Background:Ocular toxoplasmosis(OT)is the most common etiology of posterior uveitis.The high incidence of macular scarring associated with OT is a leading cause of visual morbidity.Serum biomarkers of the disease woul...Background:Ocular toxoplasmosis(OT)is the most common etiology of posterior uveitis.The high incidence of macular scarring associated with OT is a leading cause of visual morbidity.Serum biomarkers of the disease would aid in its diagnosis.This work was designed as a pilot study to detect OT potential biomarkers.Methods:Blood samples were collected from four groups of nine patients each;toxoplasmosis IgG-with no history of uveitis,non-toxoplasmosis uveitic,first episode OT,and symptomatic recurrent OT.Plasma serum was isolated and subjected to proteomics analysis using 2D gel electrophoresis(GE)and surface-enhanced laser desorption ionization mass spectrometry(SELDI-MS).Selected proteins were separated by GE and sequenced using tandem MS.Results:Fifty markers of OT and 46 markers of recurrent disease were discovered by MS;47%were cross-validated;14 biomarkers were selected for verification by 1D-GE.2D-GE analysis yielded 57 differentially expressed bands,20 of which were excised and identified.One serum protein,peptidyl-prolyl cis-trans isomerase A,was validated to be a biomarker of multi-episodic OT by immunoblotting in patient and control samples.Conclusions:This pilot study sought,for the first time,to elucidate plasma serum biomarkers for OT.This study demonstrates the potential for SELDI-MS and well as other MS technologies to identify novel disease biomarkers.展开更多
文摘Background:Ocular toxoplasmosis(OT)is the most common etiology of posterior uveitis.The high incidence of macular scarring associated with OT is a leading cause of visual morbidity.Serum biomarkers of the disease would aid in its diagnosis.This work was designed as a pilot study to detect OT potential biomarkers.Methods:Blood samples were collected from four groups of nine patients each;toxoplasmosis IgG-with no history of uveitis,non-toxoplasmosis uveitic,first episode OT,and symptomatic recurrent OT.Plasma serum was isolated and subjected to proteomics analysis using 2D gel electrophoresis(GE)and surface-enhanced laser desorption ionization mass spectrometry(SELDI-MS).Selected proteins were separated by GE and sequenced using tandem MS.Results:Fifty markers of OT and 46 markers of recurrent disease were discovered by MS;47%were cross-validated;14 biomarkers were selected for verification by 1D-GE.2D-GE analysis yielded 57 differentially expressed bands,20 of which were excised and identified.One serum protein,peptidyl-prolyl cis-trans isomerase A,was validated to be a biomarker of multi-episodic OT by immunoblotting in patient and control samples.Conclusions:This pilot study sought,for the first time,to elucidate plasma serum biomarkers for OT.This study demonstrates the potential for SELDI-MS and well as other MS technologies to identify novel disease biomarkers.
