Actinidia eriantha is a characteristic fruit tree featuring with great potential for its abundant vitamin C and strong disease resistance.It has been used in a wide range of breeding programs and functional genomics s...Actinidia eriantha is a characteristic fruit tree featuring with great potential for its abundant vitamin C and strong disease resistance.It has been used in a wide range of breeding programs and functional genomics studies.Previously published genome assemblies of A.eriantha are quite fragmented and not highly contiguous.Using multiple sequencing strategies,we get the haplotype-resolved and gap-free genomes of an elite breeding line“Midao 31”(MD),termed MDHAPA and MDHAPB.The new assemblies anchored to 29 pseudochromosome pairs with a length of 619.3 Mb and 611.7 Mb,as well as resolved 27 and 28 gap-close chromosomes in a telomere-to-telomere(T2T)manner.Based on the haplotype-resolved genome,we found that most alleles experienced purifying selection and coordinately expressed.Owing to the high continuity of assemblies,we defined the centromeric regions of A.eriantha,and identified the major repeating monomer,which is designated as Ae-CEN153.This resource lays a solid foundation for further functional genomics study and horticultural traits improvement in kiwifruit.展开更多
基金Open access funding provided by Shanghai Jiao Tong Universitysupported by funds from the National Natural Science Foundation of China(31972474,31471157).
文摘Actinidia eriantha is a characteristic fruit tree featuring with great potential for its abundant vitamin C and strong disease resistance.It has been used in a wide range of breeding programs and functional genomics studies.Previously published genome assemblies of A.eriantha are quite fragmented and not highly contiguous.Using multiple sequencing strategies,we get the haplotype-resolved and gap-free genomes of an elite breeding line“Midao 31”(MD),termed MDHAPA and MDHAPB.The new assemblies anchored to 29 pseudochromosome pairs with a length of 619.3 Mb and 611.7 Mb,as well as resolved 27 and 28 gap-close chromosomes in a telomere-to-telomere(T2T)manner.Based on the haplotype-resolved genome,we found that most alleles experienced purifying selection and coordinately expressed.Owing to the high continuity of assemblies,we defined the centromeric regions of A.eriantha,and identified the major repeating monomer,which is designated as Ae-CEN153.This resource lays a solid foundation for further functional genomics study and horticultural traits improvement in kiwifruit.
