Antioxidant and cytotoxic activity of Acanthus ilicifolius flower

Objective:To investigate the antioxidant and cytotoxic activity of the flower of Acanthus ilicifolius(A.ilicifolius).Methods:Antioxidant activity was determined as antiradical efficiency with diphenyl picrylhydrazil(DPPH)method and cytotoxic assay was undertaken using brine shrimp lethal toxicity test.Results:A.ilicifolius flower contained terpenoid,phenolic compounds,and alkaloid.The methanol extract of A.ilicifolius flower showed the highest antiradical efficiency(AE=1.41×10^(-3))against DPPH radicals and the highest cytotoxicity(LC_(50)=22μg/mL)against brine shrimp nauplii.Conclusions:It is suggested that active compounds of A.ilicifolius flower solved in methanol play a role to inhibit free radical activity and kill Artemia salina nauplii.The substances can be considered as potential antioxidant and cytotoxic agents as well as imminent candidate for cancer therapy.

Sargassum polycystum extract attenuates oxidative stress on diabetic rats

Objective:To evaluate hypoglycemic,free radical scavenging and improving antioxidant enzymes activities on diabetic-induced streptozotocin rats by Sargassum polycystum(S.polycystum)extract.Methods:The seaweed extract was obtained by maceration,concentration and freeze drying,respectively.Acute toxicity was investigated on 25 rats.Forty eight of rats were used to study anti-stress oxidative of extract and divided into eight groups,where the first and fifth group were normal and diabetic control.The normal and diabetic treated groups were administered orally with extracts of S.polycystum for 28 days.The blood glucose and body weight of rats were observed each week.The blood was obtained for determination of malondialdehide,superoxide dismutase,catalase and glutathione peroxidase,respectively.Results:Extract of S.polycystum revealed no mortality and it was grouped as relatively nontoxic substance.The normal rats revealed difference statistically to the diabetic rats.The diabetic rats treated extract showed decreasing of blood glucose level and increasing of body weight.The diabetic rats were treated with 450 mg/kg of extract showed the higher oxidative stress augmentation than other diabetic treatments.It was caused free radical scavenging and induction of antioxidant enzymes activity by brown seaweed extract.Conclusions:The extract of S.echinocarpum reduce oxidative stress on diabetic-induced streptozotocin rats and demonstrate as candidate of antioxidant diabetic substances.