AIM: To characterize the gene expression profiles in differentstages of carcinogenesis of esophageal epithelium.METHODS: A microarray containing 588 cancer relatedgenes was employed to study the gene expression profil...AIM: To characterize the gene expression profiles in differentstages of carcinogenesis of esophageal epithelium.METHODS: A microarray containing 588 cancer relatedgenes was employed to study the gene expression profileat different stages of esophageal squamous cell carcinomaincluding basal cell hyperplasia, high-grade dysplasia,carcinoma in situ, early and late cancer. Principle componentanalysis was performed to search the genes which wereimportant in carcinogenesis.RESULTS: More than 100 genes were up or down regulatedin esophageal epithelial cells during the stages of basal cellhyperplasia, high-grade dysplasia, carcinoma in situ, earlyand late cancer. Principle component analysis identified aset of genes which may play important roles in the tumordevelopment. Comparison of expression profiles betweenthese stages showed that some genes, such as P160ROCK,JNK2, were activated and may play an important role inearly stages of carcinogenesis. CONCLUSION: These findings provided an esophagealcancer-specific and stage-specific expression profiles,showing that complex alterations of gene expression underliethe development of malignant phenotype of esophagealcancer cells.展开更多
AIM: To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotype...AIM: To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.METHODS: Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Hah ethical origin, to perform this casecontrol study. The haplotypes with significant difference in the gene (s) were further analyzed.RFSULTS: Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP,rs436045 in the protein kinase C/ζgene (PRKCZ) was statistically different between the case and control groups (P<0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.CONCLUSION: PRKCZgene may be associated with type 2 diabetes in Hah population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.展开更多
基金China Key Program on Basic Research,No.G1998051021the Chinese Hi-tech R&D program,No.2001AA231310National Natural Science Foundation of China,No.30170519
文摘AIM: To characterize the gene expression profiles in differentstages of carcinogenesis of esophageal epithelium.METHODS: A microarray containing 588 cancer relatedgenes was employed to study the gene expression profileat different stages of esophageal squamous cell carcinomaincluding basal cell hyperplasia, high-grade dysplasia,carcinoma in situ, early and late cancer. Principle componentanalysis was performed to search the genes which wereimportant in carcinogenesis.RESULTS: More than 100 genes were up or down regulatedin esophageal epithelial cells during the stages of basal cellhyperplasia, high-grade dysplasia, carcinoma in situ, earlyand late cancer. Principle component analysis identified aset of genes which may play important roles in the tumordevelopment. Comparison of expression profiles betweenthese stages showed that some genes, such as P160ROCK,JNK2, were activated and may play an important role inearly stages of carcinogenesis. CONCLUSION: These findings provided an esophagealcancer-specific and stage-specific expression profiles,showing that complex alterations of gene expression underliethe development of malignant phenotype of esophagealcancer cells.
基金the National Natural Science Foundation of China, No.39896200,No30170441the National High Technology Research and Development Program,No.2001AA221161,No.2002BA711A05,No.2002BA71 IA10-02+1 种基金The National Program for Key Basic Research Projects,No.G1998051016the Natural Science Foundation of Beijing,No.7002026
文摘AIM: To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.METHODS: Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Hah ethical origin, to perform this casecontrol study. The haplotypes with significant difference in the gene (s) were further analyzed.RFSULTS: Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP,rs436045 in the protein kinase C/ζgene (PRKCZ) was statistically different between the case and control groups (P<0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.CONCLUSION: PRKCZgene may be associated with type 2 diabetes in Hah population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.