长波紫外线对人角质形成细胞分泌MMP-1 mRNA MMP-3 mRNA及MMP-9 mRNA的影响

目的研究长波紫外线(Ultraviolet A,UVA)对人角质形成细胞(HaCaT cells)分泌基质金属蛋白酶(Matrix Metalloproteinases,MMPs)MMP-1 mRNA、MMP-3 mRNA及MMP-9 mRNA的影响。方法复苏培养HaCat细胞,绘制细胞生长曲线,确定HaCaT细胞的指数生长期,选用3个剂量(30mJ/cm^2、60 mJ/cm^2、90 mJ/cm^2)进行照射,CCK-8法测定UVA对HaCat细胞增殖活性的影响,qPCR检测HaCat细胞MMP-1 mRNA、MMP-3 mRNA及MMP-9 mRNA的表达水平。采用方差分析,多组均数间两两比较采用q检验,P<0.05为差异有统计学意义。结果经UVA照射后,HaCaT细胞的增殖受到抑制,照光组和未照光组比较差异具有统计学意义(P<0.05)。qPCR扩增后显示MMP-1 mRNA、MMP-3 mRNA及MMP-9 mRNA的表达增加,且以90 mJ/cm^2辐照时表达量最高。结论 HaCaT细胞经UVA照射后,促进MMP-1 mRNA、MMP-3 mRNA及MMP-9 mRNA的分泌,且与辐照剂量呈正相关。

Chinese Experts Consensus on Pre-examination and Triage in Dermatology Clinics during the COVID-19 Outbreak

The SARS-CoV-2 infection has brought a great challenge in prevention and control of the national epidemic of coronavirus disease 2019(COVID-19)in China.During the COVID-19 epidemic,properly carrying out pre-examination and triage for patients with skin lesions and fever has become a practical problem encountered in hospitals for skin diseases and dermatology clinics in general hospitals.Some of the carriers of the SARS-CoV-2 and patients with COVID-19 in the early stage may not present with any symptoms of COVID-19,while certain other skin diseases can also cause fever.Therefore,to properly deal with the patients presenting at dermatology clinics,the Chinese Society of Dermatology organized experts to formulate principles and procedures for the pre-examination and triage of patients at dermatology clinics during the COVID-19 epidemic.