TLC Identification of Schisandra Chinensis Liquid Extract and Antler Essence in Qianglinaoqingsu Tablets

[Objectives]To study the quality standard of Chinese drug in compound Qianglinaogingsu Tablets(Fructus Schisandrae Chinensis and Coru Cervi Pantotrichum).[Methods]The thin layer chromatography(TLC)differentiation method was established for Fructus Schisandrae Chinensis and Cornu Cervi Pantotrichum.[Results]TLC spots developed were fairly clear,and the blank test showed no interference.[Conclusions]The method is fast,simple and can be used for quality control of Qianglinaoqingsu Tablets.

Differentiation of mesenchymal stem cells into neuronal cells on fetal bovine acellular dermal matrix as a tissue engineered nerve scaffold

The purpose of this study was to assess fetal bovine acellular dermal matrix as a scaffold for supporting the differentiation of bone marrow mesenchymal stem cells into neural cells fol-lowing induction with neural differentiation medium. We performed long-term, continuous observation of cell morphology, growth, differentiation, and neuronal development using several microscopy techniques in conjunction with immunohistochemistry. We examined speciifc neu-ronal proteins and Nissl bodies involved in the differentiation process in order to determine the neuronal differentiation of bone marrow mesenchymal stem cells. The results show that bone marrow mesenchymal stem cells that differentiate on fetal bovine acellular dermal matrix display neuronal morphology with unipolar and bi/multipolar neurite elongations that express neuro-nal-speciifc proteins, includingβIII tubulin. The bone marrow mesenchymal stem cells grown on fetal bovine acellular dermal matrix and induced for long periods of time with neural differen-tiation medium differentiated into a multilayered neural network-like structure with long nerve ifbers that was composed of several parallel microifbers and neuronal cells, forming a complete neural circuit with dendrite-dendrite to axon-dendrite to dendrite-axon synapses. In addition, growth cones with filopodia were observed using scanning electron microscopy. Paraffin sec-tioning showed differentiated bone marrow mesenchymal stem cells with the typical features of neuronal phenotype, such as a large, round nucleus and a cytoplasm full of Nissl bodies. The data suggest that the biological scaffold fetal bovine acellular dermal matrix is capable of supporting human bone marrow mesenchymal stem cell differentiation into functional neurons and the subsequent formation of tissue engineered nerve.

Output Feedback Robust H_(∞) Control for Discrete 2D Switched Systems

The two-dimensional(2-D)system has a wide range of applications in different fields,including satellite meteorological maps,process control,and digital filtering.Therefore,the research on the stability of 2-D systems is of great significance.Considering that multiple systems exist in switching and alternating work in the actual production process,but the system itself often has external perturbation and interference.To solve the above problems,this paper investigates the output feedback robust H_(∞)stabilization for a class of discrete-time 2-D switched systems,which the Roesser model with uncertainties represents.First,sufficient conditions for exponential stability are derived via the average dwell time method,when the system’s interference and external input are zero.Furthermore,in the case of introducing the external interference,the weighted robust H_(∞)disturbance attenuation performance of the underlying system is further analyzed.An output feedback controller is then proposed to guarantee that the resulting closed-loop system is exponentially stable and has a prescribed disturbance attenuation levelγ.All theorems mentioned in the article will also be given in the form of linear matrix inequalities(LMI).Finally,a numerical example is given,which takes two uncertain values respectively and solves the output feedback controller’s parameters by the theorem proposed in the paper.According to the required controller parameter values,the validity of the theorem proposed in the article is compared and verified by simulation.

Hybrid optical parametrically-oscillating emitter at 1930 nm for volumetric photoacoustic imaging of water content

Water plays a vital role in biological metabolism and it would be essential to trace the water content non-invasively,such as leveraging the vibrational absorption peak of the O-H bond.However,due to the lack of an efficient laser source,it was challenging to image the water content in the deep tissue with micron-level spatial resolution.To address this problem,we develop a high-power hybrid optical parametrically-oscillating emitter(HOPE)at 1930 nm,at which the vibrational absorption peak of the O-H bond locates.The maximum pulse energy is over 1.74μJ with a pulse repetition rate of 50 kHz and a pulse width of 15 ns.We employ this laser source in the optical-resolution photoacoustic microscopy(OR-PAM)system to image the water content in the phantom and the biological tissue in vitro.Our 1930-nm OR-PAM could map the water content in the complex tissue environment at high spatial resolution,deep penetration depth,improved sensitivity,and suppressed artifact signal of the lipid.

Lensless ultrafast optical imaging

Lensless single-shot ultrafast optical imaging is realized by integrating an acousto-optic programmable dispersive filter with spectrally filtered sequentially time all-optical mapping photography,which enables independent control of frame rate,frame intensity,and exposure time with a simple system design.