Soil oxalate degrading bacteria is an important source for removing of toxic oxalate compounds. Isolation, screening, characterization, identification and fingerprint of the soil oxalate degrading bacteria from Egypti...Soil oxalate degrading bacteria is an important source for removing of toxic oxalate compounds. Isolation, screening, characterization, identification and fingerprint of the soil oxalate degrading bacteria from Egyptian fertile soil was aimed in this work. Isolated bacteria were first characterized using API 20 NE system. Nine RAPD primers were used in genetic fingerprinting of the characterized bacteria using RAPD-PCR. The preliminary characterization and polyphasic taxonomy for mesophilic, aerobic oxalate-degrading bacteria isolated from Egyptian fertile soil including auxanography using API galleries confirmed that the isolate belonged to the genus Burkholderia cepacia, the percentage of identity was 99.8% for 5167777 profiles. Fingerprinting by RAPD-PCR showed a DNA fingerprint ranging from 0 to 9 bands, over a size range of 344 bp to 3,280 bp.展开更多
文摘Soil oxalate degrading bacteria is an important source for removing of toxic oxalate compounds. Isolation, screening, characterization, identification and fingerprint of the soil oxalate degrading bacteria from Egyptian fertile soil was aimed in this work. Isolated bacteria were first characterized using API 20 NE system. Nine RAPD primers were used in genetic fingerprinting of the characterized bacteria using RAPD-PCR. The preliminary characterization and polyphasic taxonomy for mesophilic, aerobic oxalate-degrading bacteria isolated from Egyptian fertile soil including auxanography using API galleries confirmed that the isolate belonged to the genus Burkholderia cepacia, the percentage of identity was 99.8% for 5167777 profiles. Fingerprinting by RAPD-PCR showed a DNA fingerprint ranging from 0 to 9 bands, over a size range of 344 bp to 3,280 bp.
