Integrated biomarker response to assess toxic impacts of iron and manganese on deep-sea mussel Gigantidas platifrons under a deep-sea mining activity scenario

Deep-sea mining activities can potentially release metals,which pose a toxicological threat to deep-sea ecosystems.Nevertheless,due to the remoteness and inaccessibility of the deep-sea biosphere,there is insufficient knowledge about the impact of metal exposure on its inhabitants.In this study,deep-sea mussel Gigantidas platifrons,a commonly used deep-sea toxicology model organism,was exposed to manganese(100,1000μg/L)or iron(500,5000μg/L)for 7 d,respectively.Manganese and iron were chosen for their high levels of occurrence within deep-sea deposits.Metal accumulation and a battery of biochemical biomarkers related to antioxidative stress in superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA);immune function in alkaline phosphatase(AKP),acid phosphatase(ACP);and energy metabolism in pyruvate kinase(PK)and hexokinase(HK)were assessed in mussel gills.Results showed that deep-sea mussel G.platifrons exhibited a high capacity to accumulate Mn/Fe.In addition,most tested biochemical parameters were altered by metal exposure,demonstrating that metals could induce oxidative stress,suppress the immune system,and affect energy metabolism of deep-sea mussels.The integrated biomarker response(IBR)approach indicated that the exposure to Mn/Fe had a negative impact on deep-sea mussels,and Mn demonstrated a more harmful impact on deep-sea mussels than Fe.Additionally,SOD and CAT biomarkers had the greatest impact on IBR values in Mn treatments,while ACP and HK were most influential for the low-and high-dose Fe groups,respectively.This study represents the first application of the IBR approach to evaluate the toxicity of metals on deep-sea fauna and serves as a crucial framework for risk assessment of deep-sea mining-associated metal exposure.

Low-Rate DoS Attack Flows Filtering Based on Frequency Spectral Analysis

In frequency domain,the power spectrum of Low-rate denial of service(LDoS) attacks is totally spread into the spectrum of normal traffic.It is a challenging task to detect and filter LDoS attack flows from the normal traffic.Based on the analysis of LDoS attack flows and legitimate TCP traffic in time and frequency domains,the periodicity of the TCP traffic and LDoS attack flows is explored to facilitate the research of network traffic processing.Hence,an approach of LDoS attack flow filtering based on frequency spectrum analysis is proposed.In this approach,the TCP traffic and LDoS attack flows are transformed from the time domain into the frequency domain.Then the round-trip time(RTT) is estimated by using frequency domain search method.Analysis of amplitude spectrum shows that TCP traffic energy is mainly concentrated on the points of n/RTT.Therefore,a comb filter using infinite impulse response(IIR) filter is designed to filter out the LDoS attack flows in frequency domain,while most legitimate TCP traffic energy at the points of n/RTT are pass through.Experimental results show that the maximum pass rate for legitimate TCP traffic reaches 92.55%,while the maximum filtration rate of LDoS attack flows reaches 81.36%.The proposed approach can effectively filter the LDoS attack flows while less impact on the legitimate TCP traffic.