Objective:To evaluated the histological changes within the tegument of adult Fasciola gigantica(F.gigantica)that led to the gross changes that were visible externally.Methods:The effects of oleoresin extract of myrrh(...Objective:To evaluated the histological changes within the tegument of adult Fasciola gigantica(F.gigantica)that led to the gross changes that were visible externally.Methods:The effects of oleoresin extract of myrrh(Mirazid^(?)),myrrh volatile oil and triclabendazole sulphoxide(reference drug)on the tegumental structure of adult F.gigantiea following treatment in vitro had been determined by light microscopy.Results:The internal changes in the tegument observed in this study were compatible with surface changes seen in the previous scanning electron microscopy study,using the same drugs.The swelling of tegumental syncytium was a particular feature of their action,but its level was much greater with myrrh volatile oil,in which vacuolization of the tegument and loss of spines were observed.Conclusions:The present study demonstrated the fasciocidal properties of Mirazid^(?)oleoresin extract,and it might be possible to reinforce its fasciocidal activity by increasing its content of myrrh volatile oil.展开更多
Objective:To evaluate the molluscicidal activity of chlorophyll extract as a photodynamic substance against the snails Lymnaea stagnalis,Biomphalaria spp.and Physa marmorata.Methods:Chlorophyllin was extracted from de...Objective:To evaluate the molluscicidal activity of chlorophyll extract as a photodynamic substance against the snails Lymnaea stagnalis,Biomphalaria spp.and Physa marmorata.Methods:Chlorophyllin was extracted from deep-frozen spinach.Snails were incubated in chlorophyllin containing water with 2.5,5.0,10.0 and 15.0μg/mL.All samples were kept in darkness overnight for incubation.After incubation,three samples were irradiated with simulated solar radiation for 3 h.After irradiation,the vitality of the organisms was determined.Results:The photodynamically active chlorophyllin,at low concentrations,was able to kill snails within a few hours under exposure of solar radiation.Besides,it had a killing effect by about 70%and 100%on the snails’eggs and the newly hatched snails,respectively,after 3 h exposure to solar radiation.Conclusion:The derivates of chlorophyll was a very interesting substance for photodynamic freshwater snail control.Hence,it might be a promising and cheap new strategy which probably had the potential to replace the synthetic molluscicides for snail control.展开更多
基金Supported by National Research Center,Egypt,through the Parasitology and Animal Diseases Department(Grant No.9040203)
文摘Objective:To evaluated the histological changes within the tegument of adult Fasciola gigantica(F.gigantica)that led to the gross changes that were visible externally.Methods:The effects of oleoresin extract of myrrh(Mirazid^(?)),myrrh volatile oil and triclabendazole sulphoxide(reference drug)on the tegumental structure of adult F.gigantiea following treatment in vitro had been determined by light microscopy.Results:The internal changes in the tegument observed in this study were compatible with surface changes seen in the previous scanning electron microscopy study,using the same drugs.The swelling of tegumental syncytium was a particular feature of their action,but its level was much greater with myrrh volatile oil,in which vacuolization of the tegument and loss of spines were observed.Conclusions:The present study demonstrated the fasciocidal properties of Mirazid^(?)oleoresin extract,and it might be possible to reinforce its fasciocidal activity by increasing its content of myrrh volatile oil.
基金supported by National Research Center,Egypt(Grant No.S-91203).
文摘Objective:To evaluate the molluscicidal activity of chlorophyll extract as a photodynamic substance against the snails Lymnaea stagnalis,Biomphalaria spp.and Physa marmorata.Methods:Chlorophyllin was extracted from deep-frozen spinach.Snails were incubated in chlorophyllin containing water with 2.5,5.0,10.0 and 15.0μg/mL.All samples were kept in darkness overnight for incubation.After incubation,three samples were irradiated with simulated solar radiation for 3 h.After irradiation,the vitality of the organisms was determined.Results:The photodynamically active chlorophyllin,at low concentrations,was able to kill snails within a few hours under exposure of solar radiation.Besides,it had a killing effect by about 70%and 100%on the snails’eggs and the newly hatched snails,respectively,after 3 h exposure to solar radiation.Conclusion:The derivates of chlorophyll was a very interesting substance for photodynamic freshwater snail control.Hence,it might be a promising and cheap new strategy which probably had the potential to replace the synthetic molluscicides for snail control.
