Fusarium head blight(FHB) is a global wheat disease that devastates wheat production. Resistance to FHB spread within a wheat spike(type Ⅱ resistance) and to mycotoxin accumulation in infected kernel(type Ⅲ resistan...Fusarium head blight(FHB) is a global wheat disease that devastates wheat production. Resistance to FHB spread within a wheat spike(type Ⅱ resistance) and to mycotoxin accumulation in infected kernel(type Ⅲ resistance) are the two main types of resistance. Of hundreds of QTL that have been reported, only a few can be used in wheat breeding because most show minor and/or inconsistent effects in different genetic backgrounds. We describe a new strategy for identifying robust and reliable meta-QTL(mQTL)that can be used for improvement of wheat FHB resistance. It involves integration of mQTL analysis with mQTL physical mapping and identification of single-copy markers and candidate genes. Using metaanalysis, we consolidated 625 original QTL from 113 publications into 118 genetic map-based mQTL(gmQTL). These gmQTL were further located on the Chinese Spring reference sequence map. Finally, 77 high-confidence mQTL(hcmQTL) were selected from the reference sequence-based mQTL(smQTL).Locus-specific single nucleotide polymorphism(SNP) and simple sequence repeat(SSR) markers and17 genes responsive to FHB were then identified in the hcmQTL intervals by combined analysis of transcriptomic and proteomic data. This work may lead to a comprehensive molecular breeding platform for improving wheat resistance to FHB.展开更多
基金supported by the National Key R&D Program,Intergovernmental Key Items for International Scientific and Technological Innovation Cooperation(2018YFE0107700)the National Natural Science Foundation of China(31771772)+2 种基金the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX19_2109)the National Key R&D Program for Breeding of Top-seven Crops(2017YFD0100801)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Fusarium head blight(FHB) is a global wheat disease that devastates wheat production. Resistance to FHB spread within a wheat spike(type Ⅱ resistance) and to mycotoxin accumulation in infected kernel(type Ⅲ resistance) are the two main types of resistance. Of hundreds of QTL that have been reported, only a few can be used in wheat breeding because most show minor and/or inconsistent effects in different genetic backgrounds. We describe a new strategy for identifying robust and reliable meta-QTL(mQTL)that can be used for improvement of wheat FHB resistance. It involves integration of mQTL analysis with mQTL physical mapping and identification of single-copy markers and candidate genes. Using metaanalysis, we consolidated 625 original QTL from 113 publications into 118 genetic map-based mQTL(gmQTL). These gmQTL were further located on the Chinese Spring reference sequence map. Finally, 77 high-confidence mQTL(hcmQTL) were selected from the reference sequence-based mQTL(smQTL).Locus-specific single nucleotide polymorphism(SNP) and simple sequence repeat(SSR) markers and17 genes responsive to FHB were then identified in the hcmQTL intervals by combined analysis of transcriptomic and proteomic data. This work may lead to a comprehensive molecular breeding platform for improving wheat resistance to FHB.
