Antioxidant potential of phenolic extracts of Mimusops elengi

Objective:To evaluate the antioxidant potential of the phenolic extracts of Mimusops elengi(M.elengi) L.(Sapotaceae).Methods:The extract of stem bark and seeds of M.elengi were prepared in methanol and acefone:water(7:3).The acetone:water was further partitioned with ethyl acetate and n-butanol.Antioxidant activity of the extracts and partitioned fractions of M.elengi was evaluated in terms of radical scavenging potential(DPPH),inhibition of lipid peroxidation[ferric thiocyanate(FTC)],and total antioxidant activity(phosphomolybdate method).Total phenolics content were calculated using Folin-Ciocalteu reagent.Results:The stem bark extract partitioned with ethyl acetate exhibited highest amount ol total phenols(98.0 mg GAE/g dry weight),among all other extracts,with 92.0%DPPH radical scavenging activity al concentration of0.5 mg/mL.while methanol extract(stem bark) had maximum inhibition ol lipid peroxidation(62.0%)and total antioxidant activity(771.0 mg/g GAE/g).A positive correlation occurred between tolal phenols and radical scavenging activity(R^2 = 0.9229) and total antioxidant activity(R^2= 0.9451).Conclusions:Our study suggested that antioxidant activity of stembark extract of M.elengi is due the presence of phenolic compounds.Furthermore,the hark extract is a valuable source of natural antioxidants.

Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract

Objective:To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum nigrum.Methods:Optimized extraction for maximum recovery of metabolites was performed using a combination of freeze-drying and ultrasonication followed by determination of antioxidant and antiobesity properties.The ultra-high performance liquid chromatography equipped with mass spectrometry was used to analyze metabolite profiling of Solanum nigrum.Computational studies were performed using molecular docking and electrostatic potential analysis for individual compounds.The hypolipidemic potential of the most potent extract was assessed in the obese mice fed on fat rich diet.Results:The 80%hydroethanolic extract exhibited the highest extract yield,total phenolic contents,total flavonoid contents along with the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity,total antioxidant power,and pancreatic lipase inhibitory properties.The 80%hydroethanolic extract not only regulated the lipid profile of obese mice but also restricted the weight gain in the liver,kidney,and heart.The 80%hydroethanolic extract also reduced alanine transaminase and aspartate transaminase concentrations in serum.The effects of plant extract at 300 mg/kg body weight were quite comparable with the standard drug orlistat.Conclusions:Solanum nigrum is proved as an excellent and potent source of secondary metabolites that might be responsible for obesity mitigation.

Structure Elucidation and Biological Screening of Bis(4-{[(E)-(2-Hydroxybenzylidene)amino]methyl}-cyclohexane-carboxylato-O)-triaqua-cadmium

Using a bidentate ligand, a Schiff base 4-（{[（Z）-（2-hydroxyphenyl）methyldene]amino}methyl）cyclohexanecarboxylic acid, complex I（ML2, where M = Cd（Ⅱ）） was synthesized and characterized by IR spectroscopy and X-ray crystallography. It crystallizes in monoclinic system, space group C2/c, M_r = 685.04, 0.34 mm × 0.26 mm × 0.14 mm, a = 54.050（3）, b = 6.1220（3）, c = 9.5081（5） A, β = 90.229（2）°, Z = 4, C_（30） H_（40） CdN_2 O_9 and V = 3146.2（3） A^3. Complex I was screened for its bactericidal activity against four bacterial strains Chromohalobactersalexigens, Chromohalobacterisraelensis, Halomonashalofila and Halomonassalina and fungicidal activity against Aspergillusnigerand Aspergillusflavus. Antimicrobial activity results showed that the synthesized complex displayed good anti-fungal activity against two fungal strains. It was found to be inactive against four different bacterial strains analyzed and was negative for bactericidal effect.