Molecular Identification of Isolated Bacteria from Soils in Likouala Peat Bog Area, Republic of Congo

This study aimed to the molecular identification of isolated bacteria from the soils of Likouala (Congo-Brazzaville) peat bog area. Counting and isolation were carried out on Mossel and TSB media enriched with petroleum and vegetable oil;the amplification and sequencing of 16S RNA genes by PCR and Artic Oxford Nanopore Technology. The results showed bacterial loads of (5.81 ± 1.08) × 104;(6.64 ± 1.94) × 104 et (8.56 ± 1.19) × 103 CFU/g on Mossel respectively for samples 1, 2 and 3 against (2.12 ± 4.1) × 108 et (8.15 ± 10.1) × 107 CFU/g respectively on TSB enriched with petroleum and vegetable oil exclusively with sample 2. The analysis of the 16S rRNA of the isolates gene made it possible, after PCR, agarose gel electrophoresis, sequencing and bioinformatics analysis, to identify eight (08) strains with similarities of 99 to 100% whose sequences genes placed in GenBank have made it possible to obtain accession numbers corresponding to: Bacillus thuringiensis strain (ON303633);Bacillus cereus strain (ON350770);Bacillus thuringiensis strain (ON350771);Bacillus thuringiensis strain (ON738723);Priestia megaterium strain (ON738719);Bacillus anthracis strain (ON738720);Bacillus subtilis (ON738721);Enterobacter sp (ON738722). The phylogenetic classification of strains was done and revealed two genuses which are Bacillus and Enterobacteriaceae.

Evaluation of Analgesic and Antipyretic Effects of the Aqueous Extract of the Leaves of Chenopodium ambrosioides L. (Amaranthaceae)

C. ambrosioides is a Congolese medicinal plant used in the treatment of several pathologies. This work was initiated to evaluate the analgesic and antipyretic effect of the aqueous extract of leaves of C. ambrosioides. Analgesic effect was evaluated by using the acetic acid-induced writhing, the Tilk Flick test as well as the pain induced by formaldehyde. The results obtained showed that the aqueous extract significantly reduces the number of abdominal writhes (p C. ambrosioides (400 and 600 mg/kg) compared to control group (saline water). This result suggests an antipyretic effect. Chemical screening revealed the presence of alkaloids, flavones and mucilage. The presence of alkaloids and flavones could explain the analgesic and antipyretic effects attributed to this aqueous extract.

Antibiotic Resistance Profile of Pathogenic Bacteria Isolated from “Mabokés” Smothered Fish in Brazzaville, Congo

The smothered fish samples were taken from 3 markets. They were grown on different selective and differentiated culture media to target groups of bacteria associated with food poisoning. Isolates were identified on the basis of cellular and colonial morphologies on selective and differentiated culture media, followed by susceptibility testing to certain families of antibiotics, in particular beta-lactams. This study showed that S. aureus and B. cereus had high levels of beta-lactam resistance. However, these strains were sensitive to kanamycin, tobramycin, ciprofloxacin and norfloxacin. The characteristic penicillinase phenotype was dominant in Gram-positive bacteria. Shigella spp, Salmonella spp and E. coli were resistant to beta-lactam antibiotics. Tobramycin and meropenem retained their activity on all strains. Despite the increased rates of resistance observed, vancomycin, kanamycin, tobramycin, gentamicin, ciprofloxacin, and norfloxacin can be used in the treatment of community-acquired infections caused by Gram-positive bacteria while meropenem and tobramycin for Shigella spp, Salmonella spp and E. coli infections.