Tryptophan derivatives have long been used as site-specific biological probes. 4-Cyanotrypto- phan emits in the visible region and is the smallest blue fluorescent amino acid probe for bio- logical applications. Other...Tryptophan derivatives have long been used as site-specific biological probes. 4-Cyanotrypto- phan emits in the visible region and is the smallest blue fluorescent amino acid probe for bio- logical applications. Other indole or tryptophan analogs may emit at even longer wavelengths than 4-cyanotryptophan. We performed FTIR, UV-Vis, and steady-state and time-resolved fluorescence spectroscopy on six ester-derivatized indoles in different solvents. Methyl indole- 4-carboxylate emits at 450 nrn with a long fluorescence lifetirne, and is a promising candidate for a fluorescent probe. The ester-derivatized indoles could be used as spectroscopic probes to study local protein environments. Our measurements provide a guide for choosing esterderivatized indoles to use in practice and data for computational modeling of the effect of substitution on the electronic transitions of indole.展开更多
This paper studies a routing protocol used in the application of collecting real-time traffic information using mobile vehicles to monitor traffic status. The biggest challenge of vehicular ad hoe network routing prot...This paper studies a routing protocol used in the application of collecting real-time traffic information using mobile vehicles to monitor traffic status. The biggest challenge of vehicular ad hoe network routing protocol lies in the mismatch between the direction of moving vehicles and routed data. In order to deal with this mismatch, a mesh based routing protocol with a two-tier network architecture is proposed. By using mesh nodes deployed at intersections, data can be routed through an optimized path which can improve the delivery ratio and reduce the consumption of network resources. The simulation uses the mobility model processed from the GPS data of taxis mnning in Shanghai urban areas. The result shows that the proposed protocol outperforms the existing flooding protocol.展开更多
基金supported by Beijing Natural Science Foundation(L172028)the National Natural Science Foundation of China(No.21773012 and No.91753118)+1 种基金the Recruitment Program of Global Youth Expertsthe Fundamental Research Funds for Central Universities
文摘Tryptophan derivatives have long been used as site-specific biological probes. 4-Cyanotrypto- phan emits in the visible region and is the smallest blue fluorescent amino acid probe for bio- logical applications. Other indole or tryptophan analogs may emit at even longer wavelengths than 4-cyanotryptophan. We performed FTIR, UV-Vis, and steady-state and time-resolved fluorescence spectroscopy on six ester-derivatized indoles in different solvents. Methyl indole- 4-carboxylate emits at 450 nrn with a long fluorescence lifetirne, and is a promising candidate for a fluorescent probe. The ester-derivatized indoles could be used as spectroscopic probes to study local protein environments. Our measurements provide a guide for choosing esterderivatized indoles to use in practice and data for computational modeling of the effect of substitution on the electronic transitions of indole.
基金Supported by the National Basic Research Program of China (No. 2006CB303000)the National Natural Science Foundation of China (No. 60773091, F020303).
文摘This paper studies a routing protocol used in the application of collecting real-time traffic information using mobile vehicles to monitor traffic status. The biggest challenge of vehicular ad hoe network routing protocol lies in the mismatch between the direction of moving vehicles and routed data. In order to deal with this mismatch, a mesh based routing protocol with a two-tier network architecture is proposed. By using mesh nodes deployed at intersections, data can be routed through an optimized path which can improve the delivery ratio and reduce the consumption of network resources. The simulation uses the mobility model processed from the GPS data of taxis mnning in Shanghai urban areas. The result shows that the proposed protocol outperforms the existing flooding protocol.
