[Objectives]This study aimed to optimize the siRNA transfection method for ARID4B gene and lay a foundation for further study on the effect of ARID4B low expression on the biological function of liver cancer cells Hep...[Objectives]This study aimed to optimize the siRNA transfection method for ARID4B gene and lay a foundation for further study on the effect of ARID4B low expression on the biological function of liver cancer cells HepG2.[Methods]HepG2 cells were cultured for 0,4 and 24 h,respectively and then transfected with ARID4B targeting siRNA.The mRNA expression of ARID4B gene was detected by RT-qPCR,and the protein expression was detected by Western blot.[Results]When HepG2 cells were transfected at 0 h after seeding,the expression of ARID4B was the lowest in both mRNA and protein levels,that is,the inhibition effect was the best.[Conclusions]Transfection at different time points after seeding of HepG2 cells had different inhibition effect on the expression of ARID4B.Transfection at 0 h after seeding showed the best inhibition effect.展开更多
Successive gliding of twinning disconnections(TDs)creates three-dimensional twins in parent crystal and accommodates shear deformation.It is generally recognized that TD is subject to the same Peierls stress as it gli...Successive gliding of twinning disconnections(TDs)creates three-dimensional twins in parent crystal and accommodates shear deformation.It is generally recognized that TD is subject to the same Peierls stress as it glides forward or backward because of its dislocation character and the twofold rotation symmetry of the twin plane.Based on atomistic simulations,we demonstrate that the glide of TDs may be subject to a symmetric or asymmetric resistance corresponding to step character,symmetric resistance for A/A type steps but asymmetric resistance for A/B type steps,where A and B represent crystallographic planes in twin and matrix.Furthermore,we experimentally demonstrate that the asymmetric resistance results in asymmetric propagation and growth of twins in Mg alloys.展开更多
基金Scientific Research Foundation of Chengde Medical University for High-level Talents(10204030004).
文摘[Objectives]This study aimed to optimize the siRNA transfection method for ARID4B gene and lay a foundation for further study on the effect of ARID4B low expression on the biological function of liver cancer cells HepG2.[Methods]HepG2 cells were cultured for 0,4 and 24 h,respectively and then transfected with ARID4B targeting siRNA.The mRNA expression of ARID4B gene was detected by RT-qPCR,and the protein expression was detected by Western blot.[Results]When HepG2 cells were transfected at 0 h after seeding,the expression of ARID4B was the lowest in both mRNA and protein levels,that is,the inhibition effect was the best.[Conclusions]Transfection at different time points after seeding of HepG2 cells had different inhibition effect on the expression of ARID4B.Transfection at 0 h after seeding showed the best inhibition effect.
文摘Successive gliding of twinning disconnections(TDs)creates three-dimensional twins in parent crystal and accommodates shear deformation.It is generally recognized that TD is subject to the same Peierls stress as it glides forward or backward because of its dislocation character and the twofold rotation symmetry of the twin plane.Based on atomistic simulations,we demonstrate that the glide of TDs may be subject to a symmetric or asymmetric resistance corresponding to step character,symmetric resistance for A/A type steps but asymmetric resistance for A/B type steps,where A and B represent crystallographic planes in twin and matrix.Furthermore,we experimentally demonstrate that the asymmetric resistance results in asymmetric propagation and growth of twins in Mg alloys.
