Objective:To assess the in vivo antimalarial potential of various solvent extracts and fractions of Trema orientalis.Methods:In this study,the animal model of antimalarial activity was employed using Plasmodium berghe...Objective:To assess the in vivo antimalarial potential of various solvent extracts and fractions of Trema orientalis.Methods:In this study,the animal model of antimalarial activity was employed using Plasmodium berghei-induced mice.The crude methanol extract was fractionated using vacuum liquid chromatography in the order of increasing polarity using dichloromethane,ethylacetate and methanol.Percentages of parasitemia and clearance were used as indices for antiplasmodial activities.The full blood count was also assayed while the gas chromatography-mass spectrometer analysis of the most potent fraction was carried out to detect the active compounds presenting in it.Results:Dichloromethane fraction had the least percentage of parasitemia[(0.19±0.07)%]and the highest percentage of clearance[(91.74±8.38)%]at the highest dose used(200 mg/kg body weight)after day 7 relative to the artemisinin control which cleared the parasite after day 3.The ethylacetate fraction showed the least percentage of clearance[(70.52±5.64)%]at the highest dose used(200 mg/kg body weight)after day 7.Conclusions:The results obtained showed that purification enhanced the antiplasmodial activity of Trema orientalis in Plasmodium berghei-induced malaria in mice.The antiplasmodial activity of the dichloromethane is a strong indication that the fraction,if purified further,may contain drug candidates for the treatment of malaria in the nearest future.展开更多
文摘Objective:To assess the in vivo antimalarial potential of various solvent extracts and fractions of Trema orientalis.Methods:In this study,the animal model of antimalarial activity was employed using Plasmodium berghei-induced mice.The crude methanol extract was fractionated using vacuum liquid chromatography in the order of increasing polarity using dichloromethane,ethylacetate and methanol.Percentages of parasitemia and clearance were used as indices for antiplasmodial activities.The full blood count was also assayed while the gas chromatography-mass spectrometer analysis of the most potent fraction was carried out to detect the active compounds presenting in it.Results:Dichloromethane fraction had the least percentage of parasitemia[(0.19±0.07)%]and the highest percentage of clearance[(91.74±8.38)%]at the highest dose used(200 mg/kg body weight)after day 7 relative to the artemisinin control which cleared the parasite after day 3.The ethylacetate fraction showed the least percentage of clearance[(70.52±5.64)%]at the highest dose used(200 mg/kg body weight)after day 7.Conclusions:The results obtained showed that purification enhanced the antiplasmodial activity of Trema orientalis in Plasmodium berghei-induced malaria in mice.The antiplasmodial activity of the dichloromethane is a strong indication that the fraction,if purified further,may contain drug candidates for the treatment of malaria in the nearest future.
