Cytotoxic effect of methanolic extracts of Fritillaria imperialis bulbs and Eryngium caucasicum leaves on hepatoma and colon cancer cells

Objective: To evaluate antitumor activities of Fritillaria imperialis and Eryngium caucasicum methanolic extracts on human hepatoma (HepG2) and colon cancer (HCT116) cell lines in comparison to human foreskin fibroblasts as the normal cells. Methods: Methanolic extracts of Fritillaria imperialis and Eryngium caucasicum were prepared by the maceration method. The effect of the extracts at various concentrations (100, 200, 400, 600, and 800 μg/mL) on cell survival was evaluated using the MTT method. Besides, fluorescence staining was used to evaluate death patterns of the cells. Results: MTT assay showed that Fritillaria imperialis significantly decreased the viability of all cell lines after 24 and 48 hours of treatments. However, Eryngium caucasicum extract did not show any significant cytotoxicity effect on the cell lines. Fluorescence staining revealed that Fritillaria imperialis induced apoptosis of HCT116 cells at 550 μg/mL. Conclusions: Fritillaria imperialis extract has antiproliferative and cytotoxic effects on HCT116 and HepG2 cancer cells and therefore, may serve as an anticancer agent.

Eco-friendly green synthesis of novel magnetic Fe3O4/SiO2/ZnO-Pr6O11 nanocomposites for photocatalytic degradation of organic pollutant

This study reports the successful so nochemical synthesis of novel Fe3O4/SiO2/ZnO-Pr6O11(Fe/Si/Zn-Pr6O11) nanocomposites using fructose as a green capping agent.The influence of various parameters containing capping agent,power and time of ultrasound irradiation was investigated to reach optimum morphology and size conditions.The products obtained were characterized by transmission electron microscopy(TEM),UV/Vis diffuse reflectance spectroscopy(DRS),Fourier transform infrared spectra(FT-IR),vibration sample magnetometer(VSM),scanning electron microscopy(SEM),energy dispersive X-ray microanalysis(EDS) and X-ray diffraction(XRD).The Fe3O4/SiO2/ZnO-Pr6O11 nanocomposites display remarkably enhanced photocatalytic activity towards rhodamine b degradation(89.6%)and Congo red(84.7%) under UV irradiation compared with the other products.The results illustrate that the photocatalytic efficiency of magnetic nanocomposites is very much higher than pure Pr6O11nanostructures.Magnetic photocatalyst still has good stability after five successive runs.So,these recyclable nanocomposites can play a role in the treatment of both industrial and domestic contaminated water.