期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
New insights into the interactions between dark blue pigment from Vaccinium bracteatum Thunb leaves and digestive enzymes
1
作者 mingcong fan Zhengyu Bao +6 位作者 Tingting Li Jiajia Zhao Yan Li Haifeng Qian Hui Zhang Zhiming Rao Li Wang 《Food Bioscience》 SCIE 2022年第6期1856-1865,共10页
The dark blue pigment from Vaccinium bracteatum Thunb.leaves(VBTL)is a healthy ingredient for controlling postprandial blood glucose during the stage of dietary carbohydrate digestion.Our previous study has proved tha... The dark blue pigment from Vaccinium bracteatum Thunb.leaves(VBTL)is a healthy ingredient for controlling postprandial blood glucose during the stage of dietary carbohydrate digestion.Our previous study has proved that the dark blue pigment has an inhibition effect on pancreaticα-amylase.However,the influence on other digestive enzymes(α-glucosidases,trypsin,andα-chymotrypsin)is still unclear.Therefore,this research evaluated the impact of this dark blue pigment on the three digestive enzymes during in-vitro digestion.The effects of dark blue pigment on the activities and conformations of theα-glucosidase,trypsin,andα-chymotrypsin were confirmed by multi-spectroscopic techniques and molecular docking.The results showed that this pigment possessed a higher inhibition ability on theα-glucosidase.The complexes of three enzymes and the pigment all resulted in the changes in fluorescence quenching and secondary structures of these complexes.However,the interactions between two proteolytic enzymes and pigment slightly impacted their activities.Additionally,molecular docking suggested that the complex betweenα-glucosidase and pigment formed hydrogen bonds and hydrophobic interactions in the substrate-binding pocket.These findings provide creative insight into the VBTL dark blue pigment controlling postprandial hyperglycemia without side effects on the proteolytic enzymes. 展开更多
关键词 Vaccinium bracteatum Thunb.leaf Dark blue pigment α-glucosidase Spectrophotometric assays Molecular docking
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部