MicroRNA-126 inhibits the proliferation of lung cancer cell line A549

Objective:To study the role of microRNA-126 in the development of lung cancer.Methods:The biological function of microRNA-126 was delected using EdU assay and CCK-8 assay:the target gene of microRNA-126 was analyzed using real time RT-PCR and Western blot assay.Results:In A549 cell line,overexpression of microRNA-126 inhibils the prolileralion rate:VEGF is the target gene of microRNA-126:microRNA-126 exerts its function via regulating VEGF protein level.Conclusions:microRNA-126 inhibits the proliferation in A549 cell line.

Pancreatic perivascular epithelioid cell tumor: A case report with clinicopathological features and a literature review

Perivascular epithelioid cell tumor(PEComa) of the pancreas is an unusual tumor deriving from mesenchyma. This paper described a case of pancreatic PEComa, which was initially suspected as neuroendocrine carcinoma by biopsy, and therefore surgical treatment was recommended due to undetermined diagnosis. Examination of the surgical specimen under a microscope showed that the tumor cell's morphology was epithelioid or spindle-shaped, and ranged in a nested pattern. Additionally, these cells had a large extent of acidophilic cytoplasm, no mitotic figures, and expressed HMB-45, melan-p, and smooth muscle actin immunohistochemically. Pathological examination indicated that PEComa originated from the pancreas, but symptoms related to tuberous sclerosis were absent. Since PEComa is extremely rare in the pancreas, it is likely to be ignored in differential diagnosis. In conclusion, our article highlighted the clinicopathological features of PEComa, and we conducted a literature review focusing on PEComa so as to deepen the understanding of this tumor type.

Presentation of Boerhaave's syndrome as an upper-esophageal perforation associated with a right-sided pleural effusion:A case report

BACKGROUND Spontaneous esophageal rupture or Boerhaave’s syndrome is a rare and acute disease with a high incidence of misdiagnosis and mortality.Here,we aimed to explore the clinical characteristics,diagnosis,treatment,and prognosis of spontaneous esophageal rupture,and to analyze the causes of misdiagnosis during the treatment of spontaneous esophageal rupture.CASE SUMMARY The clinical features of the patient with spontaneous esophageal rupture misdiagnosed earlier as pleural effusion were retrospectively analyzed and the reasons for misdiagnosis are discussed based on a current review of the literature.The patient was admitted to a local hospital due to shortness of breath accompanied by vomiting and abdominal distension for five hours.Based on the computed tomography(CT)scan analysis,clinically,right pleural effusion was diagnosed.However,the patient was unwilling to undergo right closed thoracic drainage.The patient also had intermittent fevers against infection,and during the course of treatment,he complained of chest pain,following which,he was transferred to our hospital.Grapefruit-like residue drainage fluid was observed.Re-examination of the chest CT scans suggested the presence of spontaneous perforation in the upper left esophagus.Therefore,the patient underwent an urgent esophageal hiatus repair.Unfortunately,the patient died of infection and respiratory failure due to progressive dyspnea after surgery.CONCLUSION Spontaneous esophageal rupture is a rare disease associated with high fatality.The patients do not present typical clinical symptoms and the disease progresses rapidly.This case report highlights the importance of a dynamic review of chest CT scan,not only for the initial identification of segmental injury but also for prioritizing subsequent treatment strategies.Moreover,we have presented some clues for clinicians to recognize and diagnose spontaneous esophageal rupture at rare sites(upper-esophageal segment)through this case report of spontaneous esophageal rupture that caused the patient’s death.We have also summarized the reasons for the misdiagnosis and lessons learned.

Regional gravity survey and application in oil and gas exploration in China

Ground gravity survey for regional structure unit delineation and oil and gas exploration in China is addressed in this paper with examples. Gravity survey scales, coverage, technical regulations and achievements at the national level are introduced, including data processing and anomaly interpretation techniques. Bouguer anomalies of terrestrial territories of China and classification of anomalous feature zones are also described;they are well correlated with lithotectonical boundaries, fault zones, and unexposed igneous plutons. Recent study results of petroliferous sedimentary basins are presented as well, including concealed boundaries and major structures of large basins. It is concluded that gravity survey is the most effective and economic method in unveiling unexposed and deep-seated structures, targeting and delineating oil and gas-bearing sedimentary basins, and locating main trap structures within prolific basins in early stage of exploration in China. Suggestions for improving exploration of both conventional and unconventional oil and gas reservoirs in China are also given in the paper.

Designation and development of biomedical Ti alloys with finer biomechanical compatibility in long-term surgical implants

与优秀 biomechanical 相容性开发新钛合金是一个重要研究方向外科植入材料。现在的纸总结生物医学的钛合金的国际研究和开发。瞄准增加 biomechanical 相容性，它也介绍合金设计的探索和改进，机械处理，微观结构和阶段转变，并且以后最后为关于生物医学的钛合金的科学研究构画出方向。

An index method to evaluate growers' pesticide use for identifying on-farm innovations and effective alternative pest management strategies:a case study of winegrape in Madera County,California

Winegrape is an important perennial crop in California,USA.Each year California winegrape farming consumes about 20 million kilograms of pesticides that have been a pollutant source to the fresh water systems of the state.The variation of pesticide use among winegrape growers has been significant.It has been observed that some growers have developed effective ways to reduce pesticide use,yet control pests efficiently to ensure harvest.Iden-tification of the growers with low and high pesticide use is very helpful to extension programs that aim on reducing pesticide environmental risk.In this study,an index approach is proposed to quantitatively measure pesticide use intensity at grower level.An integrated pesticide use index is developed by taking pesticide quantity and toxicity into account.An additive formula and a multiplying formula were used to calculate the pesticide use index,i.e.,PUI and PUIM.It was found that both PUI and PUIM were capable of identifying the low and high pesticide users while PUI was slightly more conservative than PUIM.All pesticides used in California winegrape farming were taken into account for calculating the indices.Madera County,one of the largest winegrape producers in California,was taken as an example to test the proposed approach.In year 2000,among the total 208 winegrape growers,28 with PUI≤10 and 34 with 10<PUI≤20 were identified as low pesticide users who were characterized with both low quantity and low toxicity of pesticide use.Most of the growers had small-sized vineyards,i.e.,one field and small planted areas.Furthermore,they had very low pesticide use intensity,used only 1-2 types of pesticides(mainly fungicides) ,applied few pesticides(1-3 only) ,and emphasized the use of low toxicity compounds.Meanwhile,19 growers with PUI>60,identified as high pesticide users,had large-sized vineyards,i.e.,more fields and large planted areas.They used all types of pesticides and many compounds,which indicated that their pest controls heavily depended on pesticides rather than on-farm management.Through the case study,the proposed approach proved to be useful for analyzing the growers'pesticide use intensities and interpreting their pesticide use behaviors,which led to a new start point for further investigation of searching ways to reduce pesticide environmental risk.

Atherosclerotic renal artery stenosis: how should we do?

Atherosclerotic renal artery stenosis(ARAS)is a common form of renal vascular disease and progressive disease.It could lead to resistant hypertension and renal insufficiency.It is almost associated with ischemic nephropathy with progressive decline in renal function and cardiac complications including flash pulmonary edema.The presence of ARAS is also associated with an increased risk of cardiovascular death.Common therapeutic strategies for ARAS include medical therapy and angioplasty with stenting.