Many viruses can cause respiratory diseases in humans.Although great advances have been achieved in methods of diagnosis,it remains challenging to identify pathogens in unexplained pneumonia(UP) cases.In this study,we...Many viruses can cause respiratory diseases in humans.Although great advances have been achieved in methods of diagnosis,it remains challenging to identify pathogens in unexplained pneumonia(UP) cases.In this study,we applied next-generation sequencing(NGS) technology and a metagenomic approach to detect and characterize respiratory viruses in UP cases from Guizhou Province,China.A total of 33 oropharyngeal swabs were obtained from hospitalized UP patients and subjected to NGS.An unbiased metagenomic analysis pipeline identified 13 virus species in 16 samples.Human rhinovirus C was the virus most frequently detected and was identified in seven samples.Human measles virus,adenovirus B 55 and coxsackievirus A10 were also identified.Metagenomic sequencing also provided virus genomic sequences,which enabled genotype characterization and phylogenetic analysis.For cases of multiple infection,metagenomic sequencing afforded information regarding the quantity of each virus in the sample,which could be used to evaluate each viruses' role in the disease.Our study highlights the potential of metagenomic sequencing for pathogen identification in UP cases.展开更多
Summary What is already known on this topic?Brucellosis is a zoonotic infectious disease caused by Brucella spp.The main source of infection in human brucellosis is sick animals,mainly including sheep,goat,and cattle,...Summary What is already known on this topic?Brucellosis is a zoonotic infectious disease caused by Brucella spp.The main source of infection in human brucellosis is sick animals,mainly including sheep,goat,and cattle,but sika deer(Cervus nippon)can also cause human brucellosis.The first human brucellosis case in Guizhou Province was reported in 2009,and no brucellosis outbreak was reported caused by sika deer ever before.展开更多
基金supported by the National Mega-projects for Infectious Diseases(2014ZX10004002)Beijing Municipal Science and Technology Commission grant(D151100002115003)
文摘Many viruses can cause respiratory diseases in humans.Although great advances have been achieved in methods of diagnosis,it remains challenging to identify pathogens in unexplained pneumonia(UP) cases.In this study,we applied next-generation sequencing(NGS) technology and a metagenomic approach to detect and characterize respiratory viruses in UP cases from Guizhou Province,China.A total of 33 oropharyngeal swabs were obtained from hospitalized UP patients and subjected to NGS.An unbiased metagenomic analysis pipeline identified 13 virus species in 16 samples.Human rhinovirus C was the virus most frequently detected and was identified in seven samples.Human measles virus,adenovirus B 55 and coxsackievirus A10 were also identified.Metagenomic sequencing also provided virus genomic sequences,which enabled genotype characterization and phylogenetic analysis.For cases of multiple infection,metagenomic sequencing afforded information regarding the quantity of each virus in the sample,which could be used to evaluate each viruses' role in the disease.Our study highlights the potential of metagenomic sequencing for pathogen identification in UP cases.
文摘Summary What is already known on this topic?Brucellosis is a zoonotic infectious disease caused by Brucella spp.The main source of infection in human brucellosis is sick animals,mainly including sheep,goat,and cattle,but sika deer(Cervus nippon)can also cause human brucellosis.The first human brucellosis case in Guizhou Province was reported in 2009,and no brucellosis outbreak was reported caused by sika deer ever before.
