Ca^(2+)of 200μg/mL rHAP can induce macrophage phenotype 2(M2)polarization to decrease inflammatory cytokine secretion via the PI3K-Akt and Wnt/β-catenin pathways.In addition,rHAP can induce osteogenesis through the osteogenic differentiation of rat bone marrow mesenchymal stem cells.In conclusion,the 200μg/mL rHAP shows the potential for osteoimmunomodulation in a bone defect in vitro and in vivo,which is beneficial to the treatment of bone defects.
