Therapeutic potential of oncolytic viruses in the era of precision oncology

Oncolytic virus(OV)therapy has been shown to be an effective targeted cancer therapy treatment in recent years,providing an avenue of treatment that poses no damage to surrounding healthy tissues.Not only do OVs cause direct oncolysis,but they also amplify both innate and adaptive immune responses generating long-term anti-tumour immunity.Genetically engineered OVs have become the common promising strategy to enhance anti-tumour immunity,safety,and efficacy as well as targeted delivery.The studies of various OVs have been accomplished through phase I-III clinical trial studies.In addition,the uses of carrier platforms of organic materials such as polymer chains,liposomes,hydrogels,and cell carriers have played a vital role in the potentially targeted delivery of OVs.The mechanism,rational design,recent clinical trials,applications,and the development of targeted delivery platforms of OVs will be discussed in this review.

Cellular responses to nanoscale substrate topography of TiO_(2) nanotube arrays: cell morphology and adhesion

Nanotopographical features can be beneficial in augmenting cell functions and increasing osteogenic potential.However,the relationships between surface topographies and biological responses are difficult to establish due to the difficulty in controlling the surface topographical features at a low-nanometre scale.Herein,we report the fabrication of well-defined controllable titanium dioxide(TiO_(2))nanotube arrays with a wide range of pore sizes,30-175 nm in diameter,and use of the electrochemical anodization method to assess the effect of surface nanotopographies on cell morphology and adhesion.The results show that TiO_(2) nanotube arrays with pore sizes of 30 and 80 nm allowed for cell spreading of bone marrow-derived mesenchymal stem cells with increased cell area coverage.Additionally,cell adhesion was significantly enhanced by controlled nanotopographies of TiO_(2) nanotube arrays with 80 nm pore size.Our results demonstrate that surface modification at the nano-scale level with size tunability under controlled chemical/physical properties and culture conditions can greatly impact cell responses.These findings point to a new direction of material design for bone-tissue engineering in orthopaedic applications.

Fate and transport of enveloped viruses in indoor built spaces-through understanding vaccinia virus and surface interactions

The current coronavirus disease 2019(COVID-19)pandemic has reinforced the necessity of understanding and establishing baseline information on the fate and transport mechanisms of viruses under indoor environmental conditions.Mechanisms governing virus interactions in built spaces have thus far been established based on our knowledge on the interaction of inorganic particles in indoor spaces and do not include characteristics specific to viruses.Studies have explored the biological and kinetic processes of microbes’attachments on surfaces in other fields but not in the built environment.There is also extensive literature on the influence of indoor architecture on air flow,temperature profiles,and forces influencing aerosol transport.Bridging the gap between these fields will lead to the generation of novel frameworks,methodologies and know-how that can identify undiscovered pathways taken by viruses and other microbes in the built environment.Our study summarizes the assessment of the influence of surface properties on the adhesion kinetics of vaccinia virus on gold,silica,glass,and stainless-steel surfaces.We found that on gold the virus layer was more viscoelastic compared to stainless-steel.There was negligible removal of the layer from the stainless-steel surface compared to the others.The results further highlight the importance of converging different fields of research to assess the fate and transport of microbes in indoor built spaces.