A dietary intervention study was assessed to determine if different sources of starch in homemade diets could significantly modify fecal microbiome of dogs.Twenty-seven adult dogs were enrolled and fed a diet based on...A dietary intervention study was assessed to determine if different sources of starch in homemade diets could significantly modify fecal microbiome of dogs.Twenty-seven adult dogs were enrolled and fed a diet based on a mixture of rice and pasta with fresh raw meat(CD).After 90 d,8 dogs continued to receive CD diet,10 dogs received a diet made of a raw meat and a complementary food with rice as the main source of starch(B1),and 9 dogs were fed a diet with the same raw meat and a complementary food with potato as the main source of starch(B2).Samples of feces were collected from each dog in the mornings at the beginning of the study and after 15 d and analyzed for pH,ammonia N(N-NH3)and total N,short chain fatty acids(SCFA)and lactic acid.Relative abundance of fecal microbiota was assessed by sequencing and annotating the V3-V4 regions of the 16S rRNA.Total starch intake was similar between diets but differed in the in vitro rate digestion and in the resistant starch,which was higher in B2 than in B1 and CD diets.Dogs fed B2 diet showed lower(P<0.05)N-NH3 and pH but higher(P<0.05)molar proportion of lactic acid.Linear discriminant analysis of the genera relative abundances indicated a significant(P<0.01)increase of SMB53 genus at the end of the study in B1 diet and of Megamonas genus in B1 and B2 diets in comparison to CD diet.These results suggest that changes of starch source in a raw meat-based diet have limited effects on fecal microbiome in healthy dogs,but underline a high variability of microbiota among dogs.展开更多
基金funded from Department of Agrofood,Environmental and Animal Science of the University of Udine,Grant PRID2017
文摘A dietary intervention study was assessed to determine if different sources of starch in homemade diets could significantly modify fecal microbiome of dogs.Twenty-seven adult dogs were enrolled and fed a diet based on a mixture of rice and pasta with fresh raw meat(CD).After 90 d,8 dogs continued to receive CD diet,10 dogs received a diet made of a raw meat and a complementary food with rice as the main source of starch(B1),and 9 dogs were fed a diet with the same raw meat and a complementary food with potato as the main source of starch(B2).Samples of feces were collected from each dog in the mornings at the beginning of the study and after 15 d and analyzed for pH,ammonia N(N-NH3)and total N,short chain fatty acids(SCFA)and lactic acid.Relative abundance of fecal microbiota was assessed by sequencing and annotating the V3-V4 regions of the 16S rRNA.Total starch intake was similar between diets but differed in the in vitro rate digestion and in the resistant starch,which was higher in B2 than in B1 and CD diets.Dogs fed B2 diet showed lower(P<0.05)N-NH3 and pH but higher(P<0.05)molar proportion of lactic acid.Linear discriminant analysis of the genera relative abundances indicated a significant(P<0.01)increase of SMB53 genus at the end of the study in B1 diet and of Megamonas genus in B1 and B2 diets in comparison to CD diet.These results suggest that changes of starch source in a raw meat-based diet have limited effects on fecal microbiome in healthy dogs,but underline a high variability of microbiota among dogs.
