First molecular identification of Cryptosporidium by 18S rRNA in goats and association with farm management in Terengganu

Objective:To identify the prevalence of Cryptosporidium from goats in three types of farm management systems in Terengganu,Malaysia and to determine the Cryptosporidium species infecting goats by using 18 S r RNA.Methods:A total of 478 fecal samples were randomly collected from goats in three farms;199 samples were collected from intensive farm,179 samples from semi-intensive farm and 100 samples from extensive farm.The samples were processed by using formolether concentration technique and stained by using modified Ziehl–Neelsen.Positive samples were performed by using nested PCR analysis by using 18 S r RNA.Results:Out of 478 goats,207(43.3%) were found to be infected with Cryptosporidium.Goats reared under the intensive farm management system reported the highest prevalence of infection(49.7%),followed by intensive farm management system(41%) and the lowest prevalence was reported in the goats reared under semi-intensive management system(37.4%).Conclusions:The identified species found in goat was Cryptosporidium parvum.Future study on the zoonotic transmission of Cryptosporidium parvum in goats needs to be done in order to find the source of transmission of this parasite.

Prevalence of gastrointestinal nematodiasis and coccidiosis in goats from three selected farms in Terengganu,Malaysia

Objective:To reveal the prevalence of gastrointestinal nematodiasis,Trichostrogylus,Haemonchus,Strongyloides and Trichuris and coccidiosis,mainly Eimeria in kids,yearlings and adult goats.Methods:A total of 287 goat faecal specimens were randomly collected from three farms in Kuala Terengganu,Setiu and Besut.Results:Prevalence of coccidiosis(89.2%) was significantly higher(P<0.05) than gastrointestinal nematodiasis(52.3%).Gastrointestinal nematodes(GINs) were found in37.0% kids,63.5% yearlings and 53.3% adults.Identification of GINs revealed the predominance of Strongyloides(45.6%) followed by Trichostrogylus(12.9%),Haemonchus(11.8%) and Trichuris(8.7%) in all groups whereby the adult goats recorded the highest infections of Trichostrogylus(20.5%),Haemonchus(10.6%),Strongyloides(51.5%) in all groups.The age-specific intensity for Eimeria was in following order:kids(92.6%),adults(86.6%) and yearlings(51.5%) and the difference was not significant(P > 0.05).The concurrent infections between GINs and Eimeria were 67.94% in all groups.Polyparasitisms of Trichostrongylus(17.4%),Haemonchus(15.15%) and Strongyloides(40.4%) with Eimeria had infected most adult goats.Dual infections of Trichostrongylus with Eimeria and Trichuris with Eimeria were significantly higher in adults than yearlings and kids(P < 0.05).Conclusions:GINs and Eimeria were widely distributed in the goats leading to higher risk of morbidity and mortality.