Photonic crystal(PC)barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays,such as broad analysis of biomarkers for clinical diagnosis,gene expression,and cell culture.U...Photonic crystal(PC)barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays,such as broad analysis of biomarkers for clinical diagnosis,gene expression,and cell culture.Unfortunately,most of these existing PC barcodes suffered from undesired features,including difficult spectrum-signal acquisition,weak mechanical strength,and high ontology fluorescence,which limited their development to real applications.To address these limitations,we report a new type of structural color-encoded PC barcodes.The barcodes are fabricated by the assembly of monodisperse polydopamine-(PDA-)coated silica(PDA@SiO_(2))nanoparticles using a droplet-based microfluidic technique and followed by pyrolysis of PDA@SiO_(2)(C@SiO_(2))barcodes.Because of the templated carbonization of adhesive PDA,the prepared C@SiO_(2)PC beads were endowed with simultaneous easy-to-identify structural color,high mechanical strength,and ultralow ontology fluorescence.We demonstrated that the structural colored C@SiO_(2)barcodes not only maintained a high structural stability and good biocompatibility during the coculturing with fibroblasts and tumor cells capture but also achieved an enhanced fluorescentreading signal-to-noise ratio in the fluorescence-reading detection.These features make the C@SiO_(2)PC barcodes versatile for expansive application in fluorescence-reading-based multibioassays.展开更多
基金supported by the project funded by the China Postdoctoral Science Foundation(No.2019M652588)National Natural Science Foundation of China(NSFC)Grants(No.32001015,No.21635001)+1 种基金Beijing Science and Technology New Star Project(Z201100006820038)Beihang Youth Top-Notch Talent Support Program(YWF-20-BJ-J-1035)。
文摘Photonic crystal(PC)barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays,such as broad analysis of biomarkers for clinical diagnosis,gene expression,and cell culture.Unfortunately,most of these existing PC barcodes suffered from undesired features,including difficult spectrum-signal acquisition,weak mechanical strength,and high ontology fluorescence,which limited their development to real applications.To address these limitations,we report a new type of structural color-encoded PC barcodes.The barcodes are fabricated by the assembly of monodisperse polydopamine-(PDA-)coated silica(PDA@SiO_(2))nanoparticles using a droplet-based microfluidic technique and followed by pyrolysis of PDA@SiO_(2)(C@SiO_(2))barcodes.Because of the templated carbonization of adhesive PDA,the prepared C@SiO_(2)PC beads were endowed with simultaneous easy-to-identify structural color,high mechanical strength,and ultralow ontology fluorescence.We demonstrated that the structural colored C@SiO_(2)barcodes not only maintained a high structural stability and good biocompatibility during the coculturing with fibroblasts and tumor cells capture but also achieved an enhanced fluorescentreading signal-to-noise ratio in the fluorescence-reading detection.These features make the C@SiO_(2)PC barcodes versatile for expansive application in fluorescence-reading-based multibioassays.
