Mortality from cardiovascular abnormalities is almost three times more prevalent in the diabetic population than that in the general population. Psidium guajave has been used traditionally for a long time as a medicin...Mortality from cardiovascular abnormalities is almost three times more prevalent in the diabetic population than that in the general population. Psidium guajave has been used traditionally for a long time as a medicinal herb to cure diabetes. Endothelial dependent vasorelaxation effect of Psidium guajave extract on normal aortic rings was observed. This study was designed to investigate the endothelium and nitric oxide roles inPGE-induced vasorelaxation in diabetic rat vessel. Diabetes was induced by a single intraperitoneal injection of streptozotocin. Eight weeks later, superior mesenteric arteries of non-diabetic and diabetic groups were isolated and perfused according to the McGregor method. Prepared vascular beds were constricted with phenylephrine to induce 70% - 75% of maximal constriction. Psidium guajave extract at concentrations of 0.1 to 4 mg/100ml added to the medium and perfusion pressure was recorded. Baseline perfusion pressure of diabetic group was significantly higher than non-diabetic rats in both intact and denuded endothelium. Psidium guajave extract caused a significantly dose depended decrease in perfusion pressure in non-diabetic and diabetic groups in both intact and denuded endothelium. In the presence of N (ω)-nitro-L-arginine methyl ester, Psidium guajave ex-tractinduced relaxation in intact mesenteric beds of non-diabetic and diabetic animals was not suppressed. From the results of this study, it may be concluded that vasorelaxatory effect of Psidium guajave extract is not mediated by endothelium and nitric oxide in both diabetic and non-diabetic vessel and supports the use of the plant’s leaves as a natural, adjunct phytomedicine in the management of diabetes vessel complications.展开更多
The leaf of Psidium guava is traditionally used in Asia to manage, control and treat diabetes. We designed this study to elucidate the effect of the administration of oral doses of aqueous and ethanol extract from Psi...The leaf of Psidium guava is traditionally used in Asia to manage, control and treat diabetes. We designed this study to elucidate the effect of the administration of oral doses of aqueous and ethanol extract from Psidium guava leaves on plasma glucose, lipid profiles and the sensitivity of the vascular mesenteric bed to Phenylephrine in diabetic and non diabetic rats. Animals were divided into 5 groups (n = 10): two groups served as non-diabetic controls (NDC), while the other groups had diabetes induced with a single injection of streptozotocin (STZ). Psidium guava-treated chronic diabetic (PSG-CD) and Psidium guava-treated controls (PSG-C) received 1g/l of Psidium guavaadded to the drinking water for 8 weeks. The mesenteric vascular beds were prepared using the McGregor method. Administration of Psidium guava caused Ca/Mg ratio, plasma glucose, high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), total cholesterol and triglyceride concentrations to return to normal levels, and was shown to decrease alteration in vascular reactivity to vasoconstrictor agents. Our results support the hypothesis that Psidium guava could play a role in the management of diabetes and the prevention of vascular complications in STZ-induced diabetic rats.展开更多
文摘Mortality from cardiovascular abnormalities is almost three times more prevalent in the diabetic population than that in the general population. Psidium guajave has been used traditionally for a long time as a medicinal herb to cure diabetes. Endothelial dependent vasorelaxation effect of Psidium guajave extract on normal aortic rings was observed. This study was designed to investigate the endothelium and nitric oxide roles inPGE-induced vasorelaxation in diabetic rat vessel. Diabetes was induced by a single intraperitoneal injection of streptozotocin. Eight weeks later, superior mesenteric arteries of non-diabetic and diabetic groups were isolated and perfused according to the McGregor method. Prepared vascular beds were constricted with phenylephrine to induce 70% - 75% of maximal constriction. Psidium guajave extract at concentrations of 0.1 to 4 mg/100ml added to the medium and perfusion pressure was recorded. Baseline perfusion pressure of diabetic group was significantly higher than non-diabetic rats in both intact and denuded endothelium. Psidium guajave extract caused a significantly dose depended decrease in perfusion pressure in non-diabetic and diabetic groups in both intact and denuded endothelium. In the presence of N (ω)-nitro-L-arginine methyl ester, Psidium guajave ex-tractinduced relaxation in intact mesenteric beds of non-diabetic and diabetic animals was not suppressed. From the results of this study, it may be concluded that vasorelaxatory effect of Psidium guajave extract is not mediated by endothelium and nitric oxide in both diabetic and non-diabetic vessel and supports the use of the plant’s leaves as a natural, adjunct phytomedicine in the management of diabetes vessel complications.
文摘The leaf of Psidium guava is traditionally used in Asia to manage, control and treat diabetes. We designed this study to elucidate the effect of the administration of oral doses of aqueous and ethanol extract from Psidium guava leaves on plasma glucose, lipid profiles and the sensitivity of the vascular mesenteric bed to Phenylephrine in diabetic and non diabetic rats. Animals were divided into 5 groups (n = 10): two groups served as non-diabetic controls (NDC), while the other groups had diabetes induced with a single injection of streptozotocin (STZ). Psidium guava-treated chronic diabetic (PSG-CD) and Psidium guava-treated controls (PSG-C) received 1g/l of Psidium guavaadded to the drinking water for 8 weeks. The mesenteric vascular beds were prepared using the McGregor method. Administration of Psidium guava caused Ca/Mg ratio, plasma glucose, high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), total cholesterol and triglyceride concentrations to return to normal levels, and was shown to decrease alteration in vascular reactivity to vasoconstrictor agents. Our results support the hypothesis that Psidium guava could play a role in the management of diabetes and the prevention of vascular complications in STZ-induced diabetic rats.
