Many antioxidant rich phytochemical extracts are attracting interest as stress adaptogens. In this regard, several plants are used traditionally and clinically for the management of neurologic disorders. Here, the ant...Many antioxidant rich phytochemical extracts are attracting interest as stress adaptogens. In this regard, several plants are used traditionally and clinically for the management of neurologic disorders. Here, the anti-stress properties of the ethanol extract of medicinal plant Cymbopogon schoenanthus （CSEE）, which grows wild in Southern Tunisia, were investigated, as well as the effects of some of the extracted active compounds on H202-induced cytotoxicity, overproduction of reactive oxygen species （ROS） and adenosine triphosphate （ATP） depletion in human neuroblastoma SH-SYSY cells. Whereas fluorescence intensity due to DCFH-DA （a marker of ROS production） in H2O2-treated SH-SY5Y cells was greater than that in untreated control cells, pretreatrnent with CSEE and its active compounds ameliorated H2O2-induced ROS production. Moreover, H2O2 at 150 μM significantly decreased intracellular ATP levels in SH-SY5Y cells, and pretreatment with CSEE and its active compounds buffered this effect. These in vitro results demonstrate the potential of CSEE to protect against stress-associated disorders.展开更多
Essential oils (EO) from fresh and dry aerial parts of Pituranthos chloranthus (Benth.) Hook and Pituranthos tortuosus (Coss.) Maire were isolated by hydrodistillation and analyzed by GC/MS. The main constituents of t...Essential oils (EO) from fresh and dry aerial parts of Pituranthos chloranthus (Benth.) Hook and Pituranthos tortuosus (Coss.) Maire were isolated by hydrodistillation and analyzed by GC/MS. The main constituents of the EO obtained from fresh herb of P. chloranthus were found to be α-pinene, sabinene, cis-ocimene and myrcene. In dry biomass, a significant increase of the content of some compounds such as α-phellandrene, △,3-carene and β-phellandrene characterized the oil. Minor changes in the chemical composition of the P. tortuosus EOs obtained from fresh or dry herbs and the major constituents were found to be sabinene and myrcene with equilibrate amounts of α-pinene, p-cymene, cis-ocimene, limonene, trans-β-ocimene, γ-terpinene and cis-verbenol. The paper disc diffusion method was used to evaluate the antibacterial activity and results showed an important inhibitory effect of oils obtained from fresh herb against most tested bacteria.展开更多
Aims The survival and ecological distribution of plants in arid habitats are mainly conditioned by water availability and physiological adaptations to withstand drought.In the present study,we have compared the physio...Aims The survival and ecological distribution of plants in arid habitats are mainly conditioned by water availability and physiological adaptations to withstand drought.In the present study,we have compared the physiological responses to drought of two Retama raetam(retama)subspecies from Tunisia,one of them living under the desert climate(subsp.raetam)and the other one growing on the coast(subsp.bovei).Methods To physiologically characterize the two R.raetam subspecies,and to elucidate their main mechanisms underlying their tolerance to drought stress,parameters related to seed germination,growth,photosynthesis(net photosynthetic rate,intracellular CO_(2) concentration,transpiration rate,stomatal conductance and water-use efficiency)and accumulation of osmolytes(proline,glycine betaine[GB]and soluble sugars)were determined in 4-month-old plants subjected to stress for up to 1 month.Important Findings Drought significantly inhibited germination,growth and all the evaluated photosynthetic parameters.Plants of R.raetam subsp.bovei were severely affected by drought after 3 weeks of treatment when photosynthesis rates were up to 7-fold lower than in the controls.At the same time,proline and GB significantly accumulated compared with the irrigated controls,but much less than in R.raetam subsp.raetam;in the latter subspecies,proline and GB increased to levels 24-and 6-fold higher,respectively,than in the corresponding controls.In summary,the population living in the desert region exhibited stronger tolerance to drought stress than that adapted to the semiarid littoral climate,suggesting that tolerance in R.raetam is dependent on accumulation of osmolytes.展开更多
文摘Many antioxidant rich phytochemical extracts are attracting interest as stress adaptogens. In this regard, several plants are used traditionally and clinically for the management of neurologic disorders. Here, the anti-stress properties of the ethanol extract of medicinal plant Cymbopogon schoenanthus （CSEE）, which grows wild in Southern Tunisia, were investigated, as well as the effects of some of the extracted active compounds on H202-induced cytotoxicity, overproduction of reactive oxygen species （ROS） and adenosine triphosphate （ATP） depletion in human neuroblastoma SH-SYSY cells. Whereas fluorescence intensity due to DCFH-DA （a marker of ROS production） in H2O2-treated SH-SY5Y cells was greater than that in untreated control cells, pretreatrnent with CSEE and its active compounds ameliorated H2O2-induced ROS production. Moreover, H2O2 at 150 μM significantly decreased intracellular ATP levels in SH-SY5Y cells, and pretreatment with CSEE and its active compounds buffered this effect. These in vitro results demonstrate the potential of CSEE to protect against stress-associated disorders.
文摘Essential oils (EO) from fresh and dry aerial parts of Pituranthos chloranthus (Benth.) Hook and Pituranthos tortuosus (Coss.) Maire were isolated by hydrodistillation and analyzed by GC/MS. The main constituents of the EO obtained from fresh herb of P. chloranthus were found to be α-pinene, sabinene, cis-ocimene and myrcene. In dry biomass, a significant increase of the content of some compounds such as α-phellandrene, △,3-carene and β-phellandrene characterized the oil. Minor changes in the chemical composition of the P. tortuosus EOs obtained from fresh or dry herbs and the major constituents were found to be sabinene and myrcene with equilibrate amounts of α-pinene, p-cymene, cis-ocimene, limonene, trans-β-ocimene, γ-terpinene and cis-verbenol. The paper disc diffusion method was used to evaluate the antibacterial activity and results showed an important inhibitory effect of oils obtained from fresh herb against most tested bacteria.
基金Dhikra Zayoud's stay in Valencia was supported by a grant from the Tunisian Ministry of Higher Education and Scientific Research.
文摘Aims The survival and ecological distribution of plants in arid habitats are mainly conditioned by water availability and physiological adaptations to withstand drought.In the present study,we have compared the physiological responses to drought of two Retama raetam(retama)subspecies from Tunisia,one of them living under the desert climate(subsp.raetam)and the other one growing on the coast(subsp.bovei).Methods To physiologically characterize the two R.raetam subspecies,and to elucidate their main mechanisms underlying their tolerance to drought stress,parameters related to seed germination,growth,photosynthesis(net photosynthetic rate,intracellular CO_(2) concentration,transpiration rate,stomatal conductance and water-use efficiency)and accumulation of osmolytes(proline,glycine betaine[GB]and soluble sugars)were determined in 4-month-old plants subjected to stress for up to 1 month.Important Findings Drought significantly inhibited germination,growth and all the evaluated photosynthetic parameters.Plants of R.raetam subsp.bovei were severely affected by drought after 3 weeks of treatment when photosynthesis rates were up to 7-fold lower than in the controls.At the same time,proline and GB significantly accumulated compared with the irrigated controls,but much less than in R.raetam subsp.raetam;in the latter subspecies,proline and GB increased to levels 24-and 6-fold higher,respectively,than in the corresponding controls.In summary,the population living in the desert region exhibited stronger tolerance to drought stress than that adapted to the semiarid littoral climate,suggesting that tolerance in R.raetam is dependent on accumulation of osmolytes.
