Since alkaloids are the major active constituents of Rhizoma corydalis(RC),a convenient and accurate analytical method is needed for their identification and characterization.Here we report a method to profile the alk...Since alkaloids are the major active constituents of Rhizoma corydalis(RC),a convenient and accurate analytical method is needed for their identification and characterization.Here we report a method to profile the alkaloids in RC based on liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(LC–Q-TOF-MS/MS).A total of 16 alkaloids belonging to four different classes were identified by comparison with authentic standards.The fragmentation pathway of each class of alkaloid was clarified and their differences were elucidated.Furthermore,based on an analysis of fragmentation pathways and alkaloid profiling,a rapid and accurate method for the identification of unknown alkaloids in RC is proposed.The method could also be useful for the quality control of RC.展开更多
基金The work was funded by the National Natural Science Foundation of China(Nos.81202950 and 81102795)National Key Project of the Scientific and Technical Supporting Programs of China(No.2012ZX10004301-608).
文摘Since alkaloids are the major active constituents of Rhizoma corydalis(RC),a convenient and accurate analytical method is needed for their identification and characterization.Here we report a method to profile the alkaloids in RC based on liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(LC–Q-TOF-MS/MS).A total of 16 alkaloids belonging to four different classes were identified by comparison with authentic standards.The fragmentation pathway of each class of alkaloid was clarified and their differences were elucidated.Furthermore,based on an analysis of fragmentation pathways and alkaloid profiling,a rapid and accurate method for the identification of unknown alkaloids in RC is proposed.The method could also be useful for the quality control of RC.
