Quality Standard for Euonymi Grandiflorus Caulis and Folium

[Objectives]To establish the quality standard for Euonymi Grandiflorus Caulis and Folium.[Methods]Qualitative identification was performed by methods of origin identification,microscopic identification and thin layer chromatography(TLC);the moisture,total ash,acid-insoluble ash,and extract were detected using the method in the Chinese Pharmacopoeia(2020 Edition),and the contents of quercetin and kaempferol were determined by High Performance Liquid Chromatography(HPLC).[Results]The leaves of Euonymi Grandiflorus were subleathery,narrow and long elliptic or narrow obovate with cyme,and capsules were often with narrow wing ridge.Characteristics of microscopic identification were significant.Calcium oxalate cluster crystals,cubic crystals,inlaid parenchyma cells,starch granules,stomata,and fiber bundles could be observed.A TLC method for identification of Euonymi Grandiflorus Caulis and Folium was established.The contents of water,total ash,acid-insoluble ash were not higher than 13%,9%,12%,respectively;the extract were no less were not higher than 13%,9%,12%and 17%in ten batches of samples,respectively;the sum of quercetin and kaempferol were no less than 0.20%.[Conclusions]The quality standard of Euonymi Grandiflorus Caulis and Folium were established through the research.This method is accurate,specific,and reproducible and can be used for the quality control of Euonymi Grandiflorus Caulis and Folium.

Quality Standard of Qiang Herb Sophora davidii var.chuansiensis

[Objectives]To establish the quality standard of common clinical folk herb Sophora davidii var.chuansiensis.[Methods]The qualitative identification was performed by microscopic method and thin-layer chromatography(TLC).The contents of moisture,total ash,acid insoluble ash and extract were determined according to the methods of the Chinese Pharmacopoeia(2020).High performance liquid chromatography(HPLC)was used to determine the contents of oxymatrine and oxysophocarpine.[Results]The microscopic characteristics were obvious,including crystal sheath fiber,calcium oxalate square crystal,non glandular hair,stone cells,epidermal cells,stomata,cork cells,vessels with marginal pits and so on.TLC spots were clear,and the resolution was good.The contents of moisture,total ash,acid insoluble ash and extract from 10 batches of samples were 4.70%-8.33%,3.43%-4.19%,0.65%-1.02%and 14.67%-22.04%respectively.The determination results of oxymatrine were between 0.10%-0.33%,with an average value of 0.19%.The determination results of sophocarpine oxide were between 0.30%-0.38%,with an average value of 0.34%.[Conclusions]The established quality standard of S.davidii var.chuansiensis had good specificity and accuracy,and could be used for the quality control of herb S.davidii var.chuansiensis.