With the advent of the era of short video,people can receive more information easy to be read and understood in a short time.Meteorological communicators should seize this opportunity,dig out characteristics and make ...With the advent of the era of short video,people can receive more information easy to be read and understood in a short time.Meteorological communicators should seize this opportunity,dig out characteristics and make innovations.In this paper,the first award-winning excellent short weather video in Hubei Province is taken as an example,to analyze some characteristics of short weather video,and some innovative development strategies are proposed.展开更多
Insects first began evolving hundreds of millions of years ago,and aided by gut microbes,they have been consuming hydrocarbon polymers ever since.Few man-made plastic polymers are chemically novel,so it is reasonable ...Insects first began evolving hundreds of millions of years ago,and aided by gut microbes,they have been consuming hydrocarbon polymers ever since.Few man-made plastic polymers are chemically novel,so it is reasonable that insect/microbe systems can be found or developed to degrade them rapidly.However,remediation of global plastic waste problems should involve more than just conversion into CO_(2).Some industryscale microbial enzymatic degradation of plastic polymers may yield valuable monomers,but the plastic waste starting material must be of uniform chemistry and clean.This adds cost to the process.Many insect species can be utilized for animal feed as well as human food.Some of these insects have the capability to degrade plastic polymers.However,valorizing plastic wastes by producing edible insects or useful frass has largely been overlooked.Here we assemble the current knowledge of plastic degradation rates by insects.In addition,we also show the first instance of insect degradation of polyurethane and the first identification and isolation of insect gut fungi as directly aiding insect degradation.展开更多
Dihydropyran(DHP)compounds are not only found in natural products and bioactive molecules,but also serve as important precursors in organic synthesis.Nonetheless,traditional methods for the construction of such compou...Dihydropyran(DHP)compounds are not only found in natural products and bioactive molecules,but also serve as important precursors in organic synthesis.Nonetheless,traditional methods for the construction of such compounds are usually limited to disubstituted DHPs.To address this synthetic challenge,reported here is an efficient electrochemical strategy toward the selenated and trifluoromethylated DHP compounds.The reaction proceeded smoothly under mild electrolysis conditions.The broad substrate scope(>50 examples)and scalable synthesis demonstrated the complexity-building potential of the strategy.Initial mechanistic studies reveal that cyclization may involve a radical process.This protocol may promote the further development of diversified synthesis of multi-substituted dihydropyran.展开更多
Sensitive and specific imaging of tumor-associated biomarkers offers an effective way for cancer diagnosis.However,most of the reported imaging probes are limited to the targeting of a single biomarker,which may impai...Sensitive and specific imaging of tumor-associated biomarkers offers an effective way for cancer diagnosis.However,most of the reported imaging probes are limited to the targeting of a single biomarker,which may impair imaging precision.Here,we report an aptamer-based,AND-gate DNA nanodevice that relies on the sequential processing of two tumor-associated proteins to produce a specific fluorescent signal for precise tumor imaging.The DNA nanodevice integrates an AS1411 aptamer to bind cell surface nucleolin for tumor targeting and a DNA sensor to respond to intracellular apurinic/apyrimidinic endonuclease 1 for activatable imaging.We evaluated the performance of the system in both living cells and a tumor-bearing mouse model,and demonstrated its capability in tumor cell-specific imaging.We anticipate that this strategy will accelerate the design of multivariate protein-stimulated probes for tumor diagnosis.展开更多
The normal operation of lysosome, mitochondria, Golgi apparatus and endoplasmic reticulum plays a significant role in maintaining cell homeostasis. Reflecting the state and function of lysosomes, viscosity is a pivota...The normal operation of lysosome, mitochondria, Golgi apparatus and endoplasmic reticulum plays a significant role in maintaining cell homeostasis. Reflecting the state and function of lysosomes, viscosity is a pivotal parameter to assess the stability of microenvironment. Herein, based on TICT mechanism,a new NIR pH-dependent fluorescent probe DCIC with push-pull electronic moiety was synthesized to identify the lysosomes viscosity. In viscous media, DCIC was highly sensitive to viscosity, fluorescence intensity increased by 180 times as viscosity increased from 1.0 cp to 438.4 cp. In addition, DCIC have high localization ability for lysosome, mitochondria, Golgi apparatus, and endoplasmic reticulum and can monitor lysosomal viscosity fluctuations with laser confocal microscopy.展开更多
Based on 4-bromo-1,8-naphthalic anhydride,one novel ratiometric fluorescence H_(2)S-probe(IDNA)was designed and synthesized.Further studies indicate that IDNA can sensitively recognize H_(2)S(detection limit of 7 μmo...Based on 4-bromo-1,8-naphthalic anhydride,one novel ratiometric fluorescence H_(2)S-probe(IDNA)was designed and synthesized.Further studies indicate that IDNA can sensitively recognize H_(2)S(detection limit of 7 μmol/L)with good selectivity and anti-interference ability.In addition,IDNA has satisfactory photostability in HeLa cells,ability of mitochondrial co-localization,and can be utilized in fluorescence imaging of H_(2)S.展开更多
It is significant for cell physiology to keep the homeostasis of p H, and it is highly demanded to develop ratiometric fluorescent sensors toward p H. In this work, under mild condition, through the electrostatic inte...It is significant for cell physiology to keep the homeostasis of p H, and it is highly demanded to develop ratiometric fluorescent sensors toward p H. In this work, under mild condition, through the electrostatic interaction between carbon nanodots（CDs） and organic molecules, two novel ratiometric fluorescence hybrid nanosensors were fabricated for sensing acidic p H. These nanohybrid systems possess dual emission peaks at 455 and 527 nm under a single excitation wavelength of 380 nm in acidic p H condition.With the increasing of p H, the fluorescence of the 1,8-naphthalimide derivative completely quenches,while the blue fluorescence of CDs keeps constant. Furthermore, the CDsàorganic molecular nanohybrids exhibit excellent anti-disturbance ability, reversible p H sensing ability, and a linear response range in wide p H range respectively. Besides the ability to target lysosome, with one of the nanosensor, stimulated p H change has been successfully tracked in a ratiometric manner via fluorescence imaging.展开更多
A ratiometric fluorescent hybrid nanoprobe CDs-1 for arginine(Arg),exhibiting high sensitivity(the limit of detection,LOD,being 6.5×10^-8 mol/L) and excellent selectivity and anti-interference ability,was fabrica...A ratiometric fluorescent hybrid nanoprobe CDs-1 for arginine(Arg),exhibiting high sensitivity(the limit of detection,LOD,being 6.5×10^-8 mol/L) and excellent selectivity and anti-interference ability,was fabricated through fluorescence resonance energy transfer(FRET) and the electrostatic attraction between positively-charged hemicyanine molecules and negatively-charged carbon dots(CDs).Arg can be quantitatively detected in the concentration range from 6.0×10^-5 mol/L to 2.7×10^-4 mol/L.Further,due to its ability to target mitochondrion and low cytotoxicity,intracellular Arg was succes s fully tracked through ratiometric fluorescence imaging.展开更多
The utilization of economic and practical trifluoromethyl reagents to introduce trifluoromethyl(CF_(3))groups into organic molecules is an important research focus due to their role as vital structuralmotifs in the ph...The utilization of economic and practical trifluoromethyl reagents to introduce trifluoromethyl(CF_(3))groups into organic molecules is an important research focus due to their role as vital structuralmotifs in the pharmaceutical and agricultural industries.Herein,we disclose a cost-efficient and practical approach of photoinduction of a radical relay process for the synthesis ofα-CF_(3)ketones.We showed that trifluoromethylation reagents such as TES-OTf,Tf2O,TMS-OTf,TBS-OTf,H-OTf,and others,could be used successfully as inexpensive CF_(3)precursors under metal-free and redox-neutral conditions.The potential application of this protocol was highlighted further by late-state modification of drug segments and gram-scale synthesis.Our mechanistic studies revealed that this transformation might involve a radical process,and acetic acid(CH3COOH)not only promoted the formation of enol triflates,but also accelerated the in situ conversion from enol triflates toα-CF_(3)ketones.展开更多
文摘With the advent of the era of short video,people can receive more information easy to be read and understood in a short time.Meteorological communicators should seize this opportunity,dig out characteristics and make innovations.In this paper,the first award-winning excellent short weather video in Hubei Province is taken as an example,to analyze some characteristics of short weather video,and some innovative development strategies are proposed.
基金Key Research Program of the Ministry of Sciences and Technology(Grant No.2017YFC0505101)of ChinaChinese Academy of Sciences,President’s International Fellowship Initiative(CAS-PIFI),Grant No.2019PC0011,2017PC0035+7 种基金Key Research Program of Frontier Sciences,CAS,Grant No.QYZDY-SSW-SMC014We thank the National Science Foundation of China(NSFC)for funding this work under the project codes Y4ZK111B01,41761144055,3181101433,41771063,31650410651,41761144055 and 31550110215We are thankful to Zhijia Gu,Key Laboratories for Plant Diversity and Biogeography of East China,Kunming Institute of Botany,Chinese Academy of Sciences for scanning electron microscopy.G.G.O.Dossa thanks China Postdoctoral Foundation Grant No.2017M613021the young international staff Chinese Academy of Sciences(CAS)president international fellowship initiative(PIFI)grants#2019FYB0001 and 2017PC0035Heng Gui would thank the CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows(Grant No.2017LH029)the China Postdoctoral Science Foundation(Grant No.2018M633435)the 2018 Yunnan Province Postdoctoral Science Research Foundation.Heng Gui would also like to thank the support from the Human Resources and Social Security Department of Yunnan Province,German Academic Exchange Service(DAAD)under the program:Research Stays for University Academics and Scientists,2018(Ref.No.91691203)the China Scholarship Council under the State Scholarship Fund(Ref.No.201804910259).
文摘Insects first began evolving hundreds of millions of years ago,and aided by gut microbes,they have been consuming hydrocarbon polymers ever since.Few man-made plastic polymers are chemically novel,so it is reasonable that insect/microbe systems can be found or developed to degrade them rapidly.However,remediation of global plastic waste problems should involve more than just conversion into CO_(2).Some industryscale microbial enzymatic degradation of plastic polymers may yield valuable monomers,but the plastic waste starting material must be of uniform chemistry and clean.This adds cost to the process.Many insect species can be utilized for animal feed as well as human food.Some of these insects have the capability to degrade plastic polymers.However,valorizing plastic wastes by producing edible insects or useful frass has largely been overlooked.Here we assemble the current knowledge of plastic degradation rates by insects.In addition,we also show the first instance of insect degradation of polyurethane and the first identification and isolation of insect gut fungi as directly aiding insect degradation.
基金supported by the National Key R&D Program of China(2022YFA1505100,2021YFA1500100)the National Natural Science Foundation of China(22031008)+1 种基金the Fundamental Research Funds for the Central Universities(2042022rc0030,2042023kf0108,2042023kf1002)the Science Foundation of Wuhan(2020010601012192)。
文摘Dihydropyran(DHP)compounds are not only found in natural products and bioactive molecules,but also serve as important precursors in organic synthesis.Nonetheless,traditional methods for the construction of such compounds are usually limited to disubstituted DHPs.To address this synthetic challenge,reported here is an efficient electrochemical strategy toward the selenated and trifluoromethylated DHP compounds.The reaction proceeded smoothly under mild electrolysis conditions.The broad substrate scope(>50 examples)and scalable synthesis demonstrated the complexity-building potential of the strategy.Initial mechanistic studies reveal that cyclization may involve a radical process.This protocol may promote the further development of diversified synthesis of multi-substituted dihydropyran.
基金financially supported by the Beijing Natural Science Foundation(JQ20005).
文摘Sensitive and specific imaging of tumor-associated biomarkers offers an effective way for cancer diagnosis.However,most of the reported imaging probes are limited to the targeting of a single biomarker,which may impair imaging precision.Here,we report an aptamer-based,AND-gate DNA nanodevice that relies on the sequential processing of two tumor-associated proteins to produce a specific fluorescent signal for precise tumor imaging.The DNA nanodevice integrates an AS1411 aptamer to bind cell surface nucleolin for tumor targeting and a DNA sensor to respond to intracellular apurinic/apyrimidinic endonuclease 1 for activatable imaging.We evaluated the performance of the system in both living cells and a tumor-bearing mouse model,and demonstrated its capability in tumor cell-specific imaging.We anticipate that this strategy will accelerate the design of multivariate protein-stimulated probes for tumor diagnosis.
基金financial supports from Scientific and Technological Key Project in Henan Province(No.22170015)National Natural Science Foundation of China(No.U1704161)+1 种基金Zhengzhou University(No.32211807)Henan Provincial Science and Technology Research Project(No.JC21253010)。
文摘The normal operation of lysosome, mitochondria, Golgi apparatus and endoplasmic reticulum plays a significant role in maintaining cell homeostasis. Reflecting the state and function of lysosomes, viscosity is a pivotal parameter to assess the stability of microenvironment. Herein, based on TICT mechanism,a new NIR pH-dependent fluorescent probe DCIC with push-pull electronic moiety was synthesized to identify the lysosomes viscosity. In viscous media, DCIC was highly sensitive to viscosity, fluorescence intensity increased by 180 times as viscosity increased from 1.0 cp to 438.4 cp. In addition, DCIC have high localization ability for lysosome, mitochondria, Golgi apparatus, and endoplasmic reticulum and can monitor lysosomal viscosity fluctuations with laser confocal microscopy.
基金We are grateful for the financial supports from the National Natural Science Foundation of China(Nos.U1704161,U1504203,21601158)Zhengzhou University(No.32210431).
文摘Based on 4-bromo-1,8-naphthalic anhydride,one novel ratiometric fluorescence H_(2)S-probe(IDNA)was designed and synthesized.Further studies indicate that IDNA can sensitively recognize H_(2)S(detection limit of 7 μmol/L)with good selectivity and anti-interference ability.In addition,IDNA has satisfactory photostability in HeLa cells,ability of mitochondrial co-localization,and can be utilized in fluorescence imaging of H_(2)S.
基金supports from National Natural Science Foundation of China (Nos. 21601158, U1504203 and J1210060)Key Laboratory of Photochemical Conversion and Optoelectronic Materials and Zhengzhou University
文摘It is significant for cell physiology to keep the homeostasis of p H, and it is highly demanded to develop ratiometric fluorescent sensors toward p H. In this work, under mild condition, through the electrostatic interaction between carbon nanodots（CDs） and organic molecules, two novel ratiometric fluorescence hybrid nanosensors were fabricated for sensing acidic p H. These nanohybrid systems possess dual emission peaks at 455 and 527 nm under a single excitation wavelength of 380 nm in acidic p H condition.With the increasing of p H, the fluorescence of the 1,8-naphthalimide derivative completely quenches,while the blue fluorescence of CDs keeps constant. Furthermore, the CDsàorganic molecular nanohybrids exhibit excellent anti-disturbance ability, reversible p H sensing ability, and a linear response range in wide p H range respectively. Besides the ability to target lysosome, with one of the nanosensor, stimulated p H change has been successfully tracked in a ratiometric manner via fluorescence imaging.
基金National Natural Science Foundation of China(Nos.U1704161,U1504203,21601158)Zhengzhou University(No.32210431)for the financial supports。
文摘A ratiometric fluorescent hybrid nanoprobe CDs-1 for arginine(Arg),exhibiting high sensitivity(the limit of detection,LOD,being 6.5×10^-8 mol/L) and excellent selectivity and anti-interference ability,was fabricated through fluorescence resonance energy transfer(FRET) and the electrostatic attraction between positively-charged hemicyanine molecules and negatively-charged carbon dots(CDs).Arg can be quantitatively detected in the concentration range from 6.0×10^-5 mol/L to 2.7×10^-4 mol/L.Further,due to its ability to target mitochondrion and low cytotoxicity,intracellular Arg was succes s fully tracked through ratiometric fluorescence imaging.
基金supported by the National Natural Science Foundation of China(22031008)Science Foundation of Wuhan(2020010601012192)The Program of Introducing Talents of Discipline to Universities of China(111 Program)is also appreciated。
基金This work was supported by the National Natural Science Foundation of China(nos.21390402 and 21520102003)the Hubei Province Natural Science Foundation of China(no.2017CFA010)The Program of Introducing Talents of Discipline to Universities of China(111 Program)is also appreciated.
文摘The utilization of economic and practical trifluoromethyl reagents to introduce trifluoromethyl(CF_(3))groups into organic molecules is an important research focus due to their role as vital structuralmotifs in the pharmaceutical and agricultural industries.Herein,we disclose a cost-efficient and practical approach of photoinduction of a radical relay process for the synthesis ofα-CF_(3)ketones.We showed that trifluoromethylation reagents such as TES-OTf,Tf2O,TMS-OTf,TBS-OTf,H-OTf,and others,could be used successfully as inexpensive CF_(3)precursors under metal-free and redox-neutral conditions.The potential application of this protocol was highlighted further by late-state modification of drug segments and gram-scale synthesis.Our mechanistic studies revealed that this transformation might involve a radical process,and acetic acid(CH3COOH)not only promoted the formation of enol triflates,but also accelerated the in situ conversion from enol triflates toα-CF_(3)ketones.
