In vitro antioxidant,cytotoxic,thrombolytic activities and phytochemical evaluation of methanol extract of the A.philippense L.leaves

Objective:To study the leaves of Adiantum philippense L.for their antioxidant,cytotoxicity and thrombolytic activities and to perform phytochemical evaluation.Methods:In-vitro antioxidant activity of extract was studied using DPPH radical scavenging,reducing power,total phenol and total flavonoid content determination assays.The cytotoxic activity was determined using brine shrimp lethality bioassay,thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis.Results:The antioxidant activity of the extracts was found promising.The reducing power of this crude extract increase with the increase of concentration;IC_(50)values of DPPH scavenging activity was(140.00±0.86)μg/mL as compared to ascorbic acid[IC_(50)(130.00±0.76)μg/mL];Total phenol and total flavonoids content were(148.26±0.24)mg/mL and(163.06±0.56)mg/mL respecnvely.In cytotoxicity assay the LC_(50)values of the sample was(106.41±0.78)μg/mL where as for standard vincristin sulphate was(08.50±0.24)μg/mL as a positive control and the extract shows(12.8611.02)%clot lytic whereas standard streptokinase shows(30.86+0.44%clot lytic activity in thrombolytic assay.The phytochemical evaluation indicates the presence of chemical constituents including carbohydrates,alkaloids,saponins,glycosides,flavonoids.Conclusions:This study shows that the methanol extract of leaves of Adiantum philippense L.has bioactivitv but further compound isolation is necessary to confirm the activities of individual compounds.

Automatic and Robust Segmentation of Multiple Sclerosis Lesions with Convolutional Neural Networks

The diagnosis of multiple sclerosis(MS)is based on accurate detection of lesions on magnetic resonance imaging(MRI)which also provides ongoing essential information about the progression and status of the disease.Manual detection of lesions is very time consuming and lacks accuracy.Most of the lesions are difficult to detect manually,especially within the grey matter.This paper proposes a novel and fully automated convolution neural network(CNN)approach to segment lesions.The proposed system consists of two 2D patchwise CNNs which can segment lesions more accurately and robustly.The first CNN network is implemented to segment lesions accurately,and the second network aims to reduce the false positives to increase efficiency.The system consists of two parallel convolutional pathways,where one pathway is concatenated to the second and at the end,the fully connected layer is replaced with CNN.Three routine MRI sequences T1-w,T2-w and FLAIR are used as input to the CNN,where FLAIR is used for segmentation because most lesions on MRI appear as bright regions and T1-w&T2-w are used to reduce MRI artifacts.We evaluated the proposed system on two challenge datasets that are publicly available from MICCAI and ISBI.Quantitative and qualitative evaluation has been performed with various metrics like false positive rate(FPR),true positive rate(TPR)and dice similarities,and were compared to current state-of-the-art methods.The proposed method shows consistent higher precision and sensitivity than other methods.The proposed method can accurately and robustly segment MS lesions from images produced by different MRI scanners,with a precision up to 90%.