High salinity is one of the major limiting fac-tors that reduces crop productivity and quality.Herein,we report that small SALT TOLERANCE ENHANCER1(STE1)protein without any known conserved domains is required for toma...High salinity is one of the major limiting fac-tors that reduces crop productivity and quality.Herein,we report that small SALT TOLERANCE ENHANCER1(STE1)protein without any known conserved domains is required for tomato salt tolerance.Overexpression(OE)of SlSTE1 enhanced the tolerance to multiple chloride salts(NaCl,KCl,and LiCl)and oxidative stress,along with elevated antioxidant enzyme activities,increased abscisic acid(ABA)and chlorophyll contents,and reduced ma-londialdehyde(MDA)and reactive oxygen species(ROS)accumulations compared to that of wild-type(WT)plants.Moreover,decreased K^+efflux and increased H^+efflux were detected in the OE plants,which induced a higher K^+/Na^+ratio.In contrast,SlSTE1-RNAi plants dis-played decreased tolerance to salt stress.RNA-seq data revealed 1330 differentially expressed genes in the OE plants versus WT plants under salt stress,and the tran-scription of numerous and diverse genes encoding transcription factors,stress-related proteins,secondary metabolisms,kinases,and hormone synthesis/signaling-related proteins(notably ABA and 1-aminocyclopropane-1-carboxylate)was greatly elevated.Furthermore,SlSTE1-OE plants showed increased sensitivity to ABA,and the results suggest that SlSTE1 promotes ABA-dependent salt stress-responsive pathways by interacting with SlPYLs and SlSnRK2s.Collectively,our findings reveal that the small SlSTE1 protein confers salt tolerance via ABA sig-naling and ROS scavenging and improves ion homeo-stasis in tomato.展开更多
In eukaryotes,histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional activation.HDA6 is a histone deacetylase involved in the transcriptional regulation of...In eukaryotes,histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional activation.HDA6 is a histone deacetylase involved in the transcriptional regulation of genes and transposable elements(TEs)in Arabidopsis thaliana.HDA6 has been shown to participate in several complexes in plants,including a conserved SIN3 complex.Here,we uncover a novel protein complex containing HDA6,several Harbinger transposon-derived proteins(HHP1,SANT1,SANT2,SANT3,and SANT4),and MBD domain-containing proteins(MBD1,MBD2,and MBD4).We show that mutations of all four SANT genes in the sant-null mutant cause increased expression of the flowering repressors FLC,MAF4,and MAF5,resulting in a late flowering phenotype.Transcriptome deep sequencing reveals that while the SANT proteins and HDA6 regulate the expression of largely overlapping sets of genes,TE silencing is unaffected in sant-null mutants.Our global histone H3 acetylation profiling shows that SANT proteins and HDA6 modulate gene expression through deacetylation.Collectively,our findings suggest that Harbinger transposon-derived SANT domain-containing proteins are required for histone deacetylation and flowering time control in plants.展开更多
基金This work was supported by the National NaturalScience Foundation of China(31700226)and the Pri-ority Academic Program Development of JiangsuHigher Education lnstitutions(PAPD).
文摘High salinity is one of the major limiting fac-tors that reduces crop productivity and quality.Herein,we report that small SALT TOLERANCE ENHANCER1(STE1)protein without any known conserved domains is required for tomato salt tolerance.Overexpression(OE)of SlSTE1 enhanced the tolerance to multiple chloride salts(NaCl,KCl,and LiCl)and oxidative stress,along with elevated antioxidant enzyme activities,increased abscisic acid(ABA)and chlorophyll contents,and reduced ma-londialdehyde(MDA)and reactive oxygen species(ROS)accumulations compared to that of wild-type(WT)plants.Moreover,decreased K^+efflux and increased H^+efflux were detected in the OE plants,which induced a higher K^+/Na^+ratio.In contrast,SlSTE1-RNAi plants dis-played decreased tolerance to salt stress.RNA-seq data revealed 1330 differentially expressed genes in the OE plants versus WT plants under salt stress,and the tran-scription of numerous and diverse genes encoding transcription factors,stress-related proteins,secondary metabolisms,kinases,and hormone synthesis/signaling-related proteins(notably ABA and 1-aminocyclopropane-1-carboxylate)was greatly elevated.Furthermore,SlSTE1-OE plants showed increased sensitivity to ABA,and the results suggest that SlSTE1 promotes ABA-dependent salt stress-responsive pathways by interacting with SlPYLs and SlSnRK2s.Collectively,our findings reveal that the small SlSTE1 protein confers salt tolerance via ABA sig-naling and ROS scavenging and improves ion homeo-stasis in tomato.
基金This work was supported by grants from the National Key Research and Development Program of China(2020YFE0202300)the Central Public-interest Scientific Institution Basal Research Fund,the BBSRC under the Grant Reference BB/P008569/1 to J.G.C.N.V.and E.dL.,and an Erasmus plus training award to L.G.
文摘In eukaryotes,histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional activation.HDA6 is a histone deacetylase involved in the transcriptional regulation of genes and transposable elements(TEs)in Arabidopsis thaliana.HDA6 has been shown to participate in several complexes in plants,including a conserved SIN3 complex.Here,we uncover a novel protein complex containing HDA6,several Harbinger transposon-derived proteins(HHP1,SANT1,SANT2,SANT3,and SANT4),and MBD domain-containing proteins(MBD1,MBD2,and MBD4).We show that mutations of all four SANT genes in the sant-null mutant cause increased expression of the flowering repressors FLC,MAF4,and MAF5,resulting in a late flowering phenotype.Transcriptome deep sequencing reveals that while the SANT proteins and HDA6 regulate the expression of largely overlapping sets of genes,TE silencing is unaffected in sant-null mutants.Our global histone H3 acetylation profiling shows that SANT proteins and HDA6 modulate gene expression through deacetylation.Collectively,our findings suggest that Harbinger transposon-derived SANT domain-containing proteins are required for histone deacetylation and flowering time control in plants.
