Anthocyanins,a categories of metabolites derived from flavonoid biosynthesis pathway,are responsible for the red color of peach.Anthocyanin metabolism is modulated by a complicated regulatory network.In this study,our...Anthocyanins,a categories of metabolites derived from flavonoid biosynthesis pathway,are responsible for the red color of peach.Anthocyanin metabolism is modulated by a complicated regulatory network.In this study,our results demonstrated that PpNAC25 was a component of anthocyanin regulatory network in peach.PpNAC25 showed a closed relationship with the well-known ripening-related gene NOR(no-ripening)and was highly expressed in peach fruit.Overexpression of PpNAC25 in poplar resulted in a redder shoot tips compared with EV control.PpNAC25 overexpression upregulated the anthocyanin biosynthetic and transportation genes in transcript levels and then increased anthocyanin contents.In Y1H and Luc/Ren assay,PpNAC25 bound to the promoter of the anthocyanin-activator PpMYB10.1 and PpMYB10.2 and activated its transcript.Moreover,PpNAC25 formed a heterodimer with PpNAC1,an anthocyanin-regulator.Our researches provide evidence that PpNAC25 may be a positive regulator of anthocyanin biosynthesis in peach fruit.展开更多
基金The work was conducted at the Henan Provincial Key Laboratory of Fruit and Cucurbit Biology and supported by the Joint Funds of the National Natural Science Foundation of China(U1804114)the Henan Province Outstanding Foreign Scholar Program(GZS2020007)the Modern Agricultural Industry Technology of Henan Province(S2014-11-G02).
文摘Anthocyanins,a categories of metabolites derived from flavonoid biosynthesis pathway,are responsible for the red color of peach.Anthocyanin metabolism is modulated by a complicated regulatory network.In this study,our results demonstrated that PpNAC25 was a component of anthocyanin regulatory network in peach.PpNAC25 showed a closed relationship with the well-known ripening-related gene NOR(no-ripening)and was highly expressed in peach fruit.Overexpression of PpNAC25 in poplar resulted in a redder shoot tips compared with EV control.PpNAC25 overexpression upregulated the anthocyanin biosynthetic and transportation genes in transcript levels and then increased anthocyanin contents.In Y1H and Luc/Ren assay,PpNAC25 bound to the promoter of the anthocyanin-activator PpMYB10.1 and PpMYB10.2 and activated its transcript.Moreover,PpNAC25 formed a heterodimer with PpNAC1,an anthocyanin-regulator.Our researches provide evidence that PpNAC25 may be a positive regulator of anthocyanin biosynthesis in peach fruit.
