How tumor-infiltrating lymphocytes(TILs)that are tumor-specific but functionally tolerant persist in the antigen-expressing tumor tissue is largely unknown.We have previously developed a modified TRansgenic Adenocarci...How tumor-infiltrating lymphocytes(TILs)that are tumor-specific but functionally tolerant persist in the antigen-expressing tumor tissue is largely unknown.We have previously developed a modified TRansgenic Adenocarcinoma of the Mouse Prostate(TRAMP)model where prostate cancer cells express the T-cell epitope SIYRYYGL(SIY)recognized by CD8 T cells expressing the 2C T-cell receptor(TCR)(referred to as TRP-SIY mice).In TRP-SIY mice,activated 2C T cells rapidly become tolerant following infiltration into the prostate tumor.In this study,we show that tolerant 2C T cells persist in the prostate tumor of TRP-SIY mice by proliferating slowly in a tumor-dependent,but antigen-,interleukin(IL)-7-and IL-15-independent manner.We also show that disappearance of 2C T cells from the lymphoid organs of TRP-SIY mice are due to antigen-induced T-cell contraction rather than altered trafficking or generalized T-cell depletion in the mice.Finally,we show that clonal T cells unreactive to SIY are equally capable of persisting in the prostate tumor.These findings suggest that while functional tolerance of TILs is induced by antigen,persistence of tolerant TILs in the tumor tissue is mediated by a novel mechanism:slow proliferation independent of antigen and homeostatic cytokines.These results also allow CD8 T-cell survival in the tumor environment to be compared with T-cell survival in chronic infection.展开更多
基金supported by grants from the National Institutes of Health(F31-AI080286 to MO and CA100875 to JC)UNCF-Merck Graduate Research and NSF Graduate Research Fellowships(to MO)Singapore-MIT Alliance and Koch Research Fund(to JC).
文摘How tumor-infiltrating lymphocytes(TILs)that are tumor-specific but functionally tolerant persist in the antigen-expressing tumor tissue is largely unknown.We have previously developed a modified TRansgenic Adenocarcinoma of the Mouse Prostate(TRAMP)model where prostate cancer cells express the T-cell epitope SIYRYYGL(SIY)recognized by CD8 T cells expressing the 2C T-cell receptor(TCR)(referred to as TRP-SIY mice).In TRP-SIY mice,activated 2C T cells rapidly become tolerant following infiltration into the prostate tumor.In this study,we show that tolerant 2C T cells persist in the prostate tumor of TRP-SIY mice by proliferating slowly in a tumor-dependent,but antigen-,interleukin(IL)-7-and IL-15-independent manner.We also show that disappearance of 2C T cells from the lymphoid organs of TRP-SIY mice are due to antigen-induced T-cell contraction rather than altered trafficking or generalized T-cell depletion in the mice.Finally,we show that clonal T cells unreactive to SIY are equally capable of persisting in the prostate tumor.These findings suggest that while functional tolerance of TILs is induced by antigen,persistence of tolerant TILs in the tumor tissue is mediated by a novel mechanism:slow proliferation independent of antigen and homeostatic cytokines.These results also allow CD8 T-cell survival in the tumor environment to be compared with T-cell survival in chronic infection.
