Effect of the cyclic stretch and role of related signal pathway on the osteogenic differentiation of MC3T3-E1 cells

Mechanical strain stimulation is one of the important factors to regulate bone metabolism,maintain bone mass and bone structure.In the process of osteoblasts response to the mechanical strain stimulation,the signal transduction pathways were activated by mechanical stimulation Extracellular signal regulated kinase 1/2(ERK1/2)and Nuclear factor kappa B(NF-κB)signal pathways are both important in the process of signal transduction in osteoblasts.They played an important role in differentiation of osteogenic progenitor cells.Therefore in this research we cultured MC3T3-E1 osteoblasts,and established the vitro model of the mechanical strain stimulation,to observe the differentiation of MC3T3-E1 osteoblastic cells and investigate the interac-

Control Effects of Four Kinds of Insecticides against Empoasca vitis Gothe

Field test results showed that 24% Chlorfenapyr SC and 15% Indoxacarb EC had quick availability and long duration against Empoasca vitis Gothe. The control effects of 450 mL/hm2 24% Chlorfenapyr SC and 330 mL/hm2 15% Indoxacarb were significantly higher than that of 450 g/hm2 10% Imidacloprid WP ,which were ideal pesticides against E. vitis and could be popularized and applied in large area.

Risk Evaluation of Rainstorm and Flood in the Upper Reaches of Hunhe River (Qingyuan Section) Based on the FloodArea Model

By using data of hourly rainfall with all of the meteorological stations in the upper reaches of Hunhe River (Qingyuan Section), the digital elevation model, the land use and the disasters data etc., the storm flood process which happened on 16 August 2013 is reproduced simulated based on the rainstorm flooding of FloodArea model, also the flooding simulation and its effectiveness have been carried out. Conclusions are drawn as follows: there is a sharp rise of the flood depth falling behind the change of rainfall three or four hours. As the accumulation of the precipitation, the water level has increased, and when the precipitation process stops, the flood pooled into midstream and downstream gradually. According to the disaster investigation, in both the aspects of flooding scope and flooding depth of warning spots, the simulation result of FloodArea model agrees with the fact. It indicates that the FloodArea model generates good simulation effect in the upper reaches of Hunhe River (Qingyuan Section). Combined with population and GDP information, in the whole river, about eighty-three thousand people were affected by flooding, also one billion and five hundred seventy five million RMB of gross domestic product was lost. When the flood depth deepens and the flooding scope increases, correspondingly the loss rate of population and GDP rises and the flood risk increases.

Risk Assessment and Simulation on Storm Flood of the 100-Year Return Period in Hunhe River Basin

Based on the meteorological and geographic information data, with statistical method and the FloodArea model, the extreme daily rainfall of the 100-year return period in Hunhe River basin was established, through the simulation of rainstorm and flood disaster, characteristics of flood depth in warning spot Cangshi village in the upstream of the river were analysed, and possible effect on community economy was also evaluated. Results showed that, the precipitation of 100-year return period occurred, the flood depth has been below 1.0 meter in the most areas of Hunhe River basin, the depth was between 1.0 meter and 2.5 meters in the part areas of Hunhe River basin, and the flood depth has been exceed 2.5 meters in a small part of Hunhe River basin. After the beginning of precipitation, the flood was concentrated in the upper reaches of the river. With the accumulation of precipitation and the passage of time, the flood pools into midstream and downstream. Precipitation lasted for 24 hours, the warning spot was flooded in the beginning of precipitation. With the accumulation of precipitation, water level of the river increases gradually. The depth of warning spot has passed 1.0 meter at the 07 time of the whole process, and the maximum value of flood depth at warning spot was 1.083 meters that occurred at the 19 time. The flood depth of warning spot decreased gradually after the precipitation stopping, and the depth has been below 0.2 meters, the flood of upstream ended. Up to the end of the upstream flood process, in the whole river, about one million five hundred and sixty thousand people were affected by flooding, and thirty-eight billion and two hundred million RMB of gross domestic product were lost, in addition, dry land and paddy field were affected greatly, but woodland and grassland were less affected.

Onset velocity of circulating fluidization and particle residence time distribution:A CFD-DEM study

Until now, the onset velocity of circulating fluidization in liquid-solid fluidized beds has been defined by the turning point of the time required to empty a bed of particles as a function of the superfcial liquid velocity, and is reported to be only dependent on the liquid and particle properties. This study presents a new approach to calculate the onset velocity using CFD-DEM simulation of the particle residence time distribution （RTD）. The onset velocity is identified from the intersection of the fitted lines of the particle mean residence time as a function of superficial liquid velocity. Our results are in reasonable agreement with experimental data. The simulation indicates that the onset velocity is infuenced by the density and size of particles and weakly affected by riser height and diameter, A power-law function is proposed to correlate the mean particle residence time with the superficial liquid velocity. The collisional parameters have a minor effect on the mean residence time of particles and the onset velocity, but influence the particle RTD, showing some humps and trailing. The particle RTD is found to be related to the particle trajectories, which may indicate the complex flow structure and underlying mechanisms of the particle RTD.

Clustering behavior of solid particles in two-dimensional liquid-solid fluidized-beds

In this paper, the clustering behavior of solid particles in a two-dimensional （2D） liquid-solid fluidized-bed was studied by using the charge coupled devices （CCD） imaging measuring and processing technique and was characterized by fractal analysis. CCD images show that the distribution of solid particles in the 2D liquid-solid fluidised-bed is not uniform and self-organization behavior of solid particles was observed under the present experimental conditions. The solid particles move up in the 2D fluidized-bed in groups or clusters whose configurations are often in the form of horizontal strands. The box fractal dimension of the cluster images in the 2D liquid-solid fluidized-bed increases with the rising of solid holdup and reduces with the increment of solid particle diameter and superficial liquid velocity. At given solid holdup and solid particle size, the lighter particles show smaller fractal dimensions.

Hydrodynamic behavior of liquid-solid micro-fluidized beds determined from bed expansion

The bed-expansion characteristics of liquid-solid micro-fluidized beds were experimentally studied. Bed columns with inner diameters of 0.8, 1.45, and 2.3 mm were fabricated based on capillaries. Five parti- cle sizes in a range of 22-58 t^m were investigated. Bed-expansion curves were plotted using visually recorded bed-expansion heights. The bed expansion and initial fluidization behavior were compared with predictions for conventional-scale beds, Evident differences are reflected in lower expansion ratios and higher minimum fluidization velocities for micro-fluidized beds. These were attributed to the increase in the internal surface area of the particle beds and specific surface area of wall contact. The wall effect for micro-fluidized beds at higher particle/bed diameter ratios caused higher local voidage and an increase in expansion ratio. Correlations for the exponent and proportional coefficient in the Richardson-Zaki equation for micro-fluidized beds were proposed. The minimum fluidization velocities were correlated using a modification of the Ergun equation.

Experimental investigation of hydrodynamics of liquid-solid mini-fluidized beds

Expanded fluidization behavior in liquid-solid mini-fluidized beds （MFBs） was experimentally investigated using visual measurements. Wall effects in the liquid-solid MFBs were identified and explained. The measured incipient]minimum fluidization liquid velocity （Umf） in the MFBs was 1.67 to 5.25 times higher than that calculated using the Ergun equation when the ratio of solid particle diameter to bed diameter varied from 0.017 to 0.091. The ratio of the Richardson-Zaki （R-Z） exponent obtained by fitting with experimental data to that calculated using the R-Z correlation varied from 0.92 to 0.55. A wider solid particle size distribution resulted in a smaller R-Z exponent. The influence of the solid particle material on Umf and R-Z exponent was negligible.

Gas-liquid mass transfer in the gas-liquid-solid mini fluidized beds

The gas-liquid-solid mini fluidized bed(GLSMFB)combines the advantages of fluidized bed and micro-reactor,and meets the requirements for safety and efficiency of green development of process industry.However,there are few studies on its flow performance and no studies on its mass and heat transfer performance.In this paper,the characteristics of gas-liquid mass transfer in a GLSMFB were studied in order to provide basic guidance for the study of GLSMFB reaction performance and application.Using CO_(2)absorption by NaOH as the model process,the gas-liquid mass transfer performance of GLSMFB was investigated.The results show that the liquid volumetric mass transfer coefficient and the gas-liquid interfacial area both increase with the increase of the superficial gas velocity within the experimental parameter range under the same given superficial liquid velocity.At the same ratio of superficial gas to liquid velocity,the liquid volumetric mass transfer coefficient increases with the increase of the super-ficial liquid velocity.Fluidized solid particles strengthen the liquid mass transfer process,and the liquid volumetric mass transfer coefficient is about 13%higher than that of gas-liquid mini bubble column.

New measurements on hydrodynamics in a gas-liquid-solid expanded bed

The solid holdup,in a 150 mm-ID×2460 mm-height gas-liquid-solid expanded bed with air,water and glass beads(the diameter of particles is 0.6-0.8 mm)was firstly investigated based on the immersion-type online multiphase measuring instrument,and bubble behavior was studied via the BVW-2 double electrical conductivity probe.The effect of the superficial gas velocity and liquid velocity on the expanded ratio,the transition ratio,the bubble rising velocity,the gas holdup and the solid holdup was studied.It is discovered that compared with the gas velocity,the liquid velocity has stronger impact on the expanded ratio,but it is opposite for the transition ratio.The average gas holdup and solid holdup increase linearly as the superficial gas velocity goes up.Among it,the gas holdup increases greater in the center,while the solid holdup increases greater near the wall.Compared with it,when the superficial liquid velocity rises,the average gas holdup hardly changes,but the average solid holdup keeps decreasing,especially the solid holdup distributes flatter with the increase of the superficial liquid velocity.