In this paper we described the effect of administrated CoQ10, and alfa-lipoic acid on the concentration of total CoQ10 inplasma end body tissues of eggs laying hens. Organisms raise a complex network of enzymes, metab...In this paper we described the effect of administrated CoQ10, and alfa-lipoic acid on the concentration of total CoQ10 inplasma end body tissues of eggs laying hens. Organisms raise a complex network of enzymes, metabolites and molecules with antioxidant activities in order to prevent oxidative damage of theirs bodies. Adequate blood concentrations of small weight molecules ingested with food and food additives are important for the proper functioning of the antioxidant defense. To test this hypothesis we prepared following experiment. Forty weeks old hens were selected from two genotypes;Ross 308 broiler mothers and Lohmann breed hens. Animals were fed for a period of 84 days. Concentrations of supplemented CoQ10 and ALAwere calculated from feed instruction tables so each hen received an average of approximately 5 mg of CoQ10 and 50 mg ofALAper kg of animal weight per day. During the experiment blood samples were taken and at the end of the experiment different body tissues (heart, liver, breast, legs) were collected and analyzed with originally developed HPLC-MS/MS method based selective ionization with LiCl on MRM scanning. We found a number of interesting and unexpected results. Supplemented CoQ10 increased concentrations of coenzyme CoQ10 inplasma and different hen’s tissues. Increased concentration of CoQ10 is the result of its transfer with chylomicrons from the digestive tract to various organs of the body and to the liver where exogenous and endogenous CoQ10 has been re-redistributed through lipoproteins. Supplemented ALA caused much greater concentration of CoQ10 indifferent tissues and plasma then CoQ10. Plausible explanation of our results is such that ALA may regenerates the antioxidants and accelerate the formation of endogenous CoQ10 which is distributed with lipoprotein carriers and increases overall concentration of CoQ10. Our experiments definitely show that Lipoic acid beside glutathione promotes also a synthesis of CoQ10 and increases the total concentration especially in liver and heart tissues.展开更多
Antioxidants in the body are in balance, and form a network that protects living organisms against oxidative stress. To prove this hypothesis, we developed suitable methods for assessing the amount of antioxidants and...Antioxidants in the body are in balance, and form a network that protects living organisms against oxidative stress. To prove this hypothesis, we developed suitable methods for assessing the amount of antioxidants and their antioxidant activity in the chickens’ plasma, which enable us to explain the overall anti-oxidant status of the animals during the controlled industrial raise. The effects of exogenous coenzyme Q10, and α-lipoic acid on the reduction of oxidative stress in the animal body were assessed. The physical and the health conditions of chickens during the raising period of 40 days were followed. The benefits of additives were estimated through the measured concentrations of selected low molecular weight antioxi-dants (coenzyme Q10, α-lipoic acid, α-tocopherol, lutein and zeaxathin) and antioxidant enzymes (super-oxide dismutase, glutathione reductase), cholesterol and glucose in plasma and measurements of total antioxidant capacity. Quantitative analyses of selected substances were done with different chromatographic, spectroscopic and electrometric methods. The addition of antioxidants has positive effects on the acts of antioxidant network.展开更多
文摘In this paper we described the effect of administrated CoQ10, and alfa-lipoic acid on the concentration of total CoQ10 inplasma end body tissues of eggs laying hens. Organisms raise a complex network of enzymes, metabolites and molecules with antioxidant activities in order to prevent oxidative damage of theirs bodies. Adequate blood concentrations of small weight molecules ingested with food and food additives are important for the proper functioning of the antioxidant defense. To test this hypothesis we prepared following experiment. Forty weeks old hens were selected from two genotypes;Ross 308 broiler mothers and Lohmann breed hens. Animals were fed for a period of 84 days. Concentrations of supplemented CoQ10 and ALAwere calculated from feed instruction tables so each hen received an average of approximately 5 mg of CoQ10 and 50 mg ofALAper kg of animal weight per day. During the experiment blood samples were taken and at the end of the experiment different body tissues (heart, liver, breast, legs) were collected and analyzed with originally developed HPLC-MS/MS method based selective ionization with LiCl on MRM scanning. We found a number of interesting and unexpected results. Supplemented CoQ10 increased concentrations of coenzyme CoQ10 inplasma and different hen’s tissues. Increased concentration of CoQ10 is the result of its transfer with chylomicrons from the digestive tract to various organs of the body and to the liver where exogenous and endogenous CoQ10 has been re-redistributed through lipoproteins. Supplemented ALA caused much greater concentration of CoQ10 indifferent tissues and plasma then CoQ10. Plausible explanation of our results is such that ALA may regenerates the antioxidants and accelerate the formation of endogenous CoQ10 which is distributed with lipoprotein carriers and increases overall concentration of CoQ10. Our experiments definitely show that Lipoic acid beside glutathione promotes also a synthesis of CoQ10 and increases the total concentration especially in liver and heart tissues.
文摘Antioxidants in the body are in balance, and form a network that protects living organisms against oxidative stress. To prove this hypothesis, we developed suitable methods for assessing the amount of antioxidants and their antioxidant activity in the chickens’ plasma, which enable us to explain the overall anti-oxidant status of the animals during the controlled industrial raise. The effects of exogenous coenzyme Q10, and α-lipoic acid on the reduction of oxidative stress in the animal body were assessed. The physical and the health conditions of chickens during the raising period of 40 days were followed. The benefits of additives were estimated through the measured concentrations of selected low molecular weight antioxi-dants (coenzyme Q10, α-lipoic acid, α-tocopherol, lutein and zeaxathin) and antioxidant enzymes (super-oxide dismutase, glutathione reductase), cholesterol and glucose in plasma and measurements of total antioxidant capacity. Quantitative analyses of selected substances were done with different chromatographic, spectroscopic and electrometric methods. The addition of antioxidants has positive effects on the acts of antioxidant network.
